Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,308,040	C→T	*295* (TAG→TAA)	nlpI ←	lipoprotein involved in osmotic sensitivity and filamentation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,308,040	0	C	T	100.0%	224.2 / NA	70	*295* (TAG→TAA)	nlpI	lipoprotein involved in osmotic sensitivity and filamentation
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/32);  total (38/32)

TCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCC  >  NC_000913/3307905‑3308175                                                                                                                                        |                                                                                                                                         tcatcaTGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTAt                                                                                                                                       >  2:835065/1‑139 (MQ=255) tcatcaTGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTAt                                                                                                                                       >  2:740348/1‑139 (MQ=255)   atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTg                                                                                                                                     >  1:566978/1‑139 (MQ=255)        gAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAACGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGt                                                                                                                                >  2:930352/1‑139 (MQ=255)               aTCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTc                                                                                                                         >  1:811641/1‑139 (MQ=255)                  aGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGc                                                                                                                      <  1:1066328/139‑1 (MQ=255)                      aTTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAgg                                                                                                                  <  1:70342/139‑1 (MQ=255)                         ttCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCa                                                                                                               >  1:257781/1‑139 (MQ=255)                            aTTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCt                                                                                                            <  2:415781/139‑1 (MQ=255)                            aTTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCt                                                                                                            <  1:930352/139‑1 (MQ=255)                                  aaGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                                      <  2:868567/139‑1 (MQ=255)                                  aaGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                                      >  1:850141/1‑139 (MQ=255)                                        gAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGccc                                                                                               <  1:1203908/139‑1 (MQ=255)                                              gCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGc                                                                                         >  2:1129177/1‑139 (MQ=255)                                               gtCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCGTGGTCCT‑GGCCCAGGAGCg                                                                                        <  2:816001/138‑1 (MQ=255)                                               cTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGGCCCAGGAGc                                                                                         >  1:557848/1‑139 (MQ=255)                                               cTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCg                                                                                        >  2:1276965/1‑139 (MQ=255)                                               cTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCg                                                                                        >  1:627584/1‑139 (MQ=255)                                               cTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCg                                                                                        <  2:144740/139‑1 (MQ=255)                                                 cAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGAt                                                                                      <  1:835065/139‑1 (MQ=255)                                                 cAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGAt                                                                                      <  2:983915/139‑1 (MQ=255)                                                        ggTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                                      >  2:1052199/1‑117 (MQ=255)                                                        ggTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                                      <  1:1052199/117‑1 (MQ=255)                                                        ggTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCca                                                                               >  1:911208/1‑139 (MQ=255)                                                         gtgtATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCcaa                                                                              <  1:740348/139‑1 (MQ=255)                                                           gtATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCcaaca                                                                            <  2:257781/139‑1 (MQ=255)                                                            tATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCcaacaa                                                                           >  2:177282/1‑139 (MQ=255)                                                              ttAACGAACAACAACGCCCTCACCCGTTAAGGTGATGACAATCAAAAAAGATTACGGGCCGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                                      >  2:1188424/1‑111 (MQ=255)                                                              ttAACGAACAACAACGCCCTCACCCGTTAAGGTGATGACAATCAAAAAAGATTACGGGCCGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                                      <  1:1188424/111‑1 (MQ=255)                                                                   gAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTAt                                                                    <  2:1236693/139‑1 (MQ=255)                                                                    aacaacaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATc                                                                   >  2:1086065/1‑139 (MQ=255)                                                                     acaacaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCg                                                                  <  1:177282/139‑1 (MQ=255)                                                                           acGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTAGTGCTGGTCCGATTCTGCGAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTc                                                            >  2:345318/1‑139 (MQ=255)                                                                             gCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTcaa                                                          >  1:456294/1‑139 (MQ=255)                                                                              cccTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGTTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTcaac                                                         >  1:312859/1‑139 (MQ=255)                                                                                       gTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATg                                                >  1:678752/1‑139 (MQ=255)                                                                                        ttAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGa                                               >  2:110929/1‑139 (MQ=255)                                                                                         tAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGaa                                              <  2:678752/139‑1 (MQ=255)                                                                                         tAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGaa                                              <  2:957250/139‑1 (MQ=255)                                                                                         tAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGaa                                              >  1:1027976/1‑139 (MQ=255)                                                                                         tAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGaa                                              >  2:1089251/1‑139 (MQ=255)                                                                                         tAAGGTGATGGCAATCAAAAAAAATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGaa                                              >  1:1256719/1‑139 (MQ=255)                                                                                               gATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCt                                                                                                            >  1:276239/1‑72 (MQ=255)                                                                                               gATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCt                                                                                                            <  2:276239/72‑1 (MQ=255)                                                                                               gATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTg                                                               >  1:709247/1‑116 (MQ=255)                                                                                               gATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTg                                                               <  2:709247/116‑1 (MQ=255)                                                                                               gATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACg                                            >  1:382832/1‑135 (MQ=255)                                                                                               gATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACg                                            <  2:382832/135‑1 (MQ=255)                                                                                                aTGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACgttgtt                                       >  2:429460/1‑139 (MQ=255)                                                                                                 tGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGttgttg                                      >  2:302607/1‑139 (MQ=255)                                                                                                   gCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTTCTCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTTCTCAACAAAGTTATGAACGTTGTTGGc                                    <  2:1027976/139‑1 (MQ=255)                                                                                                   gCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGc                                    <  1:558730/139‑1 (MQ=255)                                                                                                      aTCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAAc                                 >  2:848625/1‑139 (MQ=255)                                                                                                       tCAAAAAAGATTACGTGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAAcc                                >  2:879187/1‑139 (MQ=255)                                                                                                       tCAAAAAAGATTACGGGCTGATGTGTATGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAAcc                                >  1:488755/1‑139 (MQ=255)                                                                                                        cAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg                               <  1:1276965/139‑1 (MQ=255)                                                                                                        cAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg                               <  2:456294/139‑1 (MQ=255)                                                                                                        cAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg                               <  2:627584/139‑1 (MQ=255)                                                                                                        cAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg                               <  1:302607/139‑1 (MQ=255)                                                                                                                    cGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACa                   <  1:1129177/139‑1 (MQ=255)                                                                                                                     gggCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAg                  <  1:1089251/139‑1 (MQ=255)                                                                                                                         tGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGcc              >  2:730852/1‑139 (MQ=255)                                                                                                                          gATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCg             >  2:1090824/1‑139 (MQ=255)                                                                                                                            tgtgTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCg                                                                  >  1:1128051/1‑84 (MQ=255)                                                                                                                            tgtgTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCg                                                                  <  2:1128051/84‑1 (MQ=255)                                                                                                                                 aCGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGc                                    <  2:157871/109‑1 (MQ=255)                                                                                                                                 aCGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGc                                    >  1:157871/1‑109 (MQ=255)                                                                                                                                     cAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCg                                                                       <  1:726962/70‑1 (MQ=255)                                                                                                                                     cAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCg                                                                       >  2:726962/1‑70 (MQ=255)                                                                                                                                     cAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTcc  >  2:610969/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                         TCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCT‑GGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCC  >  NC_000913/3307905‑3308175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑