Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,860,416	G→A	*219* (TAG→TAA)	pphB →	serine/threonine‑specific protein phosphatase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,860,416	0	G	A	100.0%	154.5 / NA	48	*219* (TAG→TAA)	pphB	serine/threonine‑specific protein phosphatase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/23);  total (25/23)

AACAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATA  >  NC_000913/2860283‑2860551                                                                                                                                      |                                                                                                                                        aacaaATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCa                                                                                                                                    <  1:93591/139‑1 (MQ=255)  acaaATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCAt                                                                                                                                   >  2:113987/1‑139 (MQ=255)   caaATAAACGGCGCTGATTATTTTATATTGGGACATATGTTGTTTGATAACATTCAGACGTTCGCTAACAAGAATTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATg                                                                                                                                  <  1:328263/139‑1 (MQ=255)     aaTAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCa                                                                                                                                    >  2:71043/1‑135 (MQ=255)     aaTAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCa                                                                                                                                    <  1:71043/135‑1 (MQ=255)              cgcTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGa                                                                                                                      >  1:59231/1‑139 (MQ=255)                cTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAg                                                                                                                    >  2:710723/1‑139 (MQ=255)                         ttATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTaa                                                                                                           <  1:710723/139‑1 (MQ=255)                          tataTTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAAc                                                                                                          <  1:621951/139‑1 (MQ=255)                            tatTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAAccc                                                                                                        <  1:220330/139‑1 (MQ=255)                             atTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCtgtg                                                                                                                       >  1:88132/1‑123 (MQ=255)                             atTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCg                                                                                                       >  2:568640/1‑139 (MQ=255)                             atTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCAAGC‑TCTTCtgtg                                                                                                                       <  2:88132/123‑1 (MQ=255)                                 ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGgtgt                                                                                                   <  2:59231/139‑1 (MQ=255)                                 ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGgtgt                                                                                                   >  2:266160/1‑139 (MQ=255)                                      tatGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTg                                                                                              <  1:113987/139‑1 (MQ=255)                                             tttGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATg                                                                                                                                  <  2:604900/97‑1 (MQ=255)                                             tttGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATg                                                                                                                                  >  1:604900/1‑97 (MQ=255)                                                         cAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCAttttt                                                                           >  1:685416/1‑139 (MQ=255)                                                              gTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTa                                                                      <  1:266477/139‑1 (MQ=255)                                                               ttCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATg                                                                                                                                  >  1:393659/1‑79 (MQ=255)                                                               ttCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATg                                                                                                                                  <  2:393659/79‑1 (MQ=255)                                                                tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTAcc                                                                    >  2:347567/1‑139 (MQ=255)                                                                 cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAAcc                                                                                                         <  1:324517/101‑1 (MQ=255)                                                                 cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAAcc                                                                                                         >  2:324517/1‑101 (MQ=255)                                                                 cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGgtgt                                                                                                   <  2:29144/107‑1 (MQ=255)                                                                 cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGgtgt                                                                                                   >  1:29144/1‑107 (MQ=255)                                                                 cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCa                                                                   >  2:464592/1‑139 (MQ=255)                                                                           aTTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCattat                                                                                   >  1:597027/1‑113 (MQ=255)                                                                           aTTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCattat                                                                                   <  2:597027/113‑1 (MQ=255)                                                                           aTTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAagagag                                                         >  1:68252/1‑139 (MQ=255)                                                                                     aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCtgt                                                                                                                        <  2:322132/66‑1 (MQ=255)                                                                                     aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCtgt                                                                                                                        >  1:322132/1‑66 (MQ=255)                                                                                          ggATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAAccc                                                                                                        <  1:589050/77‑1 (MQ=255)                                                                                          ggATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAAccc                                                                                                        >  2:589050/1‑77 (MQ=255)                                                                                           gATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgc                                         >  2:612132/1‑139 (MQ=255)                                                                                            aTCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcg                                        >  2:243318/1‑139 (MQ=255)                                                                                               gCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcgcgc                                     <  1:568640/139‑1 (MQ=255)                                                                                                 cGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGa                                   >  2:638772/1‑139 (MQ=255)                                                                                                     cAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAaga                                                            <  2:178839/110‑1 (MQ=255)                                                                                                     cAGCGGGCGGCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAaga                                                            >  1:178839/1‑110 (MQ=255)                                                                                                        cgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAAccc                                                                                                        <  2:262983/63‑1 (MQ=255)                                                                                                        cgggcggCTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAAccc                                                                                                        >  1:262983/1‑63 (MQ=255)                                                                                                          ggcggcTGTCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCActc                          >  1:638234/1‑139 (MQ=255)                                                                                                                  tCATTTTATAAAATAAAGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCaa                  >  1:184779/1‑139 (MQ=255)                                                                                                                          taaaataaaGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcgtcgt          <  1:243318/139‑1 (MQ=255)                                                                                                                               taaaGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGta     <  2:638234/139‑1 (MQ=255)                                                                                                                                 aaGTAATCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGtaat   <  2:68252/139‑1 (MQ=255)                                                                                                                                  agtaatCTCATGC‑TCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGtaata  >  1:679295/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                        AACAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATA  >  NC_000913/2860283‑2860551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑