| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,970,032 | (G)6→7 | coding (1002/1047 nt) | wzzE → | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,970,026 | 1 | . | G | 93.8% | 220.7 / 5.6 | 64 | coding (996/1047 nt) | wzzE | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Reads supporting (aligned to +/- strand): ref base . (3/1); new base G (27/33); total (30/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑TC‑GGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACG > NC_000913/3969888‑3970159 | ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑Tc > 1:643918/1‑139 (MQ=255)ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑Tc > 1:1234076/1‑139 (MQ=255)ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑Tc > 2:253304/1‑139 (MQ=255) cTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCggg > 1:187832/1‑139 (MQ=255) cTGAATGTTGGTCAAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCGTTG‑TCGGGGGGG‑CTGATCggg > 1:771448/1‑139 (MQ=255) aaTGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TC‑GGGGGG‑CTGATCGGGGCTg > 1:777993/1‑139 (MQ=255) tGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TC‑GGGGGG‑CTGATCGGGGCTGgt < 2:777993/139‑1 (MQ=255) ttGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGgtg < 2:1234076/139‑1 (MQ=255) ggATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACccg > 1:516847/1‑139 (MQ=255) gATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TC‑GGGGGGCCTGATCGGGGCTGGTGTCGCATTAACccgc > 2:766640/1‑139 (MQ=255) cgcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAAcc > 1:84476/1‑132 (MQ=255) cgcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAAcc < 2:84476/132‑1 (MQ=255) gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTg > 1:1069538/1‑139 (MQ=255) cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc > 2:759488/1‑139 (MQ=255) cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc > 1:1296764/1‑139 (MQ=255) cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc > 1:552209/1‑139 (MQ=255) gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 1:253304/139‑1 (MQ=255) gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 2:516847/139‑1 (MQ=255) gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 1:616625/139‑1 (MQ=255) gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 1:766640/139‑1 (MQ=255) ttttCCGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCCGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc < 1:819087/139‑1 (MQ=255) ttCAGACCTTTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa < 2:902071/139‑1 (MQ=255) tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTTCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca > 1:1168937/1‑139 (MQ=255) tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca < 2:601751/139‑1 (MQ=255) tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca > 1:670900/1‑139 (MQ=255) cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGt > 2:741723/1‑139 (MQ=255) cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa < 2:1159230/115‑1 (MQ=255) cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa > 1:1159230/1‑115 (MQ=255) aagaaCCGGTAAAACGCGATAGCCCACGCCGTGCCTTCCTGATGATTATGCGGGGCATTGTTG‑GGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAg > 2:436546/1‑139 (MQ=255) aaCCGGGAAAACGCGATAGCCGACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTCCTGCGGTGAGCGCAAAGgcg < 2:771448/139‑1 (MQ=255) ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa > 1:1040399/1‑112 (MQ=255) ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa < 2:1040399/112‑1 (MQ=255) ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACActg < 1:137566/117‑1 (MQ=255) ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACActg > 2:137566/1‑117 (MQ=255) ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCt > 1:954555/1‑139 (MQ=255) cGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTc < 1:436546/139‑1 (MQ=255) aaaaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg < 2:643918/139‑1 (MQ=255) gcgATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAt < 2:1296764/139‑1 (MQ=255) aTAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCg > 2:1110304/1‑139 (MQ=255) cccACGTCGTGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAaga > 2:407989/1‑139 (MQ=255) cgtGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGTTCGGGGTTGGTGTCGCATTAACCCGCCGTTGCTCGAAAGAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa < 2:308496/139‑1 (MQ=255) cgtGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa < 2:954555/139‑1 (MQ=255) cgtGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa < 1:1110304/139‑1 (MQ=255) cgtGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa < 1:1138521/139‑1 (MQ=255) cgtGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa < 2:1069538/139‑1 (MQ=255) tGCCTTCCTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg > 2:542137/1‑139 (MQ=255) ccTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaa < 1:797932/77‑2 (MQ=255) ccTGATGATTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaa > 2:797932/1‑76 (MQ=255) atgatTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGgtg > 1:1131055/1‑44 (MQ=255) atgatTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGgtg < 2:1131055/44‑1 (MQ=255) atgatTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTact < 2:670900/139‑1 (MQ=255) gatTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga < 2:1168937/139‑1 (MQ=255) atTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac < 2:187832/139‑1 (MQ=255) atTATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac < 1:542137/139‑1 (MQ=255) tATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAAt < 2:1191405/66‑1 (MQ=255) tATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAAt > 1:1191405/1‑66 (MQ=255) tATGCGGGGCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgactg > 2:272788/1‑139 (MQ=255) tgcggggCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa < 2:433672/58‑1 (MQ=255) tgcggggCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa > 1:433672/4‑61 (MQ=255) tgcggggCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTa > 1:44516/4‑139 (MQ=255) ggggCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCg < 1:298953/116‑1 (MQ=255) ggggCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCg > 2:298953/1‑116 (MQ=255) ggCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATc > 1:1261191/1‑113 (MQ=255) ggCATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATc < 2:1261191/113‑1 (MQ=255) cATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAt < 1:283076/99‑1 (MQ=255) cATTG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAt > 2:283076/1‑99 (MQ=255) ttG‑TCGGGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTAcg < 1:768237/139‑1 (MQ=255) | GGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTG‑TC‑GGGGGG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACG > NC_000913/3969888‑3970159 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |