Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	468,510	A→G	E43G (GAG→GGG)	ybaO →	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	468,510	0	A	G	100.0%	92.6 / NA	27	E43G (GAG→GGG)	ybaO	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/9);  total (18/9)

GGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTC  >  NC_000913/468379‑468634                                                                                                                                     |                                                                                                                             ggCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgacgac                                                                                                                       <  1:683192/139‑1 (MQ=255)   cTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACgg                                                                                                                     >  2:433462/1‑139 (MQ=255)            aTAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTAt                                                                                                            >  1:88017/1‑139 (MQ=255)                aaTTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGc                                                                                                        >  1:512162/1‑139 (MQ=255)                aaTTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGc                                                                                                        >  1:271580/1‑139 (MQ=255)                               gctgGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCctgctg                                                                                         >  1:311264/1‑139 (MQ=255)                                        aCTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAGCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGaa                                                                                >  1:494897/1‑139 (MQ=255)                                                  gATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGcc                                                                      >  2:534336/1‑139 (MQ=255)                                                         cccTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCt                                                               <  2:418815/139‑1 (MQ=255)                                                                   gCAGGCACTGGCTGAAGCCGTTAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCtttt                                                     >  1:384515/1‑139 (MQ=255)                                                                        cACTGGCTGAAGCCGTTAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGGTCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCt                                                >  2:257387/1‑139 (MQ=255)                                                                        cACTGGCTGAAGCCGTTAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCt                                                >  1:204519/1‑139 (MQ=255)                                                                          cTGGCTGAAGCCGTTAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCg                                                          <  1:112117/127‑1 (MQ=255)                                                                          cTGGCTGAAGCCGTTAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCg                                                          >  2:112117/1‑127 (MQ=255)                                                                            ggCTGAAGCCGTTAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCtttt                                                     >  1:563695/1‑130 (MQ=255)                                                                            ggCTGAAGCCGTTAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCtttt                                                     <  2:563695/130‑1 (MQ=255)                                                                            ggCTGAAGCCGTTAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATa                                            >  1:705436/1‑139 (MQ=255)                                                                              cTGAAGCCGTTAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATaaa                                          >  1:562713/1‑139 (MQ=255)                                                                                       ttAATCTGACAACCACCCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAAc                                  >  1:411410/1‑139 (MQ=255)                                                                                        tAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACAt                                >  1:439025/1‑139 (MQ=255)                                                                                        tAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACAt                                <  1:257387/139‑1 (MQ=255)                                                                                            cTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATcaca                            <  1:433462/139‑1 (MQ=255)                                                                                               aCAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCAcagca                         <  2:384515/139‑1 (MQ=255)                                                                                                 aaCCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACagcagc                       <  2:88017/139‑1 (MQ=255)                                                                                                       ccccTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAAt                                                                           <  2:171715/82‑1 (MQ=255)                                                                                                       ccccTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAAt                                                                           >  1:171715/1‑82 (MQ=255)                                                                                                                       ccTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTc  >  1:192052/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                             GGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCA‑CCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTC  >  NC_000913/468379‑468634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑