Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,049,336	C→T	P1467S (CCG→TCG)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,049,336	0	C	T	100.0%	248.9 / NA	76	P1467S (CCG→TCG)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/42);  total (34/42)

GCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATCCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGATGTGAAGGCGAACTT  >  NC_000913/2049201‑2049457                                                                                                                                         |                                                                                                                          gCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCgg                                                                                                                        >  1:53145/1‑139 (MQ=255)      cTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGAtt                                                                                                                   <  2:476879/139‑1 (MQ=255)      cTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGAtt                                                                                                                   <  1:1346826/139‑1 (MQ=255)         tACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGcc                                                                                                                <  2:180504/139‑1 (MQ=255)            cGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGAc                                                                                                             <  1:1288157/139‑1 (MQ=255)               tACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAAt                                                                                                          >  2:46728/1‑139 (MQ=255)                 cAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACa                                                                                                            <  1:66855/135‑1 (MQ=255)                 cAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACa                                                                                                            >  2:66855/1‑135 (MQ=255)                  aaaaGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAAc                                                                                                       <  2:275509/139‑1 (MQ=255)                        cGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGcc                                                                                                                <  2:858648/124‑1 (MQ=255)                        cGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGcc                                                                                                                >  1:858648/1‑124 (MQ=255)                         ggggTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAGCAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCa                                                                                                >  2:1068733/1‑139 (MQ=255)                           ggTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACg                                                                                              >  2:465157/1‑139 (MQ=255)                            gTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGa                                                                                             <  2:907593/139‑1 (MQ=255)                              ttATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGaca                                                                                           <  2:807138/139‑1 (MQ=255)                              ttATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGaca                                                                                           <  1:1222745/139‑1 (MQ=255)                                aTCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGACGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGCTTCGGTGATTGCCGACAATAACGATCTCACGACACt                                                                                         >  2:1228456/1‑139 (MQ=255)                                     ttGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGAtctc                                                                                                 <  2:754008/126‑1 (MQ=255)                                     ttGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGAtctc                                                                                                 >  1:754008/1‑126 (MQ=255)                                     ttGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAg                                                                                    >  1:361404/1‑139 (MQ=255)                                       gACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGa                                                                                                              <  2:1070020/112‑1 (MQ=255)                                       gACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGa                                                                                                              >  1:1070020/1‑112 (MQ=255)                                          ggCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGa                                                                                                                     <  2:99967/101‑1 (MQ=255)                                          ggCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGa                                                                                                                     >  1:99967/1‑101 (MQ=255)                                          ggCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                                               <  1:1228456/139‑1 (MQ=255)                                          ggCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                                               <  2:42998/139‑1 (MQ=255)                                          ggCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                                               >  2:30067/1‑139 (MQ=255)                                          ggCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                                               <  2:1514815/139‑1 (MQ=255)                                          ggCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                                               >  2:1286209/1‑139 (MQ=255)                                            cAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGt                                                                             <  2:361404/139‑1 (MQ=255)                                              aCCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCg                                                                           <  1:465157/139‑1 (MQ=255)                                               cccTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGc                                                                          >  1:279083/1‑139 (MQ=255)                                               cccTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGc                                                                          <  1:1286209/139‑1 (MQ=255)                                               cccTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGc                                                                          >  2:228213/1‑139 (MQ=255)                                               cccTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGc                                                                          >  1:1691288/1‑139 (MQ=255)                                                          ggCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGa                                                                                                              <  1:1253987/92‑1 (MQ=255)                                                          ggCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAgg                                                               >  1:614118/1‑139 (MQ=255)                                                          ggCGATTCGATGCAACAAACAGGGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGa                                                                                                              >  2:1253987/1‑92 (MQ=255)                                                           gCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAggg                                                              <  1:30067/139‑1 (MQ=255)                                                             gATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCa                                                            <  2:305932/139‑1 (MQ=255)                                                              aTTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCaa                                                           >  1:1635625/1‑139 (MQ=255)                                                                tCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATg                                                         <  2:279083/139‑1 (MQ=255)                                                                tCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATg                                                         <  2:1691288/139‑1 (MQ=255)                                                                        acaaacaGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGcc                                                 <  2:144214/139‑1 (MQ=255)                                                                            acaGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAaca                                             <  1:1271334/139‑1 (MQ=255)                                                                                   ccTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGt                                      <  2:977040/139‑1 (MQ=255)                                                                                      aTGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCa                                                            <  1:165840/114‑1 (MQ=255)                                                                                      aTGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCa                                                            >  2:165840/1‑114 (MQ=255)                                                                                      aTGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCg                                                       >  2:1346093/1‑119 (MQ=255)                                                                                      aTGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCg                                                       <  1:1346093/119‑1 (MQ=255)                                                                                       tgtgCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCAc                                                                                               <  2:718860/78‑1 (MQ=255)                                                                                       tgtgCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCAc                                                                                               >  1:718860/1‑78 (MQ=255)                                                                                        gtgCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACAt                                 >  1:988833/1‑139 (MQ=255)                                                                                          gCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACAttt                               >  1:1535892/1‑139 (MQ=255)                                                                                              aaCGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGAtctc                                                                                                 >  2:257681/1‑69 (MQ=255)                                                                                              aaCGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGAtctc                                                                                                 <  1:257681/69‑1 (MQ=255)                                                                                              aaCGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTActc                           >  1:534765/1‑139 (MQ=255)                                                                                              aaCGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTAct                            >  1:687438/1‑138 (MQ=255)                                                                                              aaCGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTAct                            <  2:687438/138‑1 (MQ=255)                                                                                               aCGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAATGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTActct                          <  1:1068733/139‑1 (MQ=255)                                                                                               aCGTCGCAAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTActct                          <  1:228213/139‑1 (MQ=255)                                                                                                   cgcgAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGaca                                                                                           >  1:654841/1‑70 (MQ=255)                                                                                                   cgcgAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGaca                                                                                           <  2:654841/70‑1 (MQ=255)                                                                                                   cgcgAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTAct                            >  2:262579/1‑133 (MQ=255)                                                                                                   cgcgAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTAct                            <  1:262579/133‑1 (MQ=255)                                                                                                   cgcgAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCg                      <  2:534765/139‑1 (MQ=255)                                                                                                         tGCTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGAt                >  1:1371936/1‑139 (MQ=255)                                                                                                           cTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGt                                                                             >  2:1472247/1‑76 (MQ=255)                                                                                                           cTG‑AAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGt                                                                             <  1:1472247/76‑1 (MQ=255)                                                                                                               aaaTCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAaca                                             <  2:770412/105‑1 (MQ=255)                                                                                                               aaaTCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAaca                                             >  1:770412/1‑105 (MQ=255)                                                                                                                        tGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGATGTGAAGGCGAACtt  <  2:1535892/139‑1 (MQ=255)                                                                                                                           cAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTAct                            >  2:258504/1‑110 (MQ=255)                                                                                                                           cAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTAAct                            <  1:258504/110‑1 (MQ=255)                                                                                                                              ccTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTg                                                                        <  2:253565/63‑1 (MQ=255)                                                                                                                              ccTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTg                                                                        >  1:253565/1‑63 (MQ=255)                                                                                                                                         |                                                                                                                          GCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTG‑AAATCACGCTGGCAGCCTCGAAGGATCCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGATGTGAAGGCGAACTT  >  NC_000913/2049201‑2049457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑