Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,059,293	T→C	G11G (GGA→GGG)	rpiA ←	ribose 5‑phosphate isomerase, constitutive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,059,293	0	T	C	100.0%	336.2 / NA	93	G11G (GGA→GGG)	rpiA	ribose 5‑phosphate isomerase, constitutive
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/48);  total (45/48)

ATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATAAAATATC  >  NC_000913/3059164‑3059427                                                                                                                                  |                                                                                                                                        aTCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑t                                                                                                                               <  2:442998/139‑1 (MQ=255) aTCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑t                                                                                                                               <  1:147585/139‑1 (MQ=255)  tCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑tt                                                                                                                              >  2:141207/1‑139 (MQ=255)                ggCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATcc                                                                                                                >  1:874586/1‑139 (MQ=255)                       tCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCa                                                                                                         <  1:141207/139‑1 (MQ=255)                                ccTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGttt                                                                                                >  2:455618/1‑139 (MQ=255)                                     cATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑t                                                                                                                               <  1:1004140/103‑1 (MQ=255)                                     cATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑t                                                                                                                               >  2:1004140/1‑103 (MQ=255)                                         gTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTgg                                                                                       >  2:865439/1‑139 (MQ=255)                                         gTACCGAGCGCGTCAATAAAGTATGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTgg                                                                                       >  1:563122/1‑139 (MQ=255)                                               aGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGaa                                                                                 >  2:160498/1‑139 (MQ=255)                                               aGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGaa                                                                                 <  1:563400/139‑1 (MQ=255)                                               aGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGaa                                                                                 >  1:548060/1‑139 (MQ=255)                                               aGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGaa                                                                                 >  1:710860/1‑139 (MQ=255)                                               aGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGaa                                                                                 >  1:361480/1‑139 (MQ=255)                                               aGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGaa                                                                                 >  1:770480/1‑139 (MQ=255)                                               aGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGaa                                                                                 >  2:94246/1‑139 (MQ=255)                                               aGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGaa                                                                                 >  2:1391484/1‑139 (MQ=255)                                               aGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTATGGTATGaa                                                                                 >  1:949162/1‑139 (MQ=255)                                                gcgcGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                          <  1:1532341/129‑1 (MQ=255)                                                gcgcGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                          >  2:1532341/1‑129 (MQ=255)                                                    gTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTc                                                                            >  1:372804/1‑139 (MQ=255)                                                      cAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGGCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcac                                                                          <  2:880020/139‑1 (MQ=255)                                                      cAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcac                                                                          <  1:94246/139‑1 (MQ=255)                                                       aaTAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcaca                                                                         <  1:1391484/139‑1 (MQ=255)                                                        aTAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                        <  2:710860/139‑1 (MQ=255)                                                        aTAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                        <  1:1443521/139‑1 (MQ=255)                                                        aTAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                        <  2:361480/139‑1 (MQ=255)                                                        aTAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                        <  2:548060/139‑1 (MQ=255)                                                        aTAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                        <  1:1284421/139‑1 (MQ=255)                                                        aTAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                        <  2:949162/139‑1 (MQ=255)                                                        aTAAAGTATGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                        <  2:563122/139‑1 (MQ=255)                                                           aaGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCa                                                                     <  2:46971/139‑1 (MQ=255)                                                           aaGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCa                                                                     <  2:874586/139‑1 (MQ=255)                                                           aaGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCa                                                                     <  1:865439/139‑1 (MQ=255)                                                               gtgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATtat                                                                 >  2:179139/1‑139 (MQ=255)                                                                tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑t                                                                                                                               >  2:1429647/1‑76 (MQ=255)                                                                tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑t                                                                                                                               <  1:1429647/76‑1 (MQ=255)                                                                 gcggcgGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                        <  1:1162283/130‑1 (MQ=255)                                                                 gcggcgGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                        >  2:1162283/1‑130 (MQ=255)                                                                  cggcggTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCAt                                                                    >  1:1160098/1‑133 (MQ=255)                                                                  cggcggTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCAt                                                                    <  2:1160098/133‑1 (MQ=255)                                                                   ggcggTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑t                                                                                                                               <  2:159502/73‑1 (MQ=255)                                                                   ggcggTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑t                                                                                                                               >  1:159502/1‑73 (MQ=255)                                                                   ggcggTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCt                                                                                           <  2:706126/109‑1 (MQ=255)                                                                   ggcggTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCt                                                                                           >  1:706126/1‑109 (MQ=255)                                                                                  aCCTACACCAACAATGGTGTCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCa                                              >  1:1482389/1‑139 (MQ=255)                                                                                    cTACACCAACAATGGTGTCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGa                                            <  2:1482389/139‑1 (MQ=255)                                                                                      acacCAACAATGGTGCCGGGCTGAACATACCGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGAGCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTAAGAcc                                          >  1:963181/1‑139 (MQ=255)                                                                                         ccAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcac                                                                          <  2:938653/104‑1 (MQ=255)                                                                                         ccAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcac                                                                          >  1:938653/1‑104 (MQ=255)                                                                                           aacaaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                        >  1:1164408/1‑104 (MQ=255)                                                                                           aacaaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                        <  2:1164408/104‑1 (MQ=255)                                                                                            acaaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGc                                                                                             <  1:94863/83‑1 (MQ=255)                                                                                            acaaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGc                                                                                             >  2:94863/1‑83 (MQ=255)                                                                                             caaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                          >  2:871894/1‑84 (MQ=255)                                                                                             caaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                          <  1:871894/84‑1 (MQ=255)                                                                                             caaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTgg                                   >  2:165862/1‑139 (MQ=255)                                                                                                  gTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCt                                                                                                               <  1:211598/58‑1 (MQ=255)                                                                                                  gTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCt                                                                                                               >  2:211598/1‑58 (MQ=255)                                                                                                  gTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCt                                                                                           >  1:801953/1‑78 (MQ=255)                                                                                                  gTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCt                                                                                           <  2:801953/78‑1 (MQ=255)                                                                                                  gTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCa                                                                     <  2:61113/100‑1 (MQ=255)                                                                                                  gTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCa                                                                     >  1:61113/1‑100 (MQ=255)                                                                                                   tGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                        <  1:102633/96‑1 (MQ=255)                                                                                                   tGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                        >  2:102633/1‑96 (MQ=255)                                                                                                    gCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGAtt                                                >  1:923520/1‑119 (MQ=255)                                                                                                    gCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGAtt                                                <  2:923520/119‑1 (MQ=255)                                                                                                     ccGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCcgc                           <  1:179139/139‑1 (MQ=255)                                                                                                      cGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCcgcg                          >  2:825780/1‑139 (MQ=255)                                                                                                          cTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCt                                                                                           <  2:873456/70‑1 (MQ=255)                                                                                                          cTGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCt                                                                                           >  1:873456/1‑70 (MQ=255)                                                                                                           tGAACATACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGtt                     <  1:160498/139‑1 (MQ=255)                                                                                                                aTACTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTc                <  1:366357/139‑1 (MQ=255)                                                                                                                  aCTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCt                                                                                                               >  2:97537/1‑42 (MQ=255)                                                                                                                  aCTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCt                                                                                                               <  1:97537/42‑1 (MQ=255)                                                                                                                  aCTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCAt              <  2:770480/139‑1 (MQ=255)                                                                                                                  aCTGAAGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCAt              <  1:455618/139‑1 (MQ=255)                                                                                                                       aGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCcg                            >  1:1156593/1‑120 (MQ=255)                                                                                                                       aGTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCcg                            <  2:1156593/120‑1 (MQ=255)                                                                                                                        gTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcac                                                                          <  2:1052467/73‑1 (MQ=255)                                                                                                                        gTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATa         <  2:1393040/138‑1 (MQ=255)                                                                                                                        gTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATa         >  1:1393040/1‑138 (MQ=255)                                                                                                                        gTGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTAGGAAATTTcac                                                                          >  1:1052467/1‑73 (MQ=255)                                                                                                                         tGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                          <  2:435443/56‑1 (MQ=255)                                                                                                                         tGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                          >  1:435443/1‑56 (MQ=255)                                                                                                                         tGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcac                                                                          <  2:557579/72‑1 (MQ=255)                                                                                                                         tGCCGCCCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcac                                                                          >  1:557579/1‑72 (MQ=255)                                                                                                                          gccgccCACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAg                                                     <  2:968411/92‑1 (MQ=255)                                                                                                                          gacgccCACCCTACTGC‑TTTTGTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAATAg                                                     >  1:968411/3‑92 (MQ=255)                                                                                                                              cccACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATAAAATATc  >  2:613593/1‑139 (MQ=255)                                                                                                                              cccACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTAGGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATa         >  1:1425438/1‑132 (MQ=255)                                                                                                                              cccACCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTAGGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATa         <  2:1425438/132‑1 (MQ=255)                                                                                                                                  |                                                                                                                                        ATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGC‑TTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATAAAATATC  >  NC_000913/3059164‑3059427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑