| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,462,889 | (C)9→8 | intergenic (+243/‑123) | fadL → / → yfdF | long‑chain fatty acid outer membrane transporter/uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,462,881 | 0 | C | . | 97.8% | 179.2 / ‑2.8 | 46 | intergenic (+235/‑131) | fadL/yfdF | long‑chain fatty acid outer membrane transporter/uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (20/25); total (20/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
AACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGG > NC_000913/2462748‑2462969 | aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTcccccc < 2:331088/139‑1 (MQ=255) aCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTccccccc < 2:749871/139‑1 (MQ=255) ggCAGCTTTCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTcc > 1:1124925/1‑124 (MQ=255) ggCAGCTTGCCCTTTCAAATCAATAAGCAGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTcc < 2:1124925/124‑1 (MQ=255) aGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtgtg < 1:499081/138‑1 (MQ=255) aGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtgtg > 2:499081/1‑138 (MQ=255) gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTgcg < 2:185791/139‑1 (MQ=255) gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTgcg > 1:1575453/1‑139 (MQ=255) gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTgcg < 2:281897/139‑1 (MQ=255) cTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTgcgc > 1:1386264/1‑139 (MQ=255) ccTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATa < 2:598509/139‑1 (MQ=255) ttCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATacac < 1:132407/139‑1 (MQ=255) ttCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATacac > 2:132407/1‑139 (MQ=255) aTCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTcccccccctgg < 1:668781/115‑4 (MQ=255) aTCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTcccccccctgg > 2:668781/1‑112 (MQ=255) tAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTaaa > 1:1362578/1‑139 (MQ=255) aGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTGTCAGGCGTTCTGCTCGTCCTTCTCAATGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑‑CCCCCCCTGGCTATTGTGCGCTCATACGCTCAAATTAAAGAt < 1:1052179/139‑1 (MQ=255) aGCGGTATCAAAAACCAAACCTGAAGGAAAAGCCATTTTTCCATG‑GTTCTTGTCGGCCCTCCTAAAGGGTTTACTTTTCCTCCATTCGCAGAATA‑T‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt < 1:1061459/139‑1 (MQ=255) gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt < 2:1362578/139‑1 (MQ=255) cGGTATCCACAGACAAACCTGAAGGGAAAGTCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt < 1:201433/139‑1 (MQ=255) aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt < 2:1575453/139‑1 (MQ=255) tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTcccccc < 2:707062/96‑1 (MQ=255) tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTcccccc > 1:707062/1‑96 (MQ=255) aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTcccccccc < 2:716971/93‑1 (MQ=255) aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTcccccccc > 1:716971/1‑93 (MQ=255) aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGCTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTcccccccc > 2:1298063/1‑93 (MQ=255) aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGCTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTcccccccc < 1:1298063/93‑1 (MQ=255) cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTgcg > 2:1177213/1‑103 (MQ=255) cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTgcg < 1:1177213/103‑1 (MQ=255) cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt > 2:26242/1‑125 (MQ=255) cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt < 1:26242/125‑1 (MQ=255) aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTcccccc < 2:271958/86‑1 (MQ=255) aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTcccccc > 1:271958/1‑86 (MQ=255) aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATg > 1:672818/1‑139 (MQ=255) aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATg > 1:1047182/1‑139 (MQ=255) ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg > 2:48757/1‑139 (MQ=255) ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg > 1:723677/1‑139 (MQ=255) aaGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTcc > 1:681880/1‑76 (MQ=255) aaGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTcc < 2:681880/76‑1 (MQ=255) aaaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTg > 2:564542/1‑139 (MQ=255) aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa < 1:777292/139‑1 (MQ=255) aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa < 1:564542/139‑1 (MQ=255) aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa < 2:1047182/139‑1 (MQ=255) ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt < 2:672818/139‑1 (MQ=255) gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtgtg < 2:1626502/70‑1 (MQ=255) gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtgtg > 1:1626502/1‑70 (MQ=255) gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg < 2:1386264/139‑1 (MQ=255) gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg < 1:48757/139‑1 (MQ=255) ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTcccc < 1:1572636/54‑1 (MQ=255) ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTcccc > 2:1572636/1‑54 (MQ=255) ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt < 2:1044167/93‑1 (MQ=255) ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt > 1:1044167/1‑93 (MQ=255) cTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtgtg > 2:1590102/1‑67 (MQ=255) cTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtgtg < 1:1590102/67‑1 (MQ=255) cGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTcccccccc > 2:632311/1‑51 (MQ=255) cGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTcccccccc < 1:632311/51‑1 (MQ=255) tCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa < 2:1668336/114‑1 (MQ=255) tCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa > 1:1668336/1‑114 (MQ=255) tctcAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTgcg < 2:248345/58‑1 (MQ=255) tctcAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTgcg > 1:248345/1‑58 (MQ=255) tttACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCa > 2:208851/1‑52 (MQ=255) tttACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCa < 1:208851/52‑1 (MQ=255) tACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt > 1:797882/1‑68 (MQ=255) tACTTTT‑CTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt < 2:797882/68‑1 (MQ=255) ccAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATacac > 2:1482929/1‑40 (MQ=255) ccAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATacac < 1:1482929/40‑1 (MQ=255) | AACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTT‑CTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGG > NC_000913/2462748‑2462969 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |