Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,875,056	(C)6→5	coding (521/822 nt)	yeaM ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,875,051	0	C	.	100.0%	218.9 / NA	54	coding (526/822 nt)	yeaM	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (25/29);  total (25/29)

TTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTG  >  NC_000913/1874914‑1875181                                                                                                                                          |                                                                                                                                   tttCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcc                                                                                                                                   <  1:1258771/139‑1 (MQ=255)     aCTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccca                                                                                                                               <  2:955568/139‑2 (MQ=255)           cccTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGAc                                                                                                                        <  1:1494668/139‑1 (MQ=255)            ccTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACa                                                                                                                       >  2:728300/1‑139 (MQ=255)              tGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGc                                                                                                                                    >  2:1613061/1‑125 (MQ=255)              tGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGc                                                                                                                                    <  1:1613061/125‑1 (MQ=255)              tGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAgg                                                                                                                     >  2:1132292/1‑139 (MQ=255)                   cgcCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCtt                                                                                                                >  2:187953/1‑139 (MQ=255)                    gcCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCttt                                                                                                               <  1:544368/139‑1 (MQ=255)                     ccATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑TCCCCACTCGACAGGCCCTTTc                                                                                                              >  1:1212874/1‑139 (MQ=255)                           tCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                        <  1:969424/139‑1 (MQ=255)                           tCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                        <  1:728300/139‑1 (MQ=255)                              gCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCatcatc                                                                                                     >  1:1381287/1‑139 (MQ=255)                                       gctgACGCGACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATc                                                                                            >  1:1127820/1‑139 (MQ=255)                                         tgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTTACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGt                                                                                          >  1:1729339/1‑139 (MQ=255)                                         tgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                        <  1:557101/125‑1 (MQ=255)                                         tgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                        >  2:557101/1‑125 (MQ=255)                                           aCGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTTACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGc                                                                                        <  2:1729339/139‑1 (MQ=255)                                                     cGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAgg                                                                              <  1:1705434/139‑1 (MQ=255)                                                          aCTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATgag                                                                         <  1:1176208/139‑1 (MQ=255)                                                             cAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGagaag                                                                      <  2:473907/139‑1 (MQ=255)                                                                     tctcTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcccccact                                                                                                                             <  2:1481350/77‑4 (MQ=255)                                                                     tctcTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcccccact                                                                                                                             >  1:1481350/1‑74 (MQ=255)                                                                      ctctTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCca                                                                                                          <  2:1231999/94‑1 (MQ=255)                                                                      ctctTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCca                                                                                                          >  1:1231999/1‑94 (MQ=255)                                                                          tttACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTg                                                         >  1:1353737/1‑139 (MQ=255)                                                                          tttACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTg                                                         <  2:1381287/139‑1 (MQ=255)                                                                              cTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGTCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGc                                                     <  2:1127820/139‑1 (MQ=255)                                                                              cTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGc                                                     >  1:458963/1‑139 (MQ=255)                                                                               tATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCt                                                    <  2:249590/139‑1 (MQ=255)                                                                                   aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttg                                                >  1:1461245/1‑139 (MQ=255)                                                                                   aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttg                                                >  1:972868/1‑139 (MQ=255)                                                                                     tCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttgtt                                              >  1:1420714/1‑139 (MQ=255)                                                                                        ggCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCca                                                                                                          <  2:1530343/76‑1 (MQ=255)                                                                                        ggCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCca                                                                                                          >  1:1530343/1‑76 (MQ=255)                                                                                        ggCAAGATTGCCTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTtgtg                                           <  1:1132292/139‑1 (MQ=255)                                                                                         gCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGcc                                                                                                           <  1:1263452/74‑1 (MQ=255)                                                                                         gCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGcc                                                                                                           >  2:1263452/1‑74 (MQ=255)                                                                                         gCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTgg                                          <  2:1353737/139‑1 (MQ=255)                                                                                                  cGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAgg                                 <  1:187953/139‑1 (MQ=255)                                                                                                      cACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGt                             <  2:1420714/139‑1 (MQ=255)                                                                                                       aCTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑TCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa                               <  1:729797/136‑1 (MQ=255)                                                                                                       aCTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑TCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa                               >  2:729797/1‑136 (MQ=255)                                                                                                        cTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTtc                           >  2:1088282/1‑139 (MQ=255)                                                                                                           aTCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTtcatc                        >  2:367943/1‑139 (MQ=255)                                                                                                            tCGCAAAAAAGCCAGCCCATTGCCCCAATGcccccact                                                                                                                             <  1:1337533/38‑4 (MQ=255)                                                                                                            tCGCAAAAAAGCCAGCCCATTGCCCCAATGcccccact                                                                                                                             >  2:1337533/1‑35 (MQ=255)                                                                                                             cGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTg                                                                                         <  2:800956/72‑1 (MQ=255)                                                                                                             cGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTg                                                                                         >  1:800956/1‑72 (MQ=255)                                                                                                                 aaaaaGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATga                                                                          >  2:1517961/1‑83 (MQ=255)                                                                                                                 aaaaaGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATga                                                                          <  1:1517961/83‑1 (MQ=255)                                                                                                                    aaGCCAGCCCATTGCCCCAATG‑TCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGAc               <  2:1212874/139‑1 (MQ=255)                                                                                                                     agccagccCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGcc                                                                                                           >  2:119761/1‑46 (MQ=39)                                                                                                                     agccagccCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGcc                                                                                                           <  1:119761/46‑1 (MQ=39)                                                                                                                          gccCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCt                                                    >  2:618517/1‑96 (MQ=255)                                                                                                                          gccCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCt                                                    <  1:618517/96‑1 (MQ=255)                                                                                                                            ccATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTa       <  2:458963/139‑1 (MQ=255)                                                                                                                                tGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa                               >  1:1543995/1‑111 (MQ=255)                                                                                                                                tGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa                               <  2:1543995/111‑1 (MQ=255)                                                                                                                                tGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCt   >  1:324442/1‑139 (MQ=255)                                                                                                                                 gCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTg  <  2:1461245/139‑1 (MQ=255)                                                                                                                                 gCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATAATCTCCACCTTCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTg  <  1:1088282/139‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   TTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTG  >  NC_000913/1874914‑1875181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑