| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,897,050 | +G | coding (277/666 nt) | yieH → | phosphoenolpyruvate and 6‑phosphogluconate phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,897,046 | 1 | . | G | 96.4% | 196.4 / ‑0.2 | 56 | coding (273/666 nt) | yieH | phosphoenolpyruvate and 6‑phosphogluconate phosphatase |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (28/26); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑G‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCA > NC_000913/3896911‑3897173 | cAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑g‑gg < 2:922674/139‑1 (MQ=255) aGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑g‑gg‑ggc < 2:902857/139‑3 (MQ=255) tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAg > 2:973587/1‑139 (MQ=255) cTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTc < 1:959572/139‑1 (MQ=255) gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCt > 2:739405/1‑139 (MQ=255) gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCt > 1:288156/1‑139 (MQ=255) tATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGAt > 1:101928/1‑139 (MQ=255) tCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCa > 1:583510/1‑139 (MQ=255) tCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCa > 2:620136/1‑139 (MQ=255) gATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACt < 1:227840/139‑1 (MQ=255) tGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa < 2:162179/103‑1 (MQ=255) tGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa > 1:162179/1‑103 (MQ=255) tGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGcc > 1:938384/1‑139 (MQ=255) gTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa < 1:739405/139‑1 (MQ=255) tCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtg > 1:609405/1‑139 (MQ=255) tCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtg < 2:583510/139‑1 (MQ=255) cTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgt > 1:172183/1‑139 (MQ=255) ttGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg < 2:288156/139‑1 (MQ=255) ttGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg < 1:620136/139‑1 (MQ=255) gAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGa > 1:474544/1‑103 (MQ=255) gAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGa < 2:474544/103‑1 (MQ=255) tACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa < 2:920560/82‑1 (MQ=255) tACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa > 1:920560/1‑82 (MQ=255) tACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACgg > 1:940194/1‑139 (MQ=255) tACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACgg > 2:875512/1‑139 (MQ=255) aCGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGc < 2:885132/139‑1 (MQ=255) tAGCGTAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTgcg > 1:696439/1‑111 (MQ=255) tAGCGTAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTgcg < 2:696439/111‑1 (MQ=255) tAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTg > 1:328297/1‑109 (MQ=255) tAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTg < 2:328297/109‑1 (MQ=255) tAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCaa > 1:660847/1‑139 (MQ=255) aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCaaa > 2:594058/1‑139 (MQ=255) gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTgc > 2:614033/1‑108 (MQ=255) gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTgc < 1:614033/108‑1 (MQ=255) gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGc < 1:973891/133‑1 (MQ=255) gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGc > 2:973891/1‑133 (MQ=255) gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAAt > 2:687735/1‑139 (MQ=255) gCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcag < 2:495727/139‑1 (MQ=255) aaCACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGcc < 2:786938/95‑1 (MQ=255) aaCACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGcc > 1:786938/1‑95 (MQ=255) acacGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTc < 1:594058/139‑1 (MQ=255) cacGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCt < 2:132670/139‑1 (MQ=255) tttACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATgg < 1:248932/139‑1 (MQ=255) ttACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑G‑GGAGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATggg < 1:560862/139‑1 (MQ=255) tACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc < 2:322414/139‑1 (MQ=255) gTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGc > 1:914270/1‑139 (MQ=255) tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑g > 1:145934/1‑47 (MQ=255) tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑g < 2:145934/47‑1 (MQ=255) cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGa > 2:97487/1‑139 (MQ=255) cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGa < 1:97487/139‑1 (MQ=255) cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGa < 2:266377/139‑1 (MQ=255) cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGa > 2:241679/1‑139 (MQ=255) cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGa < 2:830534/139‑1 (MQ=255) cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGa > 2:863422/1‑139 (MQ=255) gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa < 2:387779/36‑1 (MQ=255) gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa > 1:387779/1‑36 (MQ=255) gCTCGGCTGTTCGAGTCAGAACTGGAAGCCATCGAA‑GAGG‑GCTGGAGCCCTCCTGTCAGCGATCACCGCGCCAACGTGTGTGGTATCTATCGGCCCAAATAACTAAATGCAGCAATCGATGGGCAAGCTGAATATGTTg > 2:316393/1‑139 (MQ=255) gTTCGATTGAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGTGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACtt < 2:645697/139‑1 (MQ=255) gTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcag < 2:17655/104‑1 (MQ=255) gTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcag > 1:17655/1‑104 (MQ=255) tCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTcc < 2:940194/139‑1 (MQ=255) cGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTccc < 1:241679/139‑1 (MQ=255) ttCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGa > 1:912316/1‑139 (MQ=255) gAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTc > 2:875102/1‑139 (MQ=255) gAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTc > 2:595166/1‑139 (MQ=255) aaGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCa < 1:875512/139‑1 (MQ=255) | CAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑G‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCA > NC_000913/3896911‑3897173 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |