Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	49,831	T→C	S3S (AGT→AGC)	folA →	dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	49,831	0	T	C	100.0%	192.5 / NA	53	S3S (AGT→AGC)	folA	dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/28);  total (25/28)

GAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCG  >  NC_000913/49704‑49953                                                                                                                                 |                                                                                                                           gagagaGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTgcggc                                                                                                                 <  2:916420/139‑1 (MQ=255)   gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                                                                               <  2:953302/139‑1 (MQ=255)   gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                                                                               <  1:255563/139‑1 (MQ=255)    agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGtt                                                                                                              >  1:725836/1‑139 (MQ=255)    agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGtt                                                                                                              >  2:1221306/1‑139 (MQ=255)      agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAg                                                                                                            >  1:425800/1‑139 (MQ=255)                 gCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                                                                               <  1:1090905/125‑1 (MQ=255)                 gCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                                                                               >  2:1090905/1‑125 (MQ=255)                      cagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTAt                                                                                            >  2:250530/1‑139 (MQ=255)                      cagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTAt                                                                                            <  1:1289353/139‑1 (MQ=255)                          atatAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGc                                                                                        <  1:1221306/139‑1 (MQ=255)                           tataAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCa                                                                                       <  2:56260/139‑1 (MQ=255)                                aaTTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGaa                                                                                  <  2:293826/139‑1 (MQ=255)                                       cTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCa                                                                           <  2:397984/139‑1 (MQ=255)                                        tCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCAt                                                                          <  2:676133/139‑1 (MQ=255)                                          aaCATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGc                                                                        >  1:750986/1‑139 (MQ=255)                                                 tcCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGaa                                                                 >  2:314138/1‑139 (MQ=255)                                                      gCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgc                                                            >  2:1123568/1‑139 (MQ=255)                                                             ttgacgacGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                                                                               >  1:543197/3‑81 (MQ=255)                                                             ttgacgacGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                                                                               <  2:543197/79‑1 (MQ=255)                                                              tgacgacGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAt                                                    <  1:607234/138‑1 (MQ=255)                                                              tgacgacGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAt                                                    <  2:249119/138‑1 (MQ=255)                                                                   acgGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGc                                               <  2:425800/139‑1 (MQ=255)                                                                    ataaggggaagttACCTATTGTGGGGGCAATTTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGc                                               <  2:1016991/128‑1 (MQ=255)                                                                      gTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTg                                                                                                                     >  2:960697/1‑66 (MQ=255)                                                                      gTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTg                                                                                                                     <  1:960697/66‑1 (MQ=255)                                                                      gTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTg                                            >  2:488203/1‑139 (MQ=255)                                                                      atttACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGCAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGTTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTg                                            >  1:809195/2‑139 (MQ=255)                                                                               ttACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGttt                                        <  1:405208/134‑1 (MQ=255)                                                                               ttACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGttt                                        >  2:405208/1‑134 (MQ=255)                                                                                tACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGc                                  >  1:948262/1‑139 (MQ=255)                                                                                 aCGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCa                                 >  1:1183384/1‑139 (MQ=255)                                                                                 aCGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCa                                 <  1:1123568/139‑1 (MQ=255)                                                                                 aCGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCa                                 <  2:750986/139‑1 (MQ=255)                                                                                 aCGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCa                                 <  2:725836/139‑1 (MQ=255)                                                                                  cGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa                                <  1:250530/139‑1 (MQ=255)                                                                                      taGTTGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGAGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTGGCCTGGTTTAAACGCAACAcc                            >  2:764315/1‑139 (MQ=255)                                                                                        gTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCtt                          >  2:1274446/1‑139 (MQ=255)                                                                                          ggCGACAATTTTTTTTTATCGGGAAATCTCAATGATCACCCTGATTGCGGCGTAAGGGTTAAATCCGGTATTCGGCAGGGAAAACCCCAGGCCTTGGACCCTGCCTGCCGACCTCCCCTGGTTTAAAccggtctcttat                         >  1:1016991/1‑128 (MQ=255)                                                                                          ggCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCgg                                                                                                         <  2:458914/58‑1 (MQ=255)                                                                                          ggCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCgg                                                                                                         >  1:458914/1‑58 (MQ=255)                                                                                            cGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                                                                               >  2:676077/1‑50 (MQ=255)                                                                                            cGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                                                                               <  1:1003891/50‑1 (MQ=255)                                                                                            cGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                                                                               <  1:676077/50‑1 (MQ=255)                                                                                            cGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                                                                               >  2:1003891/1‑50 (MQ=255)                                                                                              aCAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTa                                                                                             >  1:1179327/1‑66 (MQ=255)                                                                                              aCAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTa                                                                                             <  2:1179327/66‑1 (MQ=255)                                                                                              aCAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaataa                    >  2:524514/1‑139 (MQ=255)                                                                                                             cGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgcctg                                                         >  1:350591/1‑88 (MQ=255)                                                                                                             cGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgcctg                                                         <  2:350591/88‑1 (MQ=255)                                                                                                             cGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg      <  1:1274446/139‑1 (MQ=255)                                                                                                                 aaaTCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg  >  2:236381/1‑139 (MQ=255)                                                                                                                              cAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATg       <  2:1062377/121‑1 (MQ=255)                                                                                                                                 |                                                                                                                           GAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCG  >  NC_000913/49704‑49953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑