Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,764,018	C→T	*123* (TAG→TAA)	sufA ←	Fe‑S cluster assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,764,018	0	C	T	100.0%	170.5 / NA	53	*123* (TAG→TAA)	sufA	Fe‑S cluster assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/26);  total (27/26)

ACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCAGCGG  >  NC_000913/1763882‑1764149                                                                                                                                         |                                                                                                                                    accaccTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTtat                                                                                                                                  <  1:1311315/139‑1 (MQ=255)                aTCCCCTTTGCCAGCTCATCAGTGGCTAACTCGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATTGTACCGCTTATACCCCGAAGCtttac                                                                                                                   >  1:948271/1‑137 (MQ=255)                   cccTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgcca                                                                                                                <  1:1130724/139‑1 (MQ=255)                   cccTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgcca                                                                                                                >  1:1473372/1‑139 (MQ=255)                       ttGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                                                            <  2:1473372/139‑1 (MQ=255)                             gCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgc                                                                                                                  <  2:186092/127‑1 (MQ=255)                             gCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgc                                                                                                                  >  1:186092/1‑127 (MQ=255)                              cTCATCGGTGGCTAACTGGGTGAAGAATACTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGATATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcattc                                                                                                     >  2:998975/1‑139 (MQ=255)                                aaTCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcattcat                                                                                                   >  1:596139/2‑139 (MQ=255)                                 aTCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcattcatt                                                                                                  <  2:596139/139‑1 (MQ=255)                                 aTCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcat                                                                                                       <  1:979592/134‑1 (MQ=255)                                 aTCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcat                                                                                                       >  2:979592/1‑134 (MQ=255)                                    ggTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                                                               <  1:14604/139‑1 (MQ=255)                                         cTAACTGGGTGAAGAATCCTTGTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCtt                                                                                          <  2:948271/139‑1 (MQ=255)                                                ggTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                                                            <  1:1330536/114‑1 (MQ=255)                                                ggTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                                                            >  2:1330536/1‑114 (MQ=255)                                                    aagaaTCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGa                                                                               >  1:1209466/1‑139 (MQ=255)                                                            ttcttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                                                               <  1:1254563/115‑1 (MQ=255)                                                            ttcttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                                                               >  2:1254563/1‑115 (MQ=255)                                                               tttATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAatat                                                                    <  1:998975/139‑1 (MQ=255)                                                                 tataATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCt                                                                  >  1:827909/1‑139 (MQ=255)                                                                   taATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAAt                                                                            >  1:1475404/1‑127 (MQ=255)                                                                   taATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAAt                                                                            <  2:1475404/127‑1 (MQ=255)                                                                         aGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcat                                                                                                       >  2:1260814/1‑94 (MQ=255)                                                                         aGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcat                                                                                                       <  1:1260814/94‑1 (MQ=255)                                                                         aGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTACTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa                                                                        <  2:805621/125‑1 (MQ=255)                                                                         aGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTACTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa                                                                        >  1:805621/1‑125 (MQ=255)                                                                           cGGGCCGCCGGTCCAGGTTTTGACATCGTGA‑TTGCTTCAGTATTAAGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTAGTGAAATTTGAATATCTGATTAAGTcc                                                        >  1:929529/1‑139 (MQ=255)                                                                           cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCa                                                                                                                            >  1:580277/1‑71 (MQ=255)                                                                           cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCa                                                                                                                            <  2:580277/71‑1 (MQ=255)                                                                           cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                                                            >  1:880241/1‑87 (MQ=255)                                                                           cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                                                            <  2:880241/87‑1 (MQ=255)                                                                           cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                                                                                <  2:1612510/99‑1 (MQ=255)                                                                           cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                                                                                >  1:1612510/1‑99 (MQ=255)                                                                           cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa                                                                        >  2:413310/1‑123 (MQ=255)                                                                           cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa                                                                        <  1:413310/123‑1 (MQ=255)                                                                           cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTcc                                                        >  2:713901/1‑139 (MQ=255)                                                                                cgccgGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTa                                                                                        <  2:1045744/102‑1 (MQ=255)                                                                                cgccgGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCac                                                   >  2:725322/1‑139 (MQ=255)                                                                                cgccgGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGAGCTTTa                                                                                        >  1:1045744/1‑102 (MQ=255)                                                                                               tGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACtt                                    >  1:548142/1‑139 (MQ=255)                                                                                                      cgtcattGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                                                                                <  2:760020/68‑1 (MQ=255)                                                                                                      cgtcattGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                                                                                >  1:760020/6‑73 (MQ=255)                                                                                                      cgtcattGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTCCACGAACGAAATCGACTTCCGTGc                              <  2:983948/134‑1 (MQ=255)                                                                                                          attGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                                                                                >  2:515608/2‑69 (MQ=255)                                                                                                          attGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCt                                                                                                <  1:515608/68‑1 (MQ=255)                                                                                                                  aGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTAACGAACGAAATCGCCTTCCGTGCCATCAATAAAct                  >  2:947336/1‑138 (MQ=255)                                                                                                                   gTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCAt                           <  2:1038551/129‑1 (MQ=255)                                                                                                                   gTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCAt                           >  1:1038551/1‑129 (MQ=255)                                                                                                                      ttACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                                                               >  2:1550207/1‑58 (MQ=255)                                                                                                                      ttACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                                                               <  1:1550207/58‑1 (MQ=255)                                                                                                                              cATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCa      <  1:713901/139‑1 (MQ=255)                                                                                                                                  gTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCAGCgg  <  2:1209466/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    ACCACCTCTTCGTTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCAGCGG  >  NC_000913/1763882‑1764149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑