| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,767,507 | (C)7→8 | intergenic (+152/‑203) | rnlB → / → yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,767,500 | 1 | . | C | 81.1% | 136.1 / 27.1 | 54 | intergenic (+145/‑210) | rnlB/yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
| Reads supporting (aligned to +/- strand): ref base . (6/4); new base C (22/21); total (29/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑C‑C‑CCCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TT‑C‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATA > NC_000913/2767367‑2767631 | ctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TT‑c‑cc‑c < 1:720100/139‑1 (MQ=255)ctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCC‑TCACAACGTCCTGAATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TT‑c‑cc‑c < 2:670825/139‑1 (MQ=255) aCCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TT‑C‑CC‑CCC‑C‑cttta < 1:17165/139‑3 (MQ=255) ttCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑C‑CAACCCC‑TCCCAACGTTCTGTCTAGGGAACAAGGTCTCCCCTGTGCTCCAACGACTACAT‑TT‑C‑CC‑CCCAC‑TTTAACCTGCTCaata > 1:1022695/1‑139 (MQ=255) atTAACTTGTCCAATTTGATCAAATAGCATTAAA‑C‑C‑CGCCC‑TCACAACGACCTGCATAGGAAACACGTTTTACACTATGCACCAACGACTAAAT‑TT‑CTCC‑CCC‑C‑TTTAAACTTCTCCATAATGTCACCAATTTTCTCGa > 2:706418/1‑139 (MQ=255) gTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCC‑TCACAACGTCCTGCATAGGGAACACTATTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTat > 2:423356/1‑139 (MQ=255) tCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TT‑c‑cc‑ccc‑ccttta < 1:975251/99‑6 (MQ=255) tCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TT‑CTCC‑CCC‑C‑TTTa > 2:975251/1‑99 (MQ=255) tCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACACACGACTAAAT‑TT‑C‑CC‑CCC‑CATTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCTTatg > 2:209109/1‑139 (MQ=255) tCCAATATGATCAAATAGCATT‑AA‑CAA‑CACGC‑TCACACCCTCCTCAATAACAAACCCATTTCCCCCTCTGCACCCACCACTAACTATA‑C‑CA‑ACA‑C‑TGTAAACTTCTCAAAAATGTCAACAACCTTATGTAAACCTTatg > 1:411289/1‑139 (MQ=255) accaagctgatgaAGTAGCTTT‑AACC‑C‑CCCCC‑TCACATCGTCCTGCATAGGGAACACGTTTTCCCCGTTGCACCCACGTCTAAGT‑TTCC‑CC‑CCC‑C‑TTTACACCTCTCAATAATGTCTCCAATTTTCTGGAAATCCTatg < 2:621399/132‑1 (MQ=255) aTCAAATAGCATT‑AACC‑C‑CCCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTc < 2:970999/139‑1 (MQ=255) tCAAATAGCATT‑AA‑C‑C‑CCCCCGTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTAAAACTTCCCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTcc > 2:515137/1‑139 (MQ=255) aaTAGCATT‑AATC‑T‑CCCCC‑TCACAACGTCCGCCCCAGGGAACACGTTTTCCCCCGCTCAGCCACGACTAAAT‑TT‑C‑CC‑CCCAC‑TTCAGTCTTCCTAGTCGCGTCTCCAATTTTCTGTTATACCCATGATGCCTCTccctg > 1:391244/1‑137 (MQ=255) aTAGCATT‑AA‑C‑C‑CCCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTa > 2:668324/1‑139 (MQ=255) tAGCATT‑AACC‑C‑CCCCC‑TCACACCGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCg > 2:392623/1‑136 (MQ=255) tAGCATT‑AACC‑C‑CCCCC‑TCACAACGTCCTGCATAGGGAACCCGTTTTCCCCTGTGCACCCACGACTAAAT‑TTAC‑CC‑CCA‑C‑TATAAACTTCTCAATAATGCCACCAATTTTCTGGAAATCCTATGTTTCCTTTACCGGTa > 2:482860/1‑139 (MQ=255) tAGCATT‑AACC‑C‑CCCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCg < 1:392623/136‑1 (MQ=255) tAGCATT‑AACC‑C‑CCCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTa > 2:204340/1‑139 (MQ=255) aGCATT‑AACC‑C‑CCCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACtt < 1:323194/88‑1 (MQ=255) aGCATT‑AACC‑C‑CCCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACtt > 2:323194/1‑88 (MQ=255) aTT‑AACC‑C‑CCCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TT‑c‑cc‑ccc‑ccttta < 1:841527/80‑6 (MQ=255) aTT‑AACC‑C‑CCCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TT‑C‑CC‑CCC‑C‑cttta > 2:841527/1‑78 (MQ=255) attaccc‑c‑ccccc‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATg < 2:132297/133‑1 (MQ=255) ttaacc‑c‑ccccc‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTAACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATgg > 1:540801/6‑139 (MQ=255) c‑ccccc‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCTCCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCtgt < 2:1022695/139‑1 (MQ=255) ccccc‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTTTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCtgtg < 1:558881/139‑1 (MQ=255) cccc‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGCTGCCTTTCCCGGTAAATGGt > 2:53354/1‑132 (MQ=255) cccc‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGt < 1:53354/132‑1 (MQ=255) cccc‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGa > 2:305794/1‑139 (MQ=255) cccc‑TCACAAAGTCCTGCCTAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCCCAATAATGTCAACAATTTTCTGGAAATCCTATGATCCCTTTCCCGGTAAATGGTGCTGTGa > 1:549153/1‑139 (MQ=255) ccc‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAg < 1:482860/139‑1 (MQ=255) aCGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGaaa < 2:540801/139‑1 (MQ=255) tCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGAt > 1:841582/1‑139 (MQ=255) tGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCt < 1:265534/139‑1 (MQ=255) gCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAg < 1:220455/124‑1 (MQ=255) gCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAg > 2:220455/1‑124 (MQ=255) gCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTg < 2:440827/139‑1 (MQ=255) cATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGc > 1:902192/1‑139 (MQ=255) ggAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAAt > 1:110429/1‑107 (MQ=255) ggAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAAt < 2:110429/107‑1 (MQ=255) ggAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCtgtg < 2:182549/116‑1 (MQ=255) ggAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCtgtg > 1:182549/1‑116 (MQ=255) ggAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCt > 1:306223/1‑139 (MQ=255) aaCACGTTTTCCCCTGTGCACCCACGACTAAATCTC‑C‑CC‑CCC‑C‑TGTAAACTTCTCTATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTAt < 2:570018/139‑1 (MQ=255) acGTTTTCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTAtgtg > 2:180028/1‑139 (MQ=255) ttCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGt < 1:663862/100‑1 (MQ=255) ttCCCCTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGt > 2:663862/1‑100 (MQ=255) ccccTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTc < 2:971386/86‑1 (MQ=255) ccccTGTGCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTc > 1:971386/1‑86 (MQ=255) gtgCACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAAt > 1:970616/1‑139 (MQ=255) cACCCACGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCtt < 1:180028/139‑1 (MQ=255) aCGACTAAATGTC‑C‑CC‑CCC‑C‑TTTATACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCagat < 2:156011/139‑1 (MQ=255) aCGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCagat < 1:305794/139‑1 (MQ=255) aCGACTAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCagat < 2:902192/139‑1 (MQ=255) aCGACTAAAT‑TT‑C‑CCGCCC‑C‑TTTAAACTTCTCAATAATGTAACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTTAGAAACATTCTGCAATCTATGTGCATTGAAAAAATCTTCagat < 1:706418/139‑1 (MQ=255) tAAAT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAgatagata > 1:824966/1‑139 (MQ=255) aaT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCag > 2:741273/1‑130 (MQ=255) aaT‑TTCC‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCag < 1:741273/130‑1 (MQ=255) | CTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑C‑C‑CCCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑TT‑C‑CC‑CCC‑C‑TTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATA > NC_000913/2767367‑2767631 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |