| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,767,507 | (C)7→8 | intergenic (+152/‑203) | rnlB → / → yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,767,500 | 1 | . | C | 85.7% | 143.5 / 15.3 | 49 | intergenic (+145/‑210) | rnlB/yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
| Reads supporting (aligned to +/- strand): ref base . (4/3); new base C (22/20); total (26/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑CCC‑CCC‑CT‑CACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑T‑‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGA > NC_000913/2767367‑2767629 | ctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACCCC‑CCC‑CT‑CCCAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑T‑‑c‑c‑cc < 2:1158622/139‑1 (MQ=255) cAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACCCC‑CCC‑CT‑CACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTaaa < 1:821573/139‑1 (MQ=255) tCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑AAC‑CCC‑CTATAAAACCTCCTTCATAGGGTAAACTTTTTTACATTTGCACCCAACTCCAAAT‑T‑T‑‑CTC‑ATCCCTGTAAACCTCTCcataa > 2:4820/1‑139 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAA‑ACC‑CCC‑CT‑AACAACGTCCTGCATAGAGAACACGTTTGCCACTGTGCAACATAGACTAAAT‑T‑T‑‑C‑CTCCCCCTTTAAAATTCTCAATAATg > 2:814009/1‑139 (MQ=255) aCTTGTCCAATATGATCAAATAGCATT‑AACCCC‑CCC‑CT‑CACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCCCAATAATGTCACCAATTTTCTGGAAATc < 1:814009/139‑1 (MQ=255) tCCAATATGATCAAATAGCATT‑AACCCC‑CCC‑CT‑CACAACTTCCTGCATAGGGTACACGTTTTCACCTGTGCACCCACGACTAAATAT‑C‑‑C‑C‑CCCCCTTTAAACTTCTCATTATTTTCACCAATTTTTTGGACATCTTatg > 2:799778/1‑139 (MQ=255) tCCAATATGATCAAATAGCATT‑AA‑CCCACGC‑CT‑CACAACGCCCTGCCTAGAGAACACGTGTTCCCCTGCGGACGCACGTCTAAAT‑T‑TC‑C‑C‑CCCCCTTTCAGCTTCTCAATAATGTCACCAATGTGCTGGAAATCCTatg > 1:68180/1‑139 (MQ=255) tCCAATATGATCAAATAGCATT‑AA‑CCC‑CCCGCT‑CACAACATCCTGCAAAGGGTACACGATCTCCCCTGTGCACCCACGACTAAAT‑TAA‑‑A‑C‑ACTCCGTTAAACATCTCAATAATGTCACCAAACTTCTggccagcccatg > 2:1094166/1‑129 (MQ=255) aTCAAATAGCATT‑AACCCC‑CCC‑CT‑CACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTc < 1:100072/139‑1 (MQ=255) tCAAATAGCATT‑AACCCC‑CCC‑CT‑CACAACGTCCTTCATAGGGAACACGTTTTCCACTCTGCACCCACGACTCAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGACAACCTATGACGCCttttc > 2:781852/1‑137 (MQ=255) tCAAATAGCATT‑AACCCC‑CCC‑CT‑CACAACGTCCTGCATATGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCCATTTTCTGGAAATCCTATGATGCCTTTcc > 2:116170/1‑139 (MQ=255) aaaTAGCATT‑AACCCC‑CCC‑CT‑CACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCg < 1:4820/139‑1 (MQ=255) aGCATT‑AACCCC‑CCC‑CT‑CACGTCGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCTAAGTCTACAT‑T‑T‑‑C‑CGCCCCCTTTAATCTTCCTAATAAGGTCAGCAATTTTCTGGAAATCCTCTGATGACTTTCCCGGTaa < 2:20993/139‑1 (MQ=255) gCATT‑AACCCC‑CCC‑CT‑CACAACGTCCTGCATAGGGAACACGGTTTCCCCTGTGCACCCACGACTAAATGT‑C‑‑C‑C‑CCCCCTTTAAACCTCTCAATGATGGCCCCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTaaa > 2:902331/1‑139 (MQ=255) aTT‑AACCCC‑CCC‑CT‑CACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCAcc > 2:1276239/1‑100 (MQ=255) aTT‑AACCCC‑CCC‑CT‑CACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCAcc < 1:1276239/100‑1 (MQ=255) aTT‑AACCCC‑CCC‑CT‑CACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATg < 1:1067383/139‑1 (MQ=255) ttaacccc‑ccc‑cT‑CACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TCCC‑C‑CCCCCTTTAAACTTCTCTATAATGTTACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTaaagg > 1:1161682/6‑137 (MQ=255) aaCGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGaa < 1:1073953/139‑1 (MQ=255) tCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGAt > 1:222/1‑139 (MQ=255) ccTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGAtt > 2:898013/1‑139 (MQ=255) cATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGc < 2:222/139‑1 (MQ=255) ggAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTaaa > 1:866352/1‑51 (MQ=255) ggAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTaaa > 1:914678/1‑51 (MQ=255) ggAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTaaa < 2:914678/51‑1 (MQ=255) ggAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTaaa < 2:866352/51‑1 (MQ=255) ggAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCt > 1:1029562/1‑139 (MQ=255) ggAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑T‑‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTa > 2:1010001/1‑139 (MQ=255) acGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAAt < 1:226790/78‑1 (MQ=255) acGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAAt > 2:226790/1‑78 (MQ=255) acGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATg > 1:1277234/1‑103 (MQ=255) acGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATg < 2:1277234/103‑1 (MQ=255) tttCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCAtt > 1:488713/1‑139 (MQ=255) ttCCCCTGTGCCCCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTc < 1:667515/121‑1 (MQ=255) ttCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCt > 1:979533/1‑85 (MQ=255) ttCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTc > 2:667515/1‑121 (MQ=255) ttCCCCTGTGCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCCTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCt < 2:979533/85‑1 (MQ=255) ttCCCCTGTGCACCCACGACTAAAT‑T‑T‑‑C‑C‑CCCCCcttta > 1:811782/1‑37 (MQ=255) ttCCCCTGTGCACCCACGACTAAAT‑T‑T‑‑C‑C‑CCCCCcttta < 2:811782/39‑3 (MQ=255) gtgCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgatg > 1:1021607/1‑75 (MQ=255) gtgCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgatg < 2:1021607/75‑1 (MQ=255) gtgCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAAt > 1:881478/1‑139 (MQ=255) tgCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAg > 2:543771/1‑105 (MQ=255) tgCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAg < 1:543771/105‑1 (MQ=255) tgCACCCACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATc < 2:881478/139‑1 (MQ=255) tgcgttgtttaagaaT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCt > 1:752447/14‑139 (MQ=255) tgcgttgtttaagaaT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCt > 1:357325/14‑139 (MQ=255) ccACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTg < 1:876362/110‑1 (MQ=255) ccACGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTg > 2:876362/1‑110 (MQ=255) aCGACTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCagat < 1:799778/139‑1 (MQ=255) aCTAAATGT‑C‑‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCAGTGAAAAAATCTTCagataga < 2:68180/139‑1 (MQ=255) aCTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCagataga < 1:902331/139‑1 (MQ=255) aCTAAAT‑T‑TC‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCagataga > 2:117172/1‑139 (MQ=255) cTAAAT‑T‑TCCC‑C‑CCCCCTTTAAACTTCGCAATAATGTCACCTATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCagataga < 2:1161682/139‑1 (MQ=255) | CTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑CCC‑CCC‑CT‑CACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑T‑‑C‑C‑CCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGA > NC_000913/2767367‑2767629 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |