Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,764,018	C→T	*123* (TAG→TAA)	sufA ←	Fe‑S cluster assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,764,018	0	C	T	100.0%	194.9 / NA	61	*123* (TAG→TAA)	sufA	Fe‑S cluster assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/31);  total (30/31)

TTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCAGC  >  NC_000913/1763894‑1764147                                                                                                                              |                                                                                                                                   tttATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCtt                                                                                                                    <  2:583817/139‑1 (MQ=255)   tATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTc                                                                                                                  <  2:99136/139‑1 (MQ=255)       cccTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgcca                                                                                                              <  1:11636/139‑1 (MQ=255)         cTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgccaca                                                                                                            >  2:843917/1‑139 (MQ=255)          tttGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑‑TGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgc                                                                                                                >  2:986207/1‑133 (MQ=255)          tttGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑‑TGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgc                                                                                                                <  1:986207/133‑1 (MQ=255)           ttGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgccacagc                                                                                                          <  2:638093/139‑1 (MQ=255)           ttGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgccacagc                                                                                                          >  1:249817/1‑139 (MQ=255)           ttGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgccacagc                                                                                                          >  2:674528/1‑139 (MQ=255)                aGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCAcat                                                                                                     <  2:607166/139‑1 (MQ=255)                  cTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACTTAGTACCGCTTATACCCCAAA‑GCTTTCCCCACAGCCAcattc                                                                                                   >  1:1033096/1‑139 (MQ=255)                     aTCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCAcattcatt                                                                                                >  1:457226/1‑139 (MQ=255)                       cGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCCGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCt                                                                                              <  2:633164/139‑1 (MQ=255)                        ggTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTg                                                                                             <  1:843917/139‑1 (MQ=255)                        ggTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTg                                                                                             <  2:252314/139‑1 (MQ=255)                        cgttggtAAATGGGTGAAGGATTCCTTTTTTTTAATTCAGCGGGCGGCCGTTCCAGTTTTTGCCACCG‑CAATTCTTTAAGTTTTACAGAACATATTACCGCTTATACCCCAAA‑GCTTTCGCAACGGCACCATCCATTCt                                                                                              <  2:821919/133‑1 (MQ=255)                         gTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGGCTTTCGCCACAGCCACATTCATTCTg                                                                                             <  2:604566/139‑1 (MQ=255)                            gCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgcca                                                                                                              <  2:92837/118‑1 (MQ=255)                            gCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgcca                                                                                                              >  1:92837/1‑118 (MQ=255)                            gCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCt                                                                                         >  2:384183/1‑139 (MQ=255)                                    ggTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGtt                                                                                 >  1:595402/1‑139 (MQ=255)                                         a‑gaaTCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGaa                                                                            <  2:206136/139‑1 (MQ=255)                                                  tcttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAat                                                                    <  1:42827/139‑1 (MQ=255)                                                    ttttTAATTCCGCCGGCCGCCCGTCCAAGGTTTTACATCCTCA‑TTTCTTCAGTATTACCAGACCTAGTACCCCTTATACCCCCAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGGTGTGAAATTTGAatat                                                                  <  1:543011/139‑1 (MQ=255)                                                    tttATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTAGGCTTTAGGGTTGTGAAATTTGAatat                                                                  <  1:674528/139‑1 (MQ=255)                                                     ttATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAat                                                                    <  2:1001100/136‑1 (MQ=255)                                                     ttATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAat                                                                    >  1:1001100/1‑136 (MQ=255)                                                      tataATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa                                                                      <  1:803040/133‑1 (MQ=255)                                                      tataATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa                                                                      >  2:803040/1‑133 (MQ=255)                                                       ataATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCt                                                                                              >  1:328815/1‑108 (MQ=255)                                                       ataATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCt                                                                                              <  2:328815/108‑1 (MQ=255)                                                             cAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAg                                                         <  2:756328/139‑1 (MQ=255)                                                             cAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAg                                                         >  1:291764/1‑139 (MQ=255)                                                                cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAggg                                                                                   >  1:337168/1‑110 (MQ=255)                                                                cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAggg                                                                                   <  2:337168/110‑1 (MQ=255)                                                                cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa                                                                      <  2:168222/123‑1 (MQ=255)                                                                cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa                                                                      >  1:168222/1‑123 (MQ=255)                                                                cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTcc                                                      >  2:129615/1‑139 (MQ=255)                                                                cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTcc                                                      >  1:353045/1‑139 (MQ=255)                                                                cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTcc                                                      >  1:856333/1‑139 (MQ=255)                                                                cGGGCCGCCGGTCCAGGTTTTGACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTcc                                                      >  1:838803/1‑139 (MQ=255)                                                                                     gACATCGTCA‑TTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTc                                 >  2:142854/1‑139 (MQ=255)                                                                                         tCGTCA‑TTGATTCAGTATTACGATACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGCCTTCCGTg                             >  2:924011/1‑139 (MQ=255)                                                                                           cgtcattGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgccacagcca                                                                                                        >  2:435475/6‑63 (MQ=255)                                                                                           cgtcattGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgccacagcca                                                                                                        <  1:435475/58‑1 (MQ=255)                                                                                           cgtcattGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGc                            >  2:893683/6‑139 (MQ=255)                                                                                           cgtcattGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAAAGAAATCGACTTCCGTGc                            >  2:562693/6‑139 (MQ=255)                                                                                             tcattGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCa                          <  1:384183/136‑1 (MQ=255)                                                                                                ttGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCa                       <  2:856333/139‑1 (MQ=255)                                                                                                         tATTACGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGc              <  2:595402/139‑1 (MQ=255)                                                                                                             aCGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgccacagc                                                                                                          >  1:1029630/1‑43 (MQ=255)                                                                                                             aCGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgccacagc                                                                                                          >  1:700590/1‑43 (MQ=255)                                                                                                             aCGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgccacagc                                                                                                          <  2:700590/43‑1 (MQ=255)                                                                                                             aCGAGACATAGTACCGCTTATACCCCAAA‑GCTTTCgccacagc                                                                                                          <  2:1029630/43‑1 (MQ=255)                                                                                                               gagaCATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCt        <  2:838803/139‑1 (MQ=255)                                                                                                               gagaCATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCt        <  2:1033096/139‑1 (MQ=255)                                                                                                                 gaCATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTg      >  2:703111/1‑139 (MQ=255)                                                                                                                 gaCATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTg      <  2:291764/139‑1 (MQ=255)                                                                                                                   cATAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCTCTTGCa    >  2:1008335/1‑139 (MQ=255)                                                                                                                     tAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCAGc  >  1:727972/1‑139 (MQ=255)                                                                                                                     tAGTACCGCTTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCAGc  >  1:429735/1‑139 (MQ=255)                                                                                                                              |                                                                                                                                   TTTATCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAA‑GAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAA‑GCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCAGC  >  NC_000913/1763894‑1764147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑