Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,366,953	G→A	G10R (GGA→AGA)	yhcF →	putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,366,953	0	G	A	100.0%	301.5 / NA	91	G10R (GGA→AGA)	yhcF	putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (47/44);  total (47/44)

AGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTC  >  NC_000913/3366822‑3367087                                                                                                                                    |                                                                                                                                        aGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGc                                                                                                                                  <  2:906160/139‑1 (MQ=255)       aGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACAGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑ttttt                                                                                                                           >  2:356927/1‑139 (MQ=255)          cTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTgt                                                                                                                        <  1:1159410/139‑2 (MQ=255)          cTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGc                                                                                                                        <  1:370502/139‑1 (MQ=255)             acacTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCAt                                                                                                                     >  1:1561111/1‑139 (MQ=255)                   aCAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTTGCCATGTCAg                                                                                                                >  1:850365/1‑139 (MQ=255)                   aCAATGGCCCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAgc                                                                                                               >  2:872099/1‑139 (MQ=255)                       tGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGACAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCaa                                                                                                           >  1:1318475/1‑139 (MQ=255)                         gCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGc                                                                                                         >  2:561850/1‑139 (MQ=255)                           tCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCg                                                                                                       <  2:1152824/139‑1 (MQ=255)                               gCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGa                                                                                                   <  2:237883/139‑1 (MQ=255)                                cTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGACAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGAt                                                                                                  <  2:1318475/139‑1 (MQ=255)                                     aaGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGt                                                                                             <  1:561850/139‑1 (MQ=255)                                     aaGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGt                                                                                             <  1:1576411/139‑1 (MQ=255)                                          tGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCaa                                                                                        >  1:979536/1‑139 (MQ=255)                                           gAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAAt                                                                                       >  1:1579212/1‑139 (MQ=255)                                             aGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTg                                                                                     <  1:356927/139‑1 (MQ=255)                                                 gAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGt                                                                                 <  2:657851/139‑1 (MQ=255)                                                      aaCCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAgg                                                                            <  2:1561111/139‑1 (MQ=255)                                                       aCCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGt                                                                           >  1:64552/1‑139 (MQ=255)                                                         cGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGa                                                                         >  1:661410/1‑139 (MQ=255)                                                                 cAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGaa                                                                 >  2:46735/1‑139 (MQ=255)                                                                      atTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGc                                                            <  2:772572/139‑1 (MQ=255)                                                                         aCAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCaa                                                                                                           <  2:1216055/89‑1 (MQ=255)                                                                         aCAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCaa                                                                                                           >  1:1216055/1‑89 (MQ=255)                                                                         aCAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCt                                                              >  2:267296/1‑134 (MQ=255)                                                                         aCAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCt                                                              <  1:267296/134‑1 (MQ=255)                                                                          cAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCt                                                        >  1:1102214/1‑139 (MQ=255)                                                                           aGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCt                                                                                                     >  1:854741/1‑93 (MQ=255)                                                                           aGGCAATCTCACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCt                                                                                                     <  2:854741/93‑1 (MQ=255)                                                                               aaTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAAt                                                                                       <  1:321126/103‑1 (MQ=255)                                                                               aaTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAAt                                                                                       >  2:321126/1‑103 (MQ=255)                                                                                aTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGa                                                                                                   >  2:284650/1‑90 (MQ=255)                                                                                aTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGa                                                                                                   >  2:1610237/1‑90 (MQ=255)                                                                                aTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGa                                                                                                   <  1:284650/90‑1 (MQ=255)                                                                                aTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGa                                                                                                   <  1:1610237/90‑1 (MQ=255)                                                                                aTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACt                                                  >  2:1609956/1‑139 (MQ=255)                                                                                aTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACt                                                  >  1:315211/1‑139 (MQ=255)                                                                                 tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGc                                                                                                         >  1:1116447/1‑83 (MQ=255)                                                                                 tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGc                                                                                                         <  2:1116447/83‑1 (MQ=255)                                                                                 tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTc                                                 <  2:315211/139‑1 (MQ=255)                                                                                       ggTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGTC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGAt                                                                                                  <  1:242706/84‑1 (MQ=255)                                                                                       ggTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGAt                                                                                                  >  2:242706/1‑84 (MQ=255)                                                                                           cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGc                                                                                                                                  <  1:391812/49‑1 (MQ=255)                                                                                           cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGc                                                                                                                                  >  2:391812/1‑49 (MQ=255)                                                                                             ggtggAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGa                                                                                                   >  2:396849/1‑77 (MQ=255)                                                                                             ggtggAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGa                                                                                                   <  1:396849/77‑1 (MQ=255)                                                                                                   ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCaa                                                                                                           >  1:1158359/1‑63 (MQ=255)                                                                                                   ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCaa                                                                                                           <  2:1158359/63‑1 (MQ=255)                                                                                                   ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGc                                                                                                         >  1:1418938/1‑65 (MQ=255)                                                                                                   ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGc                                                                                                         <  2:1418938/65‑1 (MQ=255)                                                                                                   ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTa                                                    <  2:394968/118‑1 (MQ=255)                                                                                                   ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTa                                                    >  1:394968/1‑118 (MQ=255)                                                                                                   ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcggcg                               <  1:46735/139‑1 (MQ=255)                                                                                                   ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcggcg                               >  1:1216715/1‑139 (MQ=255)                                                                                                     cAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGa                             <  2:1191485/139‑1 (MQ=255)                                                                                                      aaaaTGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAg                            <  2:661410/139‑1 (MQ=255)                                                                                                        aaTGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAgtt                          >  2:552034/1‑139 (MQ=255)                                                                                                         aTGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGc                                                                                                         <  2:893400/59‑1 (MQ=255)                                                                                                         aTGAACAATGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGc                                                                                                         >  1:893400/1‑59 (MQ=255)                                                                                                                aTGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGc                                                                                                         <  2:298817/52‑1 (MQ=255)                                                                                                                aTGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGc                                                                                                         >  1:298817/1‑52 (MQ=255)                                                                                                                aTGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACtt                  >  2:204357/1‑139 (MQ=255)                                                                                                                aTGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACtt                  >  2:1612329/1‑139 (MQ=255)                                                                                                                 tGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACtt                  <  2:850365/139‑1 (MQ=255)                                                                                                                 tGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTc                 <  1:552034/139‑1 (MQ=255)                                                                                                                 tGTAAAATTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTc                 >  1:1267766/1‑139 (MQ=255)                                                                                                                    aaaaTTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCg                                                                                                       <  1:438046/50‑1 (MQ=255)                                                                                                                    aaaaTTACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCg                                                                                                       >  2:438046/1‑50 (MQ=255)                                                                                                                         tACTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCa         >  1:1374646/1‑139 (MQ=255)                                                                                                                           cTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATa                                                                                                 <  2:1585068/49‑1 (MQ=255)                                                                                                                           cTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATa                                                                                                 >  1:1585068/1‑49 (MQ=255)                                                                                                                           cTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGt                                                                                 >  1:1110725/1‑65 (MQ=255)                                                                                                                           cTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGt                                                                                 <  2:1110725/65‑1 (MQ=255)                                                                                                                           cTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGc                                      >  2:1365573/1‑108 (MQ=255)                                                                                                                           cTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGc                                      <  1:1365573/108‑1 (MQ=255)                                                                                                                           cTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCAtt       >  1:1487043/1‑139 (MQ=255)                                                                                                                           cTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCAtt       >  1:1033093/1‑139 (MQ=255)                                                                                                                           cTGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCAtt       <  1:23137/139‑1 (MQ=255)                                                                                                                            tGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGc                                                                                                         <  2:528409/40‑1 (MQ=255)                                                                                                                            tGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGc                                                                                                         >  1:528409/1‑40 (MQ=255)                                                                                                                            tGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTc      <  2:1374646/139‑1 (MQ=255)                                                                                                                            tGATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTc      <  1:872099/139‑1 (MQ=255)                                                                                                                             gATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTc                                                 <  2:1072745/95‑1 (MQ=255)                                                                                                                             gATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTc                                                 >  1:1072745/1‑95 (MQ=255)                                                                                                                             gATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCa     >  1:281435/1‑139 (MQ=255)                                                                                                                             gATTGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTCAACTTCGGTAGTCATTCa     >  2:1084342/1‑139 (MQ=255)                                                                                                                                tGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCaa                                              <  2:1502990/95‑1 (MQ=255)                                                                                                                                tGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCaa                                              >  1:1502990/1‑95 (MQ=255)                                                                                                                                tGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTc  <  2:1579212/139‑1 (MQ=255)                                                                                                                                tGCCAGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTc  >  2:770901/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                        AGCGTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCC‑TTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTC  >  NC_000913/3366822‑3367087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑