| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,240,754 | (C)5→6 | coding (915/1041 nt) | galS ← | galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,240,749 | 1 | . | C | 97.6% | 146.4 / ‑3.2 | 42 | coding (920/1041 nt) | galS | galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (18/23); total (18/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑C‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGA > NC_000913/2240620‑2240880 | gAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTCGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑c‑ccccctgc < 1:554160/139‑5 (MQ=255) aaaGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑c‑ccccctgca > 2:379915/1‑134 (MQ=255) aTCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCg < 2:376907/139‑1 (MQ=255) ttgAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCg > 2:481710/1‑139 (MQ=255) tgAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCg > 2:449665/1‑138 (MQ=255) tgAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCg < 1:449665/138‑1 (MQ=255) ccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATgg > 2:610863/1‑139 (MQ=255) ccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATgg > 1:136358/1‑139 (MQ=255) tatGGTGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAACACAGTGGCTGGCACGAGGATAAATATTGCCTGGTG‑CGCGGCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGAATAACGCa < 2:180995/137‑1 (MQ=255) tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCTTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 2:239142/139‑1 (MQ=255) tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 1:415343/139‑1 (MQ=255) tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 1:379915/139‑1 (MQ=255) tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 2:212969/139‑1 (MQ=255) ttGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTa > 2:336491/1‑139 (MQ=255) ttGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑c‑ccccctgc < 1:187518/81‑5 (MQ=255) ttGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑c‑ccccctgc > 2:187518/1‑77 (MQ=255) tGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGcc > 2:692359/1‑86 (MQ=255) tGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGcc < 1:692359/86‑1 (MQ=255) gACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTg < 2:145778/139‑1 (MQ=255) gAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATTAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCggg > 1:559565/1‑139 (MQ=255) aTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTc < 2:3557/139‑1 (MQ=255) aTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTc < 1:610863/139‑1 (MQ=255) aTGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTc < 2:136358/139‑1 (MQ=255) aCGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCg > 2:808173/1‑134 (MQ=255) aCGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCg < 1:808173/134‑1 (MQ=255) cGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTaa < 1:509327/139‑1 (MQ=255) cACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACg > 2:671214/1‑139 (MQ=255) tCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATgg < 1:336491/139‑1 (MQ=255) aTAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCg < 2:557480/74‑1 (MQ=255) aTAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCg > 1:557480/1‑74 (MQ=255) aaaaCAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa > 2:285476/1‑96 (MQ=255) aaaaCAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 1:285476/96‑1 (MQ=255) cAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCg < 1:481710/139‑1 (MQ=255) tggctggcACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATCGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGaaa > 2:770212/1‑139 (MQ=255) ctggcACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCg < 2:277187/139‑1 (MQ=255) ggcACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCa < 2:710758/71‑1 (MQ=255) ggcACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCa > 1:710758/1‑71 (MQ=255) cACGAGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCgatga > 1:274476/1‑139 (MQ=255) gaaacagaaTATTCCCTGCCGCC‑CCCCTGCTAGGCGTGTGCGGTGTATAATTTCGCCATTGAAGCAATGGGATATCGGAAGGTCGTTAATTGAGGGTAGGTGTGACAGGCTATGGGAATATCATGGAAACCGATGATTg < 2:184042/132‑1 (MQ=255) aGGATCAATATTGCCTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTga < 1:671214/139‑1 (MQ=255) ccTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 1:679399/65‑1 (MQ=255) ccTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa > 2:679399/1‑65 (MQ=255) ccTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGgt > 2:175259/1‑87 (MQ=255) ccTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGgt < 1:175259/87‑1 (MQ=255) ccTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCga > 2:555911/1‑119 (MQ=255) ccTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCga < 1:555911/119‑1 (MQ=255) ccTGCTGCC‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGa > 2:185904/1‑139 (MQ=255) | GAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑C‑CCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGA > NC_000913/2240620‑2240880 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |