Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,508,454	Δ1 bp	coding (137/879 nt)	php ←	phosphotriesterase homology protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,508,451	0	C	.	100.0%	231.0 / NA	55	coding (140/879 nt)	php	phosphotriesterase homology protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (30/25);  total (30/25)

TGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGCCCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAA  >  NC_000913/3508316‑3508583                                                                                                                                        |                                                                                                                                     tGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGcccg                                                                                                                                   <  1:380259/139‑2 (MQ=255)                              aCCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCaaa                                                                                                     >  2:181967/1‑139 (MQ=255)                                  gTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGtcatc                                                                                                                           <  2:945481/113‑1 (MQ=255)                                  gTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGtcatc                                                                                                                           >  1:945481/1‑113 (MQ=255)                                  gTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCaaa                                                                                                     >  2:425199/1‑135 (MQ=255)                                  gTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCaaa                                                                                                     <  1:425199/135‑1 (MQ=255)                                  gTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGaa                                                                                                 >  2:1000003/1‑139 (MQ=255)                                     cAGGCCACCACGTTGGTCCCCGTCTCGCGCATTCCAGCAAGCATAAATTGCCCATTGCGCCCCATGTAACGGTTGGTCAACACAATCACCTTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGc                                                                                              <  1:224529/139‑1 (MQ=255)                                       ggCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGa                                                                                                  >  2:1023830/1‑133 (MQ=255)                                       ggCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGa                                                                                                  <  1:1023830/133‑1 (MQ=255)                                        gCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATa                                                                                           >  1:241841/1‑139 (MQ=255)                                        gCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACACACG‑CCCGGGTCATCAGGTCGTTCCTCTCCTGGCAAATGAACGCATa                                                                                           >  2:121093/1‑139 (MQ=255)                                             cacGTTGATCCCCGTCTCGCGCATTACATCAAGCATAACTTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGAt                                                                                      >  2:70860/1‑139 (MQ=255)                                             cacGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGAt                                                                                      >  1:467943/1‑139 (MQ=255)                                                           tctcGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTcc                                                                        >  1:1008070/1‑139 (MQ=255)                                                              cgcgCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACg                                                                     >  2:51126/1‑139 (MQ=255)                                                                     tACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGtttt                                                              >  1:249205/1‑139 (MQ=255)                                                                          cAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAg                                                         <  2:249205/139‑1 (MQ=255)                                                                           aaGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAggcggc                                                                             >  1:383838/1‑118 (MQ=255)                                                                           aaGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAggcggc                                                                             <  2:383838/118‑1 (MQ=255)                                                                             gCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc                                                                                                <  1:1055151/97‑1 (MQ=255)                                                                             gCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc                                                                                                >  2:1055151/1‑97 (MQ=255)                                                                             gCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCg                                                      <  2:241841/139‑1 (MQ=255)                                                                              cATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCgg                                                     <  2:328148/139‑1 (MQ=255)                                                                                tAAATTCCTCATTGCTCACCTCGTTACGGTTACTCATCTCAACCACAATCCGCGCG‑CTAGGGTCATCAGCTCGTTCATATCCTAGCAAATGAACGCATACTGTTCAAGGCGGCAGTCCACGTTGTTTTTAAAGGCGgac                                                   <  2:467943/139‑2 (MQ=255)                                                                                 aaaTTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGgaga                                                  <  1:696830/139‑1 (MQ=255)                                                                                 aaaTTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGgaga                                                  <  2:301913/139‑1 (MQ=255)                                                                                 aaaTTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGGTTTTAAAGCCGgaga                                                  <  2:705802/139‑1 (MQ=255)                                                                                 aaaTTGCGCATTGCGCCCCATGTAACGGTTGGTCATCCCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATAGTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGgaga                                                  >  1:705802/1‑139 (MQ=255)                                                                                  aaTTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGgagag                                                 >  2:800776/1‑139 (MQ=255)                                                                                  aaTTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGgagag                                                 >  2:126076/1‑139 (MQ=255)                                                                                   attgcgcattgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGgagaga                                                <  1:1000003/139‑1 (MQ=255)                                                                                       cgcattgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc                                                                                                <  1:298579/87‑1 (MQ=255)                                                                                       cgcattgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAAc                                                                                                >  2:298579/1‑87 (MQ=255)                                                                                       cgcattgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCaa                                            >  1:525102/1‑139 (MQ=255)                                                                                        gcattgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAat                                           >  2:308619/1‑139 (MQ=255)                                                                                        gcattgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAat                                           >  1:43297/1‑139 (MQ=255)                                                                                         cattgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAata                                          >  1:434103/1‑139 (MQ=255)                                                                                         cattgcgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAata                                          <  2:525102/139‑1 (MQ=255)                                                                                              cgcCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAg                                     >  1:1051003/1‑139 (MQ=255)                                                                                                     tGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAggcggc                                                                             <  1:942767/92‑1 (MQ=255)                                                                                                     tGTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAggcggc                                                                             >  2:942767/1‑92 (MQ=255)                                                                                                      gTAACGGTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCAt                             >  1:851350/1‑139 (MQ=255)                                                                                                           ggTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCg                        <  1:70860/139‑1 (MQ=255)                                                                                                            gTTGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGa                       <  2:164637/139‑1 (MQ=255)                                                                                                             ttGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGga                                                    <  2:436017/109‑1 (MQ=255)                                                                                                             ttGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGga                                                    >  1:436017/1‑109 (MQ=255)                                                                                                              tGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCAtct                                                                                                              >  1:249901/1‑50 (MQ=39)                                                                                                              tGGTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCAtct                                                                                                              <  2:249901/50‑1 (MQ=39)                                                                                                               ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCa                                                                                             <  2:447206/66‑1 (MQ=255)                                                                                                               ggTCATCTCAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCa                                                                                             >  1:447206/1‑66 (MQ=255)                                                                                                                       cAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCa                                                                                             >  2:796024/1‑58 (MQ=255)                                                                                                                       cAATCACATTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCa                                                                                             <  1:796024/58‑1 (MQ=255)                                                                                                                             caTTACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGa      <  2:851350/139‑1 (MQ=255)                                                                                                                               ttACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCg                        >  2:384019/1‑119 (MQ=255)                                                                                                                               ttACGCACG‑CCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCg                        <  1:384019/119‑1 (MQ=255)                                                                                                                                 acgcacg‑cCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCaa  <  1:800776/139‑1 (MQ=255)                                                                                                                                  cgcacg‑cCCGGGTCATCAGGTCGTTCATCTCCTGGCaaa                                                                                                     >  1:785639/1‑39 (MQ=255)                                                                                                                                  cgcacg‑cCCGGGTCATCAGGTCGTTCATCTCCTGGCaaa                                                                                                     <  2:785639/39‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     TGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGCCCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAA  >  NC_000913/3508316‑3508583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑