| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,931,976 | (G)5→6 | coding (1096/1179 nt) | purT → | phosphoribosylglycinamide formyltransferase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,931,971 | 1 | . | G | 94.4% | 118.3 / 0.3 | 36 | coding (1091/1179 nt) | purT | phosphoribosylglycinamide formyltransferase 2 |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (17/17); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01 | |||||||||||
GTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑T‑GGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCC > NC_000913/1931836‑1932088 | gTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑T‑ggg > 1:1139466/1‑139 (MQ=255)gTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑T‑ggg < 1:1307022/139‑1 (MQ=255) tCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGAGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTg > 1:1179690/1‑139 (MQ=255) cTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTgg < 2:120979/139‑1 (MQ=255) aTTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCa < 2:88747/139‑1 (MQ=255) aCTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCaga > 1:360487/1‑130 (MQ=255) aCTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCaga < 2:360487/130‑1 (MQ=255) aCTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGCCGTC‑TGGGGGGTGGCATTGGCTACTGCAGAGGGtgttgt > 2:355969/1‑139 (MQ=255) tCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTgg > 2:42038/1‑111 (MQ=255) tCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTgg < 1:42038/111‑1 (MQ=255) aCGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGaa > 2:725712/1‑133 (MQ=255) aCGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGaa < 1:725712/133‑1 (MQ=255) aCGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAAcgcgcg < 2:1139466/139‑1 (MQ=255) cAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCa > 2:801167/1‑94 (MQ=255) cAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCa > 2:117149/1‑129 (MQ=255) cAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCa < 1:117149/129‑1 (MQ=255) cAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATAGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCa < 1:801167/94‑1 (MQ=255) gAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCGGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACa < 2:1179690/139‑1 (MQ=255) tGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACt < 1:352025/79‑1 (MQ=255) tGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACt > 2:352025/1‑79 (MQ=255) cGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTaaa > 2:1109001/1‑139 (MQ=255) gCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAAc > 1:17758/1‑139 (MQ=255) gCAGATTCGTGTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAAc > 2:946647/1‑139 (MQ=255) cAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCgg < 2:1103763/114‑1 (MQ=255) cAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCgg > 1:1103763/1‑114 (MQ=255) aTTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTa > 2:1313347/1‑124 (MQ=255) aTTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTa < 1:1313347/124‑1 (MQ=255) tCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTT‑GGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACa < 2:397004/112‑1 (MQ=255) tCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTT‑GGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACa > 1:397004/1‑112 (MQ=255) tAAGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTAc < 1:1109001/139‑1 (MQ=255) aGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAAccc > 1:698075/1‑121 (MQ=255) aGCCGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAAccc < 2:698075/121‑1 (MQ=255) cGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACaaaaa > 2:1146680/1‑139 (MQ=255) cGGAAATTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACaaaaa > 1:100208/1‑139 (MQ=255) aaTTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGcc < 1:915468/139‑1 (MQ=255) aaTTGATGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGcc < 1:1146680/139‑1 (MQ=255) aTGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGc < 1:427078/38‑1 (MQ=255) aTGGCAGCCGTCGTC‑TGGGGGGTGGCACTGGCTACTGc > 2:427078/1‑38 (MQ=255) | GTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTC‑T‑GGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCC > NC_000913/1931836‑1932088 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |