Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,980,957	(G)6→7	coding (71/1386 nt)	yifK →	putative APC family amino acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,980,951	1	.	G	100.0%	178.8 / NA	50	L22R (CTG→CGG)	yifK	putative APC family amino acid transporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (27/23);  total (27/23)

AACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAT  >  NC_000913/3980820‑3981083                                                                                                                                     |                                                                                                                                      aaCACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑ggggggg                                                                                                                               <  2:11171/139‑2 (MQ=255)      tCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCa                                                                                                                           >  2:398188/1‑139 (MQ=255)             aCCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGt                                                                                                                    >  1:540807/1‑139 (MQ=255)             aCCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGt                                                                                                                    >  2:1047682/1‑139 (MQ=255)             aCCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGt                                                                                                                    >  1:1034083/1‑139 (MQ=255)                 aTAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCg                                                                                                                  <  2:460738/137‑1 (MQ=255)                 aTAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCg                                                                                                                  >  1:460738/1‑137 (MQ=255)                 aTAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGc                                                                                                                <  1:376088/139‑1 (MQ=255)                  tAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGcc                                                                                                               >  2:54213/1‑139 (MQ=255)                  tAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGcc                                                                                                               <  1:367991/139‑1 (MQ=255)                      gATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGc                                                                                                                >  1:216183/1‑134 (MQ=255)                      gATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGc                                                                                                                <  2:216183/134‑1 (MQ=255)                        tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTa                                                                                                         >  1:106420/1‑139 (MQ=255)                           ccGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgg                                                                                                      >  2:107706/1‑139 (MQ=255)                               tAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgggg                                                                                                    >  2:921997/1‑137 (MQ=255)                               tAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgggg                                                                                                    <  1:921997/137‑1 (MQ=255)                               tAACACCGGAAAGCATGCAAACACAACACGAGGACTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgc                                                                                                  >  2:595081/1‑139 (MQ=255)                                aaCACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgc                                                                                                  >  2:842978/1‑139 (MQ=255)                                  cacCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc                                                                                               <  1:1047682/139‑1 (MQ=255)                                    ccGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa                                                                                             <  1:136465/139‑1 (MQ=255)                                     cGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAg                                                                                            <  2:536287/139‑1 (MQ=255)                                      ggAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc                                                                                               >  1:203160/1‑135 (MQ=255)                                      ggAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc                                                                                               <  2:203160/135‑1 (MQ=255)                                       gAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAg                                                                                            <  2:369622/137‑1 (MQ=255)                                       gAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAg                                                                                            <  2:369621/137‑1 (MQ=255)                                       gAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAg                                                                                            >  1:369621/1‑137 (MQ=255)                                       gAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAg                                                                                            >  1:369622/1‑137 (MQ=255)                                        aaaGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCTCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAc                                                                                         >  1:669332/1‑139 (MQ=255)                                            cATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACCGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTg                                                                                     >  2:355219/1‑139 (MQ=255)                                               gCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaaa                                                                                  >  1:909293/1‑139 (MQ=255)                                                 aaaCACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTAGTGGGGCCGCCAGTACCCTGAAATg                                                                                >  2:37916/1‑139 (MQ=255)                                                    cacaacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggc                                                                             <  1:881329/139‑1 (MQ=255)                                                         cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                                        >  2:29248/1‑139 (MQ=255)                                                          acGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggcc                                                                       <  1:50337/139‑1 (MQ=255)                                                               gacttATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCg                                                                  <  1:595081/136‑1 (MQ=255)                                                                    aTGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGGCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg                                                             <  2:669332/139‑1 (MQ=255)                                                                    aTGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg                                                             <  1:398188/139‑1 (MQ=255)                                                                     tGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt                                                            <  2:909293/139‑1 (MQ=255)                                                                     tGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt                                                            <  2:496787/139‑1 (MQ=255)                                                                          gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc                                                       >  2:30433/1‑139 (MQ=255)                                                                                      gagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCTGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGcc                                           >  2:848383/1‑139 (MQ=255)                                                                                       agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCg                                          >  1:1050269/1‑139 (MQ=255)                                                                                                 tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGt                                >  1:334011/1‑139 (MQ=255)                                                                                                 tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGt                                <  2:106420/139‑1 (MQ=255)                                                                                                   ggCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCt                              <  2:1034083/139‑1 (MQ=255)                                                                                                   ggCTGGAAGCTCGACATATCGAACTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCt                              <  1:945529/139‑1 (MQ=255)                                                                                                                         aCTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcatca                     >  1:336664/1‑126 (MQ=255)                                                                                                                         aCTCATCGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcatca                     <  2:336664/126‑1 (MQ=255)                                                                                                                               cGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                                        <  1:23311/69‑1 (MQ=255)                                                                                                                               cGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                                        >  2:23311/1‑69 (MQ=255)                                                                                                                               cGCCCTG‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAt  >  1:506401/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                      AACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAT  >  NC_000913/3980820‑3981083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑