Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,385,857	C→T	*91* (TAG→TAA)	yhcO ←	putative barnase inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,385,857	0	C	T	100.0%	213.6 / NA	66	*91* (TAG→TAA)	yhcO	putative barnase inhibitor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/32);  total (34/32)

AGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGGCA  >  NC_000913/3385726‑3385990                                                                                                                                     |                                                                                                                                      aGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGAc                                                                                                                                >  1:1070239/1‑139 (MQ=255)    cgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGaa                                                                                                                             >  1:136953/1‑139 (MQ=255)       aaCGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACAt                                                                                                                          >  2:689665/1‑139 (MQ=255)       aaCGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACAt                                                                                                                          >  1:1171639/1‑139 (MQ=255)                   gATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAAt                                                                                                              <  1:490201/139‑1 (MQ=255)                     ttACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGc                                                                                                            <  1:779431/139‑1 (MQ=255)                           aaGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACg                                                                                                                               <  2:980507/114‑1 (MQ=255)                           aaGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACg                                                                                                                               >  1:980507/1‑114 (MQ=255)                           aaGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                                                      <  2:136953/139‑1 (MQ=255)                           aaGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                                                      <  1:961672/139‑1 (MQ=255)                           aaGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                                                      >  1:316878/1‑139 (MQ=255)                             gCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAg                                                                                                    >  1:610614/1‑139 (MQ=255)                               tCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                  <  2:316878/139‑1 (MQ=255)                                cGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGctc                                                                                                 <  2:1070239/139‑1 (MQ=255)                                cGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGctc                                                                                                 >  1:550962/1‑139 (MQ=255)                                     cGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCct                                                                                            <  1:240088/139‑1 (MQ=255)                                           cacCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGcc                                                                                       <  2:1194585/139‑1 (MQ=255)                                                  cACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                                                         >  2:408666/1‑129 (MQ=255)                                                  cACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                                                         <  1:408666/129‑1 (MQ=255)                                                  cACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaaa                                                                               <  1:582010/139‑1 (MQ=255)                                                  cACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGAAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaaa                                                                               <  2:925951/139‑1 (MQ=255)                                                     gCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                                            <  1:689665/139‑1 (MQ=255)                                                      cTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGc                                                                           >  2:243681/1‑139 (MQ=255)                                                       tACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCa                                                                          >  1:109496/1‑139 (MQ=255)                                                               aaCTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                                            <  2:1150679/129‑1 (MQ=255)                                                               aaCTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                                            >  1:1150679/1‑129 (MQ=255)                                                               aaCTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAAc                                                                  >  2:1043102/1‑139 (MQ=255)                                                                 cTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAAcgc                                                                >  1:561941/1‑139 (MQ=255)                                                                        aaTAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCaaaa                                                         <  1:243681/139‑1 (MQ=255)                                                                              cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaaa                                                                               <  2:1314443/111‑1 (MQ=255)                                                                              cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaaa                                                                               >  1:1314443/1‑111 (MQ=255)                                                                              cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGa                                                   >  2:166879/1‑139 (MQ=255)                                                                              cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGa                                                   >  1:186406/1‑139 (MQ=255)                                                                                 tCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAcgc                                                <  1:1043102/139‑1 (MQ=255)                                                                                 tCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAcgc                                                >  1:1194008/1‑139 (MQ=255)                                                                                        cTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtct                                         >  2:411240/1‑139 (MQ=255)                                                                                        cTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtct                                         >  2:1286094/1‑139 (MQ=255)                                                                                           gTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtctct                                       >  2:1248725/1‑139 (MQ=255)                                                                                           gTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCt                                                                                                         <  2:259156/72‑1 (MQ=255)                                                                                           gTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCt                                                                                                         >  1:259156/1‑72 (MQ=255)                                                                                            tCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTcc                                     <  1:641910/139‑1 (MQ=255)                                                                                               gacgaTATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaa                                                                                <  1:1047140/93‑1 (MQ=255)                                                                                               gacgaTATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaa                                                                                >  2:1047140/1‑93 (MQ=255)                                                                                                 cgaTATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtttt                                           <  2:1097477/128‑1 (MQ=255)                                                                                                 cgaTATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtttt                                           >  1:1097477/1‑128 (MQ=255)                                                                                                    tatTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGaa                             <  2:186406/139‑1 (MQ=255)                                                                                                         cccccGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACg                                                                                                                               <  1:707665/36‑1 (MQ=38)                                                                                                         cccccGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACg                                                                                                                               >  2:707665/1‑36 (MQ=255)                                                                                                             cGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAATACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAACCGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCaa                    <  1:878505/139‑1 (MQ=255)                                                                                                              ggTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                  >  1:1160044/1‑60 (MQ=255)                                                                                                              ggTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                  <  2:1160044/60‑1 (MQ=255)                                                                                                              ggTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGcc                                                                                       <  2:18087/71‑1 (MQ=255)                                                                                                              ggTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGcc                                                                                       >  1:18087/1‑71 (MQ=255)                                                                                                              ggTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtc                                          >  2:52212/1‑116 (MQ=255)                                                                                                              ggTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtc                                          <  1:52212/116‑1 (MQ=255)                                                                                                              ggTTCGGGGGC‑‑TTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                                                         >  2:1237860/1‑68 (MQ=255)                                                                                                              ggTTCGGGGGC‑‑TTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                                                         <  1:1237860/68‑1 (MQ=255)                                                                                                               gTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAtc                  <  1:166879/139‑1 (MQ=255)                                                                                                                   gggggC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCaa              >  1:323742/1‑139 (MQ=255)                                                                                                                    ggggC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAg             >  1:51871/1‑139 (MQ=255)                                                                                                                        c‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGt         <  2:561941/139‑1 (MQ=255)                                                                                                                         tttttttttGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGt         >  1:1088619/2‑139 (MQ=255)                                                                                                                           tttttttGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAat                                                                        <  2:1227669/74‑1 (MQ=255)                                                                                                                           tttttttGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAat                                                                        >  1:1227669/1‑74 (MQ=255)                                                                                                                           tttttttGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGTGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAg       >  1:156997/1‑139 (MQ=255)                                                                                                                                ttGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGGCa  <  1:1248725/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                      AGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGGCA  >  NC_000913/3385726‑3385990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑