| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,474,138 | (A)6→7 | coding (15/594 nt) | bdcR → | transcriptional repressor for divergent bdcA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,474,132 | 1 | . | A | 98.5% | 214.2 / ‑3.9 | 67 | coding (9/594 nt) | bdcR | transcriptional repressor for divergent bdcA |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (34/32); total (35/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.84e-01 | |||||||||||
ACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGT > NC_000913/4474006‑4474264 | aCGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAGAAAACAAtc > 1:1108538/1‑139 (MQ=255) gACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAAtctc < 2:1435001/139‑1 (MQ=255) gACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAAtctc < 1:1170744/139‑1 (MQ=255) ccACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGtt > 2:1598957/1‑139 (MQ=255) ccACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGtt > 1:713387/1‑139 (MQ=255) cGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAg < 1:1338579/139‑1 (MQ=255) aGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGgt < 2:1108538/139‑1 (MQ=255) cTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGttt < 2:713387/139‑1 (MQ=255) aCCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGc > 1:603050/1‑139 (MQ=255) tAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTg < 1:1598957/139‑1 (MQ=255) aaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg < 1:237725/139‑1 (MQ=255) cATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg > 2:974361/1‑131 (MQ=255) cATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg < 1:974361/131‑1 (MQ=255) tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATTGTCACTAAA‑AAAACAATCTcg < 2:1041125/88‑1 (MQ=255) tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTcg > 1:1041125/1‑88 (MQ=255) tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGc > 2:1468367/1‑139 (MQ=255) tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGc > 2:624663/1‑139 (MQ=255) cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAgg > 1:757313/1‑95 (MQ=255) cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAgg < 2:757313/95‑1 (MQ=255) cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTg < 2:1668712/118‑1 (MQ=255) cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTg > 1:1668712/1‑118 (MQ=255) ttattaTGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCg > 1:65006/1‑129 (MQ=255) ttattaTGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCg < 2:65006/129‑1 (MQ=255) attatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCtt > 1:1345456/1‑139 (MQ=255) atGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCttttt > 2:326055/1‑139 (MQ=255) aTGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaa < 2:171073/139‑1 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGgt < 2:785940/77‑1 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGgt > 1:785940/1‑77 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAGAGTGCTTTTTCACCaaaa > 1:1167482/1‑139 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCttt > 2:1562865/1‑129 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCttt < 1:1562865/129‑1 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa > 1:840910/1‑139 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa > 1:1427860/1‑139 (MQ=255) aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTcg < 1:943048/64‑1 (MQ=255) aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTcg > 2:943048/1‑64 (MQ=255) tACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGtt < 2:1345456/139‑1 (MQ=255) cAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGc > 2:1712759/1‑108 (MQ=255) cAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGc < 1:1712759/108‑1 (MQ=255) aaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg > 2:54704/1‑91 (MQ=255) aaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg < 1:54704/91‑1 (MQ=255) cTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTc < 1:1276957/84‑1 (MQ=255) cTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAAGCCCGCGTTCCAGGTCGTCCCAGACGTTTCGCTc > 2:1276957/1‑84 (MQ=255) ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt > 1:1636107/1‑139 (MQ=255) ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt < 2:1427860/139‑1 (MQ=255) aCTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGgt < 1:173657/57‑1 (MQ=255) aCTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGgt > 2:173657/1‑57 (MQ=255) aCTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAgtg > 2:309948/1‑139 (MQ=255) cTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaa < 2:147303/113‑1 (MQ=255) cTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaa > 1:147303/1‑113 (MQ=255) cTGTCAACAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCt > 1:129544/1‑139 (MQ=255) aaCAGGTTTTGTGATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGa < 1:759154/56‑1 (MQ=255) aaCAGGTTTTGTGATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGa > 2:759154/1‑56 (MQ=255) aaCAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCa > 1:1039639/1‑77 (MQ=255) aaCAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCa < 2:1039639/77‑1 (MQ=255) cAGGTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTa < 2:129544/139‑1 (MQ=255) gTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGc < 1:1020848/128‑1 (MQ=255) gTTTTGTAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTCTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGc > 2:1020848/1‑128 (MQ=255) tAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGa < 2:563824/134‑1 (MQ=255) tAATGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGa > 1:563824/1‑134 (MQ=255) aaTGGTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCt < 2:603050/139‑1 (MQ=255) ggTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGttt < 2:226609/46‑1 (MQ=255) ggTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGttt > 1:226609/1‑46 (MQ=255) ggTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAg > 2:1690782/1‑112 (MQ=255) ggTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAg < 1:1690782/112‑1 (MQ=255) ggTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGa < 2:170476/130‑1 (MQ=255) ggTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGa > 1:170476/1‑130 (MQ=255) gTCACTAAA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGt > 2:824595/1‑139 (MQ=255) | ACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AA‑AAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGT > NC_000913/4474006‑4474264 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |