BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,032,032	G→A	S222S (TCG→TCA)	uacT →	uric acid permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,032,032	0	G	A	99.0%	328.9 / NA	101	S222S (TCG→TCA)	uacT	uric acid permease
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (57/43); minor base T (0/1); total (57/44)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

CCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAATC  >  NC_000913/3031911‑3032161

ccGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATgg                                                                                                                                 >  1:67315/1‑125 (MQ=255)

ccGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATgg                                                                                                                                 <  2:67315/125‑1 (MQ=255)

  gTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATgg                                                                                                                                 <  1:1084950/123‑1 (MQ=255)

  gTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCa                                                                                                                 >  1:1595242/1‑139 (MQ=255)

  gTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCa                                                                                                                 >  1:559803/1‑139 (MQ=255)

  gTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCa                                                                                                                 >  2:747320/1‑139 (MQ=255)

  gTTTATTTAGGAATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCa                                                                                                                 >  2:207535/1‑139 (MQ=255)

   tttatttaGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGatgaa                                                                                                                         >  1:1426737/1‑130 (MQ=255)

   tttatttaGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGatgaa                                                                                                                         <  2:1426737/130‑1 (MQ=255)

    ttatttaGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATAACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAAt                                                                                                               >  2:1065339/1‑139 (MQ=255)

         taGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATc                                                                                                          <  1:1197157/139‑1 (MQ=255)

          aGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATcc                                                                                                         >  2:1302834/1‑139 (MQ=255)

            gTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCgg                                                                                                       <  1:596145/139‑1 (MQ=255)

               tctcCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCt                                                                                                    >  2:768827/1‑139 (MQ=255)

               tctcCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCt                                                                                                    >  1:1485236/1‑139 (MQ=255)

               tctcCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCt                                                                                                    >  1:105712/1‑139 (MQ=255)

                ctcCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTa                                                                                                   >  1:49950/1‑139 (MQ=255)

                 tcCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGatgaat                                                                                                                        <  1:1412064/117‑1 (MQ=255)

                 tcCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGatgaat                                                                                                                        >  2:1412064/1‑117 (MQ=255)

                 tcCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTAc                                                                                                  >  2:1336228/1‑139 (MQ=255)

                    tttGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACa‑tg                                                                                              <  2:1130062/139‑1 (MQ=255)

                      tGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACa‑tgat                                                                                            >  2:1250781/1‑139 (MQ=255)

                      tGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACa‑tgat                                                                                            >  2:188524/1‑139 (MQ=255)

                           tCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCggg                                                                                                      <  2:87725/125‑1 (MQ=255)

                           tCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCggg                                                                                                      >  1:87725/1‑125 (MQ=255)

                           tCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTc                                                                                       >  1:726683/1‑139 (MQ=255)

                           tCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTc                                                                                       >  1:408703/1‑139 (MQ=255)

                            cTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCa                                                                                      <  1:188524/139‑1 (MQ=255)

                             ttAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGgatga                                                                                                                             >  2:151193/1‑100 (MQ=255)

                             ttAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGgatga                                                                                                                             <  1:151193/100‑1 (MQ=255)

                             ttAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCa                                                                                      >  2:510555/1‑138 (MQ=255)

                             ttAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCa                                                                                      <  1:510555/138‑1 (MQ=255)

                             ttAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCAt                                                                                     >  2:720750/1‑139 (MQ=255)

                                     tATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACa‑tg                                                                                              <  1:1324409/122‑1 (MQ=255)

                                     tATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACa‑tg                                                                                              >  2:1324409/1‑122 (MQ=255)

                                     tATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCg                                                                             <  1:1336228/139‑1 (MQ=255)

                                      aTCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGa                                                                            <  1:1607546/139‑1 (MQ=255)

                                         ttGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCgg                                                                                                       <  1:313959/110‑1 (MQ=255)

                                         ttGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCgg                                                                                                       >  2:313959/1‑110 (MQ=255)

                                            cTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTa                                                                      >  1:405979/1‑139 (MQ=255)

                                            cTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTa                                                                      <  1:720750/139‑1 (MQ=255)

                                                 tACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGCATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCg                                                                 <  1:1065339/139‑1 (MQ=255)

                                                 tACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCg                                                                 <  2:906481/139‑1 (MQ=255)

                                                      gCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTc                                                            >  2:650756/1‑139 (MQ=255)

                                                       cTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCg                                                           >  2:344327/1‑139 (MQ=255)

                                                         aTGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGgatga                                                                                                                             >  2:870186/1‑72 (MQ=255)

                                                         aTGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGgatga                                                                                                                             <  1:870186/72‑1 (MQ=255)

                                                           gCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCTGGGCTACA‑TGATGCTTCATGGTTTGCGATTGt                                                                        <  1:601650/122‑1 (MQ=255)

                                                           gCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCTGGGCTACA‑TGATGCTTCATGGTTTGCGATTGt                                                                        >  2:601650/1‑122 (MQ=255)

                                                           gCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTg                                                       >  1:1277423/1‑139 (MQ=255)

                                                           gCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTg                                                       >  2:733254/1‑139 (MQ=255)

                                                             gAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGt                                                     <  2:105712/139‑1 (MQ=255)

                                                                         gTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTc                                         >  2:161292/1‑139 (MQ=255)

                                                                          tCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCg                                        <  1:747320/139‑1 (MQ=255)

                                                                           ccAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGa                                       >  2:324755/1‑139 (MQ=255)

                                                                            cAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCggg                                                                                                      <  1:983563/76‑1 (MQ=255)

                                                                             aaCGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATc                                     >  1:189489/1‑139 (MQ=255)

                                                                             aaCGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATc                                     >  2:396982/1‑139 (MQ=255)

                                                                              aCGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATcc                                    <  2:189489/139‑1 (MQ=255)

                                                                              aCGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATcc                                    <  1:768827/139‑1 (MQ=255)

                                                                                    ccGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTAt                                                                                                           >  2:1113284/1‑63 (MQ=255)

                                                                                    ccGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTAt                                                                                                           <  1:1113284/63‑1 (MQ=255)

                                                                                               gggATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTAt                                                                                                           <  1:1532045/52‑1 (MQ=255)

                                                                                               gggATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTAt                                                                                                           >  2:1532045/1‑52 (MQ=255)

                                                                                               gggATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCg                                                                                                        <  2:70119/55‑1 (MQ=255)

                                                                                               gggATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCg                                                                                                        >  1:70119/1‑55 (MQ=255)

                                                                                               gggATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATcc                                    <  2:370456/122‑1 (MQ=255)

                                                                                               gggATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATcc                                    >  1:370456/1‑122 (MQ=255)

                                                                                               gggATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCa                   >  1:228461/1‑139 (MQ=255)

                                                                                               gggATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCa                   >  2:362956/1‑139 (MQ=255)

                                                                                                ggATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCAt                  <  2:408703/139‑1 (MQ=255)

                                                                                                 gATTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATg                 >  2:923509/1‑139 (MQ=255)

                                                                                                  aTTGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGa                <  1:161292/139‑1 (MQ=255)

                                                                                                   ttGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATcc                                    <  1:246569/118‑1 (MQ=255)

                                                                                                   ttGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATcc                                    >  2:246569/1‑118 (MQ=255)

                                                                                                    tGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCg                                                                 >  2:980798/1‑88 (MQ=255)

                                                                                                    tGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCg                                                                 <  1:980798/88‑1 (MQ=255)

                                                                                                    tGTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACt              >  1:1286699/1‑139 (MQ=255)

                                                                                                     gTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTg             <  2:559803/139‑1 (MQ=255)

                                                                                                     gTATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTg             <  1:923509/139‑1 (MQ=255)

                                                                                                      tATTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGc            >  1:92981/1‑139 (MQ=255)

                                                                                                       ttttGGCTTTTTTACTTCATTGGTGGAT‑AATGAAATCAATTTAACCCGGCCACATTGAGCCTTAATGTTTTG‑GATTTTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGcc           <  1:1250781/138‑1 (MQ=255)

                                                                                                       aTTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGcc           >  1:1214798/1‑139 (MQ=255)

                                                                                                       aTTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGcc           <  1:650756/139‑1 (MQ=255)

                                                                                                       aTTTGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGcc           <  1:1302834/139‑1 (MQ=255)

                                                                                                         ttGGC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCgt         <  1:362956/139‑1 (MQ=255)

                                                                                                           ggC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTAc                                                                     <  2:242829/77‑1 (MQ=255)

                                                                                                           ggC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTAc                                                                     >  1:242829/1‑77 (MQ=255)

                                                                                                           ggC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCgtgt       <  1:733254/139‑1 (MQ=255)

                                                                                                           ggC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCgtgt       >  1:1477921/1‑139 (MQ=255)

                                                                                                           ggC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCgtgt       >  1:574757/1‑139 (MQ=255)

                                                                                                           ggC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCgtgt       <  2:92981/139‑1 (MQ=255)

                                                                                                           ggC‑TTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCgtgt       >  1:228656/1‑139 (MQ=255)

                                                                                                             c‑TTTTTACTTTCATGTATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTGGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTa     >  1:1075532/1‑139 (MQ=255)

                                                                                                                 tttACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACa‑tg                                                                                              >  1:1492365/1‑47 (MQ=255)

                                                                                                                 tttACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACa‑tg                                                                                              <  2:1492365/47‑1 (MQ=255)

                                                                                                                 tttACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAatc  >  2:1185541/1‑139 (MQ=255)

                                                                                                                 tttACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAatc  >  1:1363849/1‑139 (MQ=255)

                                                                                                                 tttACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAatc  >  1:516856/1‑139 (MQ=255)

                                                                                                                 tttACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAatc  <  1:344327/139‑1 (MQ=255)

CCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGC‑TTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACA‑TGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAATC  >  NC_000913/3031911‑3032161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`