Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	247,461	G→A	*250* (TAG→TAA)	yafL →	putative lipoprotein and C40 family peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	247,461	0	G	A	100.0%	106.3 / NA	34	*250* (TAG→TAA)	yafL	putative lipoprotein and C40 family peptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/15);  total (19/15)

TGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCC  >  NC_000913/247325‑247594                                                                                                                                         |                                                                                                                                      tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGa                                                                                                                                     >  1:899591/1‑139 (MQ=255) tgtATTTGGGCGATGGGAAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGa                                                                                                                                     >  2:1020993/1‑139 (MQ=255)  gtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGTGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAc                                                                                                                                    >  2:428294/1‑139 (MQ=255)             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGtt                                                                                                                        >  1:1022854/1‑139 (MQ=255)             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGtt                                                                                                                        >  2:269808/1‑139 (MQ=255)             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGtt                                                                                                                        >  1:26695/1‑139 (MQ=255)              tGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGttt                                                                                                                       >  1:155283/1‑139 (MQ=255)                  cAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTAcgc                                                                                                                   <  2:155283/139‑1 (MQ=255)                         tCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATcc                                                                                                            <  1:1139586/139‑1 (MQ=255)                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCGTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  1:683413/139‑1 (MQ=255)                              tCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                       <  1:1201564/139‑1 (MQ=255)                                gCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGtt                                                                                                     >  1:55343/1‑139 (MQ=255)                                               aaCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTg                                                                                      >  2:312017/1‑139 (MQ=255)                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  2:615395/139‑1 (MQ=255)                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  1:1006243/139‑1 (MQ=255)                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         >  1:467400/1‑139 (MQ=255)                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  1:553472/139‑1 (MQ=255)                                                                                   gCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  1:1300727/83‑1 (MQ=255)                                                                                   gCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCgg                                                                                                          >  2:1300727/1‑83 (MQ=255)                                                                                      ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAg                                                                                             >  2:1093774/1‑93 (MQ=255)                                                                                      ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAg                                                                                             <  1:1093774/93‑1 (MQ=255)                                                                                               tttGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTg                                      >  2:1036966/1‑139 (MQ=255)                                                                                                  gggCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGa                                   >  2:158795/1‑139 (MQ=255)                                                                                                   ggCGCGCGCAGGATTTTGACGGAAGAGACGATTTGGTAAGAC‑GATAAGGCGTGTACGCCGCATCCGGCAGTTGGACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAg                                  <  2:899591/139‑1 (MQ=37)                                                                                                       cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGc                              >  1:333653/1‑139 (MQ=255)                                                                                                       cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGACGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGc                              >  2:931303/1‑139 (MQ=255)                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  2:1022854/139‑1 (MQ=255)                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  1:312017/139‑1 (MQ=255)                                                                                                                  ttGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGatat                   <  2:333653/139‑1 (MQ=255)                                                                                                                   tGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGAtata                  >  2:154751/1‑139 (MQ=255)                                                                                                                          agagACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaacaac           <  1:154751/139‑1 (MQ=255)                                                                                                                                gATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCAtt     >  1:424639/1‑139 (MQ=255)                                                                                                                                  ttttGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGc   >  2:462174/1‑139 (MQ=255)                                                                                                                                   tttGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGcc  <  1:1281658/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      TGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCC  >  NC_000913/247325‑247594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑