Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,203,986	(A)5→6	coding (15/318 nt)	yehK →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,203,981	1	.	A	98.6%	234.5 / ‑3.9	72	coding (10/318 nt)	yehK	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base A (32/39);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

GATGTGTTTGATTTGAGTGAGGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGC‑AAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGAATTATTTACAGGTTCCGTCTATCGACTGGAG  >  NC_000913/2203858‑2204100                                                                                                                              |                                                                                                                         gATGTGTTTGATTTGAGTGAGGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGtt                                                                                                           >  2:1114891/1‑139 (MQ=255)   tgtgTTTGATTTGAGTGAGGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGt                                                                                                            >  2:145857/1‑136 (MQ=255)   tgtgTTTGATTTGAGTGAGGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGt                                                                                                            <  1:145857/136‑1 (MQ=255)       tttgatttgaGTGAGGCGTTAAGCGCACCTGACGTCATTTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTAt                                                                                                      >  1:1322381/1‑139 (MQ=255)       tttgatttgaGTGAGGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTAtt                                                                                                     >  2:821384/1‑139 (MQ=255)       tttgatttgaGTGAGGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGC‑aaaaaagag                                                                                                                 >  1:618366/1‑123 (MQ=255)       tttgatttgaGTGAGGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGC‑aaaaaagag                                                                                                                 >  1:1681694/1‑123 (MQ=255)       tttgatttgaGTGAGGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGC‑aaaaaagag                                                                                                                 <  2:618366/127‑5 (MQ=255)       tttgatttgaGTGAGGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGC‑aaaaaagag                                                                                                                 <  2:1681694/127‑5 (MQ=255)         tgatttgaGTGAGGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTa                                                                                                   >  1:247931/1‑139 (MQ=255)         tgatttgaGTGAGGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTa                                                                                                   <  2:744522/139‑1 (MQ=255)           atttgaGTGAGGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACg                                                                                                 <  2:473605/139‑1 (MQ=255)           atttgaGTGAGGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACg                                                                                                 <  1:592359/139‑1 (MQ=255)           atttgaGTGAGGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGC‑aaaaaaga                                                                                                                  <  2:55490/122‑4 (MQ=255)           atttgaGTGAGGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGC‑aaaaaaga                                                                                                                  >  1:55490/1‑119 (MQ=255)             ttgaGTGAGGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGAt                                                                                               >  1:961216/1‑139 (MQ=255)               gagtgagGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTa                                                                                             >  2:1172094/1‑139 (MQ=255)                agtgagGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTAt                                                                                            >  2:1091221/1‑139 (MQ=255)                 gtgagGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATg                                                                                           <  1:1091221/139‑1 (MQ=255)                   gagGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAg                                                                                         <  1:121324/139‑1 (MQ=255)                   gagGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAg                                                                                         <  1:1172094/139‑1 (MQ=255)                    agGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAgg                                                                                        >  2:1056529/1‑139 (MQ=255)                     ggCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGt                                                                                       <  2:961216/139‑1 (MQ=255)                        gTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTccc                                                                                    <  2:1390806/139‑1 (MQ=255)                            aGCGCACCTGACGTCATTTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGt                                                                                 <  2:1322381/139‑1 (MQ=255)                                cACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTAtt                                                                                                     >  1:1615784/1‑114 (MQ=255)                                cACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTAtt                                                                                                     <  2:1615784/114‑1 (MQ=255)                                   cTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAg                                                                         >  1:1475319/1‑139 (MQ=255)                                      aCGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATc                                                                      >  2:1214464/1‑139 (MQ=255)                                          cA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGtt                                                                  <  2:1663387/139‑1 (MQ=255)                                           a‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGttt                                                                 >  2:933941/1‑139 (MQ=255)                                              tttCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTc                                                               >  1:786563/1‑139 (MQ=255)                                                 ccATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGTTTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCtt                                                            <  1:1317497/139‑1 (MQ=255)                                                   aTTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCa                                                          <  2:247931/139‑1 (MQ=255)                                                         acacAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTg                                                    <  1:5817/139‑1 (MQ=255)                                                          cacaGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTga                                                   <  2:1475319/139‑1 (MQ=255)                                                           acaGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTgag                                                  <  2:495218/139‑1 (MQ=255)                                                            caGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTgaga                                                 <  1:939406/139‑1 (MQ=255)                                                             aGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTg                                                                                                          >  1:1683663/1‑81 (MQ=255)                                                             aGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTg                                                                                                          <  2:1683663/81‑1 (MQ=255)                                                               cGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTc                                                                                      <  1:1355217/99‑1 (MQ=255)                                                               cGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTc                                                                                      >  2:1355217/1‑99 (MQ=255)                                                               cGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTga                                                   <  2:735875/134‑1 (MQ=255)                                                               cGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTga                                                   >  1:735875/1‑134 (MQ=255)                                                                 ggCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCa                                                          >  1:1507401/1‑125 (MQ=255)                                                                 ggCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCa                                                          <  2:1507401/125‑1 (MQ=255)                                                                 ggCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCAt                                            >  1:18016/1‑139 (MQ=255)                                                                   cAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATg                                                                                           <  2:666610/90‑1 (MQ=255)                                                                   cAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATg                                                                                           >  1:666610/1‑90 (MQ=255)                                                                   cAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATaa                                          <  1:1114891/139‑1 (MQ=255)                                                                         tGCAACTACCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGC‑aaaaaagag                                                                                                                 >  1:1537907/1‑58 (MQ=255)                                                                         tGCAACTACCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGC‑aaaaaagag                                                                                                                 <  2:1537907/62‑5 (MQ=255)                                                                            aaCTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGa                                 >  1:1425347/1‑139 (MQ=255)                                                                            aaCTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGa                                 <  2:1425347/139‑1 (MQ=255)                                                                             aCTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGaa                                <  1:821384/139‑1 (MQ=255)                                                                                gCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTg                                                                                                              >  1:1513703/1‑58 (MQ=255)                                                                                gCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTg                                                                                                              <  2:1513703/58‑1 (MQ=255)                                                                                gCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTAttt                                                                                                    <  2:191784/68‑1 (MQ=255)                                                                                gCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTAttt                                                                                                    >  1:191784/1‑68 (MQ=255)                                                                                gCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTa                                                                                                   <  1:511548/69‑1 (MQ=255)                                                                                gCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTa                                                                                                   >  2:511548/1‑69 (MQ=255)                                                                                gCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTccc                                                                                    <  2:1648316/84‑1 (MQ=255)                                                                                gCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTccc                                                                                    >  1:1648316/1‑84 (MQ=255)                                                                                 cccGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGaa                                >  2:1547469/1‑135 (MQ=255)                                                                                 cccGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGaa                                <  1:1547469/135‑1 (MQ=255)                                                                                  ccGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGAattatt                           >  2:1188080/1‑139 (MQ=255)                                                                                       aTCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGAATTATTTACAg                      <  2:18016/139‑1 (MQ=255)                                                                                           acacGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAA‑AGCTGGTTg                                                                                                          <  2:484456/51‑1 (MQ=255)                                                                                           acacGACTTACCAGCGGGGAAAGCGATGATCGTGC‑AAAAAAAAGCTGGTTg                                                                                                          >  1:484456/1‑51 (MQ=255)                                                                                             acGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTg                                                                                  <  2:813533/73‑1 (MQ=255)                                                                                             acGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTg                                                                                  >  1:813533/1‑73 (MQ=255)                                                                                              cGACTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGAATTATTTACAGGTTCCGt               <  1:995630/139‑1 (MQ=255)                                                                                                 cTTACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTTAGATCCATAAATGCTCTGAATTATTTACAGGTTCCGTCTa            <  1:1056529/139‑1 (MQ=255)                                                                                                  ttACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTccc                                                                                    >  2:492207/1‑66 (MQ=255)                                                                                                  ttACCAGCGGGGAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTccc                                                                                    <  1:492207/66‑1 (MQ=255)                                                                                                           gggAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGAc                                                       <  2:570378/86‑1 (MQ=255)                                                                                                           gggAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGAc                                                       >  1:570378/1‑86 (MQ=255)                                                                                                           gggAAAGCGATGATCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGAATTATTTACAGGTTCCGTCTATCGACTGGAg  >  2:1059400/1‑139 (MQ=255)                                                                                                                      gatCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTg                                                    <  1:1300270/78‑1 (MQ=255)                                                                                                                      gatCGTGCAAAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTg                                                    >  2:1300270/1‑78 (MQ=255)                                                                                                                              |                                                                                                                         GATGTGTTTGATTTGAGTGAGGCGTTAAGCGCACCTGACGTCA‑TTTTCCATTAAAACACAGCGGGCAGTGATGCAACTGCCCGTTATCAACACGACTTACCAGCGGGGAAAGCGATGATCGTGC‑AAAAAG‑AGCTGGTTGCTATTTACGATTATGAGGTCCCTGTACCTGAAGATCCGTTTTCCTTCAGACTTGAGATCCATAAATGCTCTGAATTATTTACAGGTTCCGTCTATCGACTGGAG  >  NC_000913/2203858‑2204100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑