BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,344,636	(G)_5→6	intergenic (‑27/+121)	yciZ ← / ← gmr	uncharacterized protein/cyclic‑di‑GMP phosphodiesterase, csgD regulator, modulator of RNase II stability

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,344,631	1	.	G	98.0%	521.5 / 1.3	146	intergenic (‑22/+126)	yciZ/gmr	uncharacterized protein/cyclic‑di‑GMP phosphodiesterase, csgD regulator, modulator of RNase II stability
Reads supporting (aligned to +/- strand): ref base . (3/0); new base G (65/78); total (68/78)
Fisher's exact test for biased strand distribution p-value = 9.86e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 159 total aligned reads displayed.

TCTGCTTTAAGTATCTCGAGATTATGGATCGCAGAGTGGTAATCGCCTGCCACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAA‑G‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGC  >  NC_000913/1344497‑1344762

              ctcGAGATTATGGATCGCAGAGTGGTAATCGCCTTCCACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATGAAGGACATCCTTATAGCCAAAG‑G‑GG‑GGAAAATGaataat                                                                                                                    >  2:618663/1‑139 (MQ=255)

                   gaTTATGGATCGCAGAGTGGTAATCGCCTGCCACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCaaa                                                                                                               >  2:128362/1‑139 (MQ=255)

                             cGCAGAGTGGTAATCGCCTGCCACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGc                                                                                                      <  1:893931/138‑1 (MQ=255)

                             cGCAGAGTGGTAATCGCCTGCCACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCt                                                                                                     >  2:982265/1‑139 (MQ=255)

                               cAGAGTGGTAATCGCCTGCCACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGaa                                                                                                                        <  2:394551/118‑1 (MQ=255)

                                  agTGGTAATCGCCTGCCACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAag                                                                                                >  1:255327/1‑139 (MQ=255)

                                            gCCTGCCACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGttt                                                                                      >  1:303512/1‑139 (MQ=255)

                                            gCCTGCCACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGttt                                                                                      >  1:218152/1‑139 (MQ=255)

                                              cTGCCACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGc                                                                                                      <  1:286696/121‑1 (MQ=255)

                                              cTGCCACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGc                                                                                                      >  2:286696/1‑121 (MQ=255)

                                              cTGCCACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTaa                                                                                    >  2:118031/1‑139 (MQ=255)

                                                 ccACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAg                                                                                 >  2:83773/1‑139 (MQ=255)

                                                    caAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCaa                                                                              <  1:118031/139‑1 (MQ=255)

                                                     aaGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAAc                                                                             <  2:303512/139‑1 (MQ=255)

                                                           atCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTaa                                                                       >  2:895210/1‑139 (MQ=255)

                                                              tAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTa                                                                    <  2:445946/139‑1 (MQ=255)

                                                               aaCACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTat                                                                   >  2:190125/1‑139 (MQ=255)

                                                                 cacaATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTatca                                                                 <  1:618663/139‑1 (MQ=255)

                                                                 cacaATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTatca                                                                 >  1:965692/1‑139 (MQ=255)

                                                                  acaATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTatcat                                                                >  1:731475/1‑139 (MQ=255)

                                                                   caATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTatcatc                                                               <  2:255327/139‑1 (MQ=255)

                                                                      tatCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAg                                                            >  2:165327/1‑139 (MQ=255)

                                                                      tatCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAg                                                            >  2:199359/1‑139 (MQ=255)

                                                                      tatCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAg                                                            >  2:37180/1‑139 (MQ=255)

                                                                      tatCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAg                                                            >  1:942747/1‑139 (MQ=255)

                                                                      tatCAATACGTTCGGCAACGCGCTGGGCATCAAAATCAGACATCAAGGACATCCTTATAG‑CACAA‑GTGG‑AGAAATTGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAg                                                            >  2:497567/1‑139 (MQ=255)

                                                                        tCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGa                                                          <  2:942747/139‑1 (MQ=255)

                                                                        tCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGa                                                          <  2:9163/139‑1 (MQ=255)

                                                                         cAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGaa                                                         <  1:757872/139‑1 (MQ=255)

                                                                         cAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGaa                                                         <  1:84611/139‑1 (MQ=255)

                                                                          aaTACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCCTCCAGGAAt                                                        <  1:707492/139‑1 (MQ=255)

                                                                          aaTACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAAt                                                        >  1:516740/1‑139 (MQ=255)

                                                                           aTACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATTCTTATAG‑CAAAA‑G‑GGAGGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGACTAATTATCATCCAGGAATa                                                       >  1:671874/1‑139 (MQ=255)

                                                                           aTACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGa                                                          >  1:1003600/1‑136 (MQ=255)

                                                                           aTACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATa                                                       >  2:32721/1‑139 (MQ=255)

                                                                           aTACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATa                                                       <  2:763339/139‑1 (MQ=255)

                                                                           aTACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATa                                                       <  1:1034931/139‑1 (MQ=255)

                                                                           aTACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATa                                                       <  1:199359/139‑1 (MQ=255)

                                                                            tACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAagag                                                                                              <  1:692791/99‑1 (MQ=255)

                                                                            tACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATAc                                                      >  2:315537/1‑139 (MQ=255)

                                                                            tACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATAc                                                      >  1:1054255/1‑139 (MQ=255)

                                                                            tACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATAc                                                      >  1:1001678/1‑139 (MQ=255)

                                                                             aCGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACg                                                     <  2:595047/139‑1 (MQ=255)

                                                                             aCGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACg                                                     <  2:731475/139‑1 (MQ=255)

                                                                                ttCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGc                                                                                                      <  1:66779/87‑1 (MQ=255)

                                                                                ttCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGc                                                                                                      >  2:66779/1‑87 (MQ=255)

                                                                                ttCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAagag                                                                                              <  1:799021/95‑1 (MQ=255)

                                                                                ttCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGc                                                                          >  2:789496/1‑115 (MQ=255)

                                                                                ttCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGata                                                  >  1:900777/1‑139 (MQ=255)

                                                                                ttCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGata                                                  >  2:480903/1‑139 (MQ=255)

                                                                                ttCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGata                                                  >  1:358246/1‑139 (MQ=255)

                                                                                ttCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGata                                                  >  2:21631/1‑139 (MQ=255)

                                                                                ttCGGCAACGCGCCGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCAAGCACTACGata                                                  >  2:707492/1‑139 (MQ=255)

                                                                                 tCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACg                                                     >  1:683669/1‑135 (MQ=255)

                                                                                 tCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACg                                                     <  2:683669/135‑1 (MQ=255)

                                                                                 tCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGatat                                                 >  1:1019324/1‑139 (MQ=255)

                                                                                  cGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAagag                                                                                              >  1:587284/1‑93 (MQ=255)

                                                                                  cGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACg                                                     >  1:323502/1‑134 (MQ=255)

                                                                                       aCGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATg                                           <  1:165327/139‑1 (MQ=255)

                                                                                        cgcgCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGc                                                                                                      <  1:389961/79‑1 (MQ=255)

                                                                                        cgcgCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTa                                                                                     >  1:308294/1‑96 (MQ=255)

                                                                                        cgcgCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTa                                                                                     <  2:308294/96‑1 (MQ=255)

                                                                                         gcgcTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTatcatc                                                               <  2:137447/117‑1 (MQ=255)

                                                                                          cgcTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACg                                        >  1:170289/1‑139 (MQ=255)

                                                                                          cgcTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACg                                        <  1:423337/139‑1 (MQ=255)

                                                                                          cgcTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACg                                        >  2:1016168/1‑139 (MQ=255)

                                                                                          cgcTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACg                                        <  2:1019324/139‑1 (MQ=255)

                                                                                          cgcTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACg                                        >  2:317449/1‑139 (MQ=255)

                                                                                               ggCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGata                                   <  2:358246/139‑1 (MQ=255)

                                                                                                    cAAATTCAGACATCAAGGACATCCTTCTAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGACTAATTATCATCCAGGAATACGATATATAATGACGGGATATAgcg                              <  2:671874/139‑1 (MQ=255)

                                                                                                    cAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAgcg                              <  1:190125/139‑1 (MQ=255)

                                                                                                    cAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAgcg                              <  1:895210/139‑1 (MQ=255)

                                                                                                    cAAATTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAgcg                              <  1:128362/139‑1 (MQ=255)

                                                                                                      aaTTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCt                            <  2:1001678/139‑1 (MQ=255)

                                                                                                      aaTTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCt                            <  1:824127/139‑1 (MQ=255)

                                                                                                      aaTTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCt                            <  1:223171/139‑1 (MQ=255)

                                                                                                      aaTTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCt                            <  1:317449/139‑1 (MQ=255)

                                                                                                       aTTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTa                           <  1:480903/139‑1 (MQ=255)

                                                                                                       aTTCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTa                           <  1:375078/139‑1 (MQ=255)

                                                                                                         tCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACg                                                     <  1:133124/111‑1 (MQ=255)

                                                                                                         tCAGACATCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGAtata                                 <  1:399057/131‑1 (MQ=255)

                                                                                                               aTCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCt                            <  2:944470/130‑1 (MQ=255)

                                                                                                               aTCAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGtatata                   <  2:396812/139‑1 (MQ=255)

                                                                                                                 cAAGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCt                                                                         >  2:107123/1‑83 (MQ=255)

                                                                                                                   aGGACATCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCaa                                                                              <  2:533404/76‑1 (MQ=255)

                                                                                                                        aTCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCT‑AAAGAGAAGTGTTTAACAGCAACGGCTAATTatcatc                                                               <  2:540790/85‑1 (MQ=255)

                                                                                                                        aTCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCT‑AAAGAGAAGTGTTTAACAGCAACGGCTAATTatcatc                                                               >  1:540790/1‑85 (MQ=255)

                                                                                                                         tCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATa                                                       <  1:105335/93‑1 (MQ=255)

                                                                                                                         tCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATa                                                       >  2:105335/1‑93 (MQ=255)

                                                                                                                         tCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATa                                                       <  1:280370/93‑1 (MQ=255)

                                                                                                                         tCCTTATAG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGtat                      >  1:998934/1‑126 (MQ=255)

                                                                                                                              ataG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTAt    >  1:181505/1‑139 (MQ=255)

                                                                                                                               taG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAACGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATaa                                             >  1:871507/1‑97 (MQ=255)

                                                                                                                               taG‑CAAAAGG‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATaa                                             <  2:871507/97‑1 (MQ=255)

                                                                                                                                    aaag‑G‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACgg                                                                           <  2:921424/62‑1 (MQ=255)

                                                                                                                                    aaag‑G‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGa                                                          >  1:932544/1‑79 (MQ=255)

                                                                                                                                    aaag‑G‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACg                                                     <  1:458746/84‑1 (MQ=255)

                                                                                                                                    aaag‑G‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGata                                   <  2:181433/102‑1 (MQ=255)

                                                                                                                                    aaag‑G‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCt                            <  2:707325/109‑1 (MQ=255)

                                                                                                                                    aaag‑G‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCt                            <  2:128103/109‑1 (MQ=255)

TCTGCTTTAAGTATCTCGAGATTATGGATCGCAGAGTGGTAATCGCCTGCCACAAGGATATCTAACACAATATCAATACGTTCGGCAACGCGCTGGGCATCAAATTCAGACATCAAGGACATCCTTATAG‑CAAAA‑G‑GG‑GGAAAATGAATAATGCAAAAATTGCCGCTAAAAGAGAAGTGTTTAACAGCAACGGCTAATTATCATCCAGGAATACGATATATAATGACGGGATATAGCGCTAAGTATATATATTCATCTACTTATGC  >  NC_000913/1344497‑1344762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`