Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	35,373	G→C	intergenic (‑2/+4)	caiE ← / ← caiD	stimulator of CaiD and CaiB enzyme activities/carnitinyl‑CoA dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	35,373	0	G	C	100.0%	193.9 / NA	53	intergenic (‑2/+4)	caiE/caiD	stimulator of CaiD and CaiB enzyme activities/carnitinyl‑CoA dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (21/32);  total (21/32)

CCACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGGTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGATACGCTTCTTCTAC  >  NC_000913/35238‑35508                                                                                                                                         |                                                                                                                                        ccacGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGcttg                                                                                                                                      >  2:454275/1‑139 (MQ=255)  cacGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGcttgt                                                                                                                                     >  2:269403/1‑139 (MQ=255)   acGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGcttgtt                                                                                                                                    <  2:2896/139‑1 (MQ=255)    cGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGATGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTa                                                                                                                                   <  2:670310/139‑1 (MQ=255)     gCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTaa                                                                                                                                  >  2:697576/1‑139 (MQ=255)      cAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAAc                                                                                                                                 <  2:322145/139‑1 (MQ=255)              cGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTc                                                                                                                         >  2:576259/1‑139 (MQ=255)                     gCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCg                                                                                                                  >  2:255762/1‑139 (MQ=255)                        gATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGAt                                                                                                               >  2:528722/1‑139 (MQ=255)                          tGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATcg                                                                                                             <  2:119014/139‑1 (MQ=255)                                        cccACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCaaa                                                                                               <  2:589273/139‑1 (MQ=255)                                           acaATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGc                                                                                            >  1:532383/1‑139 (MQ=255)                                               tCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGc                                                                                        <  2:250637/139‑1 (MQ=255)                                                   aTCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGcc                                                                                    >  2:212672/1‑139 (MQ=255)                                                         aaTCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAg                                                                                         >  1:444007/1‑128 (MQ=255)                                                         aaTCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAg                                                                                         <  2:444007/128‑1 (MQ=255)                                                          aTCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAAt                                                                             <  1:839265/139‑1 (MQ=255)                                                             aaGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGc                                                                          <  2:408053/139‑1 (MQ=255)                                                               gACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCAt                                                                        <  2:751141/139‑1 (MQ=255)                                                               gACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCAt                                                                        <  2:896225/139‑1 (MQ=255)                                                               gACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCAt                                                                        <  2:960262/139‑1 (MQ=255)                                                               gACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCAt                                                                        <  1:697576/139‑1 (MQ=255)                                                                    cACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTcc                                                                   <  1:212672/139‑1 (MQ=255)                                                                    cACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTcc                                                                   <  1:732210/139‑1 (MQ=255)                                                                       tGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGc                                                                                        >  1:891758/1‑116 (MQ=255)                                                                       tGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGc                                                                                        <  2:891758/116‑1 (MQ=255)                                                                       tGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCa                                                                         <  1:636245/130‑1 (MQ=255)                                                                       tGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCa                                                                         >  2:636245/1‑130 (MQ=255)                                                                          gATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGc                                                             <  1:255762/139‑1 (MQ=255)                                                                              gACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTtct                                                                                                      >  2:187836/1‑94 (MQ=255)                                                                              gACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTtct                                                                                                      <  1:187836/94‑1 (MQ=255)                                                                              gACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGcc                                                                                           >  1:746578/1‑105 (MQ=255)                                                                              gACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCg                                                                  >  1:564305/1‑130 (MQ=255)                                                                              gACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCg                                                                  <  2:564305/130‑1 (MQ=255)                                                                              gACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGaa                                                         >  1:555740/1‑139 (MQ=255)                                                                              gACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGaa                                                         >  2:684223/1‑139 (MQ=255)                                                                              gACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGaa                                                         >  2:417091/1‑139 (MQ=255)                                                                              gACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGaa                                                         >  2:536208/1‑139 (MQ=255)                                                                              gACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTGGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGcc                                                                                           <  2:746578/105‑1 (MQ=255)                                                                                   aCGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCttt                                                                                                                          >  1:1039228/1‑69 (MQ=255)                                                                                   aCGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCttt                                                                                                                          <  2:1039228/69‑1 (MQ=255)                                                                                   aCGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGAt                                                    >  2:37348/1‑139 (MQ=255)                                                                                      ccGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGa                                                 <  2:555740/139‑1 (MQ=255)                                                                                       cGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGAt                                                <  1:536208/139‑1 (MQ=255)                                                                                       cGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGAt                                                <  2:794595/139‑1 (MQ=255)                                                                                        gTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATa                                               <  1:526069/139‑1 (MQ=255)                                                                                                     ccGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACAcg                                  <  1:37348/139‑1 (MQ=255)                                                                                                     ccGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACAcg                                  <  1:25960/139‑1 (MQ=255)                                                                                                       ggAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACAcgcc                                >  2:190608/1‑139 (MQ=255)                                                                                                          aTTAA‑CCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCt                             <  1:269403/139‑1 (MQ=255)                                                                                                                    tCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGAt                                                >  1:950949/1‑111 (MQ=255)                                                                                                                    tCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGAt                                                <  2:950949/111‑1 (MQ=255)                                                                                                                        aGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGa                <  1:454275/139‑1 (MQ=255)                                                                                                                        aGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGa                <  1:190608/139‑1 (MQ=255)                                                                                                                           cGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGATAc             >  1:323809/1‑139 (MQ=255)                                                                                                                                      aCGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGATACGCTTCTTCTAc  <  1:755994/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CCACGCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAA‑CCCCTCAAAGGCGTAATAGCTCACGGTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGATACGCTTCTTCTAC  >  NC_000913/35238‑35508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑