| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,609,336 | (C)7→8 | intergenic (+13/‑78) | yjjG → / → prfC | dUMP phosphatase/peptide chain release factor RF‑3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,609,329 | 1 | . | C | 90.9% | 102.8 / 2.8 | 33 | intergenic (+6/‑85) | yjjG/prfC | dUMP phosphatase/peptide chain release factor RF‑3 |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base C (17/13); total (18/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑T‑‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACTT > NC_000913/4609192‑4609459 | ctcGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑T‑‑c < 1:719859/139‑1 (MQ=255) tCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑T‑‑cccccc‑cccgt > 2:595810/1‑135 (MQ=255) cAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑T‑‑cccccc‑ccgttg < 1:462974/139‑6 (MQ=255) cAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑T‑‑cccccc‑ccgttg < 2:1417018/139‑6 (MQ=255) cGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGt > 1:130093/1‑139 (MQ=255) cGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑T‑‑cccccc‑ccgttg < 2:1227842/133‑6 (MQ=255) cGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑T‑‑cccccc‑ccgttg > 1:1227842/1‑128 (MQ=255) ttGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAAt < 1:1588293/139‑1 (MQ=255) tGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑TCCCCCCCC‑CGTTGATGGGTAAAAt < 1:595810/139‑1 (MQ=255) cGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGc > 1:203110/1‑139 (MQ=255) cATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAg < 2:130093/139‑1 (MQ=255) aTCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTTGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑T‑‑cccccc‑ccgttg > 2:356418/1‑96 (MQ=255) aTCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑T‑‑cccccc‑ccgttg < 1:356418/101‑6 (MQ=255) tCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑T‑‑cccc > 1:68517/1‑92 (MQ=255) tCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑T‑‑cccc < 2:68517/92‑1 (MQ=255) tCGCGAGCAACCAGAAGGCATCGCGACCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCCCAATTTTTCGTTTTCAACAAgcg > 1:1414416/1‑139 (MQ=255) aaCCAGAAGGCATCGCGCCCACCTGGAGCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTcaac > 1:1121917/1‑126 (MQ=255) aaCCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTT‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTcaac < 2:1121917/126‑1 (MQ=255) actgTTTCTTCGTTGCGAGAGCTGGGGCTGCTCCGGTGTAAATACGGTTGG‑CCAC‑‑CCCCCC‑CGTTGATGGGTAAAATAGCTGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCATACTCAAGAAGAAAGAAtt < 2:203110/136‑1 (MQ=255) ttttttCGTTGCACGAACTGGAGCAGCTCCTGTTTAAACACTGATTGCCC‑C‑‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAAttttt < 2:807468/86‑1 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCC‑C‑‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAAttttt > 1:807468/1‑86 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCa < 2:190811/80‑1 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCa > 1:190811/1‑80 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTTCTCAAGAAGAAAGAATTATGa > 1:400783/1‑139 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaa < 1:463450/123‑1 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaa > 2:463450/1‑123 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGa > 2:892620/1‑139 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGa > 1:965259/1‑139 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑T‑‑ccc < 1:281049/53‑1 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑T‑‑ccc > 2:281049/1‑53 (MQ=255) tcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑T‑‑cccccc‑ccgttg < 2:1559331/60‑6 (MQ=255) tcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑T‑‑cccccc‑ccgttg > 1:1559331/1‑55 (MQ=255) ttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGtt < 2:1414416/139‑1 (MQ=255) cACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGAt < 1:259123/99‑1 (MQ=255) cACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGAt > 2:259123/1‑99 (MQ=255) cACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCt > 2:615788/1‑139 (MQ=255) agcagcTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAag < 1:892620/139‑1 (MQ=255) cTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGAt > 1:137843/1‑84 (MQ=255) cTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaa > 2:1646376/1‑97 (MQ=255) cTCCTGTGTAAACACTGATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaa < 1:1646376/97‑1 (MQ=255) cTCCTGTGTAAACACTGATTG‑CC‑T‑‑CCCCCCTCGTTGATGGGTAAAATAGCCGCAACTTTTCGTTTTCAACAAGCGCGGCGCGAt < 2:137843/84‑1 (MQ=255) gATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTAcg > 2:1097978/1‑77 (MQ=255) gATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACt < 1:1097978/78‑1 (MQ=255) gATTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACtt > 1:547632/1‑139 (MQ=255) aTTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAGTTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTc > 1:1158656/1‑78 (MQ=255) aTTG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCCCGATGCCGCTTACTc < 2:1158656/78‑1 (MQ=255) ttG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGcc > 1:1599434/1‑69 (MQ=255) ttG‑CC‑TC‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGcc < 2:1599434/69‑1 (MQ=255) | CTCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTG‑CC‑T‑‑CCCCCC‑CGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACTT > NC_000913/4609192‑4609459 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |