| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,950,559 | (C)5→6 | intergenic (‑37/‑273) | aspS ← / → yecD | aspartyl‑tRNA synthetase/isochorismatase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,950,554 | 1 | . | C | 97.7% | 155.2 / ‑3.5 | 44 | intergenic (‑32/‑278) | aspS/yecD | aspartyl‑tRNA synthetase/isochorismatase family protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (22/21); total (22/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
GAAGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTACC > NC_000913/1950418‑1950684 | gAAGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑‑cc > 1:511069/1‑139 (MQ=255) aaGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑‑cc‑c < 2:1164255/139‑1 (MQ=255) ggCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑‑cc‑cccctgct < 2:1176404/139‑6 (MQ=255) tCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGAc > 2:787413/1‑139 (MQ=255) cACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCAGCGGATG‑‑CCGCCCTGCTGCGCAGGTGAcc < 1:877103/139‑1 (MQ=255) acgacgacgGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCa > 1:729704/1‑139 (MQ=255) gacgacgGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAg > 2:325578/1‑139 (MQ=255) gacgacgGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCC‑‑‑‑CTTAGCTGCCGGCGGATG‑‑cc‑cccc < 1:245784/116‑2 (MQ=255) gacgacgGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCC‑‑‑‑CTTAGCTGCCGGCGGATG‑‑cc‑cccc > 2:245784/1‑115 (MQ=255) aCCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑‑cc‑cccctg < 2:48347/106‑4 (MQ=255) aCCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑‑cc‑cccctg > 1:48347/1‑103 (MQ=255) tCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACt > 1:1065170/1‑139 (MQ=255) tCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACt > 2:922353/1‑139 (MQ=255) tCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACt > 1:581325/1‑139 (MQ=255) cACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACtg > 1:634229/1‑139 (MQ=255) gACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCGTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc > 2:927796/1‑133 (MQ=255) gACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc < 1:927796/133‑1 (MQ=255) gACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCtat > 2:1013251/1‑139 (MQ=255) cTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGc > 2:858346/1‑88 (MQ=255) cTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTACCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGc < 1:858346/88‑1 (MQ=255) acaATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGc < 1:188157/139‑1 (MQ=255) aaTATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCg > 2:1074667/1‑139 (MQ=255) tCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc > 1:836205/1‑109 (MQ=255) tCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc < 2:836205/109‑1 (MQ=255) tGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTa < 1:1013251/139‑1 (MQ=255) aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTa < 1:406484/132‑1 (MQ=255) aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTa > 2:406484/1‑132 (MQ=255) aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCt > 2:207901/1‑139 (MQ=255) agaTATCCCTTAACTTAGCTGCCGGTGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGc > 1:3583/1‑139 (MQ=255) gaTATCCCTTAACTTAGCTGCCGGTGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGcc < 2:3583/139‑1 (MQ=255) gaTATCCCTTAACTTAGCTGCCGGCGGATGCCCC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGc < 2:267629/139‑1 (MQ=255) gaTATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGcc < 2:88608/139‑1 (MQ=255) gaTATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGcc < 2:511069/139‑1 (MQ=255) gaTATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGcc < 1:1074667/139‑1 (MQ=255) atatCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGccc > 2:467612/1‑139 (MQ=255) cccTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCg > 2:247937/1‑116 (MQ=255) cccTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCg < 1:247937/116‑1 (MQ=255) ccTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAg < 1:803809/61‑1 (MQ=255) ccTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAg > 2:803809/1‑61 (MQ=255) tAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGa < 1:325578/139‑1 (MQ=255) aaCTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTg > 1:924112/1‑60 (MQ=39) aaCTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTg < 2:924112/60‑1 (MQ=39) tAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGcc > 2:124612/1‑139 (MQ=255) aGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCt > 2:793808/1‑139 (MQ=255) gCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTc < 2:581325/139‑1 (MQ=255) tGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGt < 1:467612/139‑1 (MQ=255) ccGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTa < 2:729704/139‑1 (MQ=255) ccGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTa < 2:94832/139‑1 (MQ=255) cggcggATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAc < 1:787413/139‑1 (MQ=255) ggcggATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAcc < 1:922353/139‑1 (MQ=255) ggcggATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAcc > 2:1180133/1‑139 (MQ=255) | GAAGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTACC > NC_000913/1950418‑1950684 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |