Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,260,528	A→G	I92I (ATT→ATC)	dauA ←	C4‑dicarboxylic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,260,528	0	A	G	100.0%	235.5 / NA	64	I92I (ATT→ATC)	dauA	C4‑dicarboxylic acid transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/34);  total (30/34)

GAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGAATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACA  >  NC_000913/1260410‑1260639                                                                                                                        |                                                                                                                 gAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGt                                                                                             <  2:1104809/139‑1 (MQ=255)                ggCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCt                                                                              >  2:1439229/1‑139 (MQ=255)                ggCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCt                                                                              >  2:1471910/1‑139 (MQ=255)                ggCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCt                                                                              >  2:1524334/1‑139 (MQ=255)                  cGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTaa                                                                            >  2:11914/1‑139 (MQ=255)                  cGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTaa                                                                            >  2:1205626/1‑139 (MQ=255)                   gACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACTGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTaaa                                                                           >  1:1031707/1‑139 (MQ=255)                    aCCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAg                                                                          <  1:1205626/139‑1 (MQ=255)                      cAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTaaa                                                                           <  1:356089/136‑1 (MQ=255)                      cAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTaaa                                                                           >  2:356089/1‑136 (MQ=255)                               ccAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCg                                                                                                 >  2:439068/1‑105 (MQ=255)                               ccAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCg                                                                                                 <  1:439068/105‑1 (MQ=255)                               ccAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGacccacc                                                               >  1:192354/1‑139 (MQ=255)                               ccAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGacc                                                                   >  2:283451/1‑135 (MQ=255)                               ccAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGacc                                                                   <  1:283451/135‑1 (MQ=255)                                   aCCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGaccca                                                                 >  2:1431100/1‑133 (MQ=255)                                   aCCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGaccca                                                                 <  1:1431100/133‑1 (MQ=255)                                       aTCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCagag                                                       <  1:974196/139‑1 (MQ=255)                                         cAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCa                                                     >  2:365751/1‑139 (MQ=255)                                            aaTCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATg                                                  >  2:173015/1‑139 (MQ=255)                                               cAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACa                                               <  2:1331726/139‑1 (MQ=255)                                                      aTCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATc                                            <  1:596520/135‑1 (MQ=255)                                                      aTCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATc                                            >  2:596520/1‑135 (MQ=255)                                                      aTCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCg                                        >  2:741207/1‑139 (MQ=255)                                                          ccGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGt                                                            >  2:974115/1‑115 (MQ=255)                                                          ccGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGt                                                            <  1:974115/115‑1 (MQ=255)                                                                gaaaGGTCCAAACCACAGGTCCTGCAAG‑CCAAACTTGTTGC‑ACAAGGGATAGAAGATTACCACAAAAGGCCGAGGTCGGCCCGGAACCGCTAAACCTTGACCACCCCGTAAGAGAATTGCCAA‑CCCCGCAACAGCTGCg                             <  1:1395698/137‑1 (MQ=255)                                                                gCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTgg                              <  1:173015/139‑2 (MQ=255)                                                                gCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGc                              <  1:1439229/139‑1 (MQ=255)                                                                gCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGc                              <  2:192354/139‑1 (MQ=255)                                                                gCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGc                              >  2:800199/1‑139 (MQ=255)                                                                 cagggTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGc                              <  1:279952/135‑1 (MQ=255)                                                                 cagggTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGGGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGc                              >  2:279952/4‑138 (MQ=255)                                                                 cAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCGGCAGTCGGACCGGAAACGCt                                                                              >  2:1011024/1‑90 (MQ=255)                                                                 cAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCt                                                                              <  1:1011024/90‑1 (MQ=255)                                                                 cAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCC‑CAACAGCTTCgg                            >  2:1395698/1‑139 (MQ=255)                                                                     gTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCATAGCACTGACAATCCCCGCAACAGCTGCGGtat                         <  2:1031707/139‑1 (MQ=255)                                                                         cAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATaa                     >  2:952047/1‑139 (MQ=255)                                                                          aaCCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTGCCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATaaa                    <  1:11914/139‑1 (MQ=255)                                                                                    ccTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTggg          <  1:1023769/139‑1 (MQ=255)                                                                                       gCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTg       <  1:1524334/139‑1 (MQ=255)                                                                                           gTCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCcac   <  1:1471910/139‑1 (MQ=255)                                                                                            tCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACa                                               >  1:345101/1‑94 (MQ=255)                                                                                            tCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACa                                               <  2:345101/94‑1 (MQ=255)                                                                                            tCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGc                              <  1:680358/111‑1 (MQ=255)                                                                                            tCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGc                              >  2:680358/1‑111 (MQ=255)                                                                                            tCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCcaca  <  1:671843/139‑1 (MQ=255)                                                                                            tCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCcaca  <  1:365751/139‑1 (MQ=255)                                                                                            tCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCcaca  <  1:1277206/139‑1 (MQ=255)                                                                                            tCCAAAC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCcaca  <  1:952047/139‑1 (MQ=255)                                                                                               aaaC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGt                                                            >  2:127805/1‑78 (MQ=255)                                                                                               aaaC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGt                                                            <  1:127805/78‑1 (MQ=255)                                                                                                aaC‑TGTTGCGACACGGGATAGAGGATTACGAC‑AAATGCCGCAGTCGGa                                                                                         >  1:1157655/1‑48 (MQ=39)                                                                                                aaC‑TGTTGCGACACGGGATAGAGGATTACCAC‑GAATGCCGCAGTCGGa                                                                                         <  2:1157655/48‑1 (MQ=255)                                                                                                 aC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCcaca  >  2:842371/1‑134 (MQ=255)                                                                                                 aC‑TGTTGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCcaca  <  1:842371/134‑1 (MQ=255)                                                                                                      ttGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAg                                  >  1:1707106/1‑98 (MQ=255)                                                                                                      ttGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAg                                  <  2:1707106/98‑1 (MQ=255)                                                                                                       tGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCa                                                          >  2:795640/1‑73 (MQ=255)                                                                                                       tGCGACACGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCa                                                          <  1:795640/73‑1 (MQ=255)                                                                                                           acacGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGc                              >  1:1729492/1‑97 (MQ=255)                                                                                                           acacGGGATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGc                              <  2:1729492/97‑1 (MQ=255)                                                                                                                ggATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGtat                         <  1:1439316/97‑1 (MQ=255)                                                                                                                ggATAGAGGATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGtat                         >  2:1439316/1‑97 (MQ=255)                                                                                                                        |                                                                                                                 GAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCAGTCCAAAC‑TGTTGCGACACGGGATAGAGAATTACCAC‑AAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACA  >  NC_000913/1260410‑1260639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑