| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,330,357 | +T | coding (1395/1434 nt) | dacB → | D‑alanyl‑D‑alanine carboxypeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,330,353 | 1 | . | T | 97.8% | 142.9 / ‑3.5 | 46 | coding (1391/1434 nt) | dacB | D‑alanyl‑D‑alanine carboxypeptidase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (21/24); total (21/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
GCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑G‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATC > NC_000913/3330225‑3330485 | gCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAg < 1:75791/139‑1 (MQ=255) gggtTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt < 2:608057/139‑1 (MQ=255) ggggTATATATCCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCTTTTGTGCAATATTTTTCTTGCTATGCAGTATAACCGGCGGATCAGCTTAATCGCTGTATTCCGTTAGTGCTT‑TTTTGAAAGccat < 2:634899/139‑3 (MQ=255) ggggTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt < 2:490234/139‑1 (MQ=255) ggggTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt < 1:679479/139‑1 (MQ=255) ggggTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTATGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt < 2:816934/139‑1 (MQ=255) ggTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGttt < 2:518970/139‑1 (MQ=255) gTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTg < 2:551553/139‑1 (MQ=255) tatataACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGt < 1:630021/139‑1 (MQ=255) tGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGata < 1:103727/139‑1 (MQ=255) tGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGata < 2:779228/139‑1 (MQ=255) ggCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCATTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGatat > 1:662314/1‑139 (MQ=255) cGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt < 2:582457/123‑1 (MQ=255) cGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGt > 1:582457/1‑123 (MQ=255) cGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATaa > 2:997551/1‑131 (MQ=255) cGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATaa < 1:997551/131‑1 (MQ=255) cGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATAttt > 2:221767/1‑139 (MQ=255) agcgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTaatc < 1:221767/139‑4 (MQ=255) cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCG‑TTGTATAAAGATATTTATCAGAACAATTAATCaaa > 2:181521/1‑139 (MQ=255) agcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAg > 2:697040/1‑124 (MQ=255) agcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAg < 1:697040/124‑1 (MQ=255) agcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCaaaa > 2:856043/1‑139 (MQ=255) gggCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa < 1:2418/139‑1 (MQ=255) cAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGata > 2:12942/1‑110 (MQ=255) cAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGata < 1:12942/110‑1 (MQ=255) cAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAAc > 1:168264/1‑139 (MQ=255) aCGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAAccc < 2:662314/139‑1 (MQ=255) aCGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAAccc < 2:108217/139‑1 (MQ=255) gCGTTTGTGCAATATCTTTCTGGC‑ATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac < 2:852967/137‑1 (MQ=255) gCGTTTGTGCAATATCTTTCTGGC‑ATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac > 1:852967/1‑137 (MQ=255) tgCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCgg > 2:264127/1‑139 (MQ=255) tATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGAc > 1:20022/1‑139 (MQ=255) tATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGAc > 2:89918/1‑139 (MQ=255) tATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATC‑AAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACa > 1:88515/1‑139 (MQ=255) aTGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACa > 1:500539/1‑139 (MQ=255) tGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACaa < 1:30514/139‑1 (MQ=255) ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTCATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAg > 2:911682/1‑131 (MQ=255) ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAg < 1:911682/131‑1 (MQ=255) ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCttt > 2:956062/1‑139 (MQ=255) gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGtt > 1:259085/1‑139 (MQ=255) ggATCAGCGTAATCGCCGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTa > 1:308747/1‑139 (MQ=255) cGTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa > 1:834520/1‑139 (MQ=255) gTATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa < 2:834520/139‑1 (MQ=255) tATTCCGTTAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa < 2:168264/139‑1 (MQ=255) ttAGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTa > 1:151389/1‑139 (MQ=255) aGTGC‑GTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc < 2:259085/139‑1 (MQ=255) | GCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGC‑G‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATC > NC_000913/3330225‑3330485 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |