| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,980,957 | (G)6→7 | coding (71/1386 nt) | yifK → | putative APC family amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,980,951 | 1 | . | G | 97.4% | 136.9 / ‑3.4 | 39 | coding (65/1386 nt) | yifK | putative APC family amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (19/19); total (19/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
AACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑GGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCA > NC_000913/3980820‑3981061 | aaCACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gggg‑ggg < 1:608918/139‑2 (MQ=255) aTAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACTCGAGGATTTATGGCAGATAACAAACCAGAGCTACAGTGTGGGCTGGAAGCTCGACATATCGGACACATCGCCCT‑GGGGTTGCACCATTGGCGTCGGc < 2:10119/139‑1 (MQ=255) gATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGtt > 2:248056/1‑139 (MQ=255) ggTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATgggg < 1:248056/139‑1 (MQ=255) acacCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGgccg > 1:29329/1‑139 (MQ=255) cacCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc < 2:29329/139‑1 (MQ=255) cacCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc < 2:246312/139‑1 (MQ=255) cGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAg > 1:214591/1‑139 (MQ=255) aaGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc < 2:214591/139‑1 (MQ=255) caacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa > 2:356318/1‑121 (MQ=255) caacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa < 1:356318/121‑1 (MQ=255) aacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGTCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATggggcgg < 2:539348/139‑1 (MQ=255) aacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgg < 2:363591/139‑1 (MQ=255) acacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccggg > 2:732796/1‑139 (MQ=255) cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGc < 1:56054/99‑1 (MQ=255) cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGc > 2:56054/1‑99 (MQ=255) cAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg > 2:289366/1‑63 (MQ=255) cAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg < 1:289366/63‑1 (MQ=255) ccAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATatcatc > 1:539748/1‑139 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGc < 1:77219/59‑1 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGc > 2:77219/1‑59 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa > 1:248377/1‑78 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa < 2:248377/78‑1 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gggg‑ggg > 2:368953/1‑42 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gggg‑ggg < 1:368953/43‑2 (MQ=255) gCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTc < 1:507602/53‑1 (MQ=255) gCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTc > 2:507602/1‑53 (MQ=255) gAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGgccgcc > 1:819797/1‑70 (MQ=255) gAAGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGgccgcc < 2:819797/70‑1 (MQ=255) aaGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGc > 2:414948/1‑51 (MQ=255) aaGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGc < 1:414948/51‑1 (MQ=255) aaGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggc > 1:25673/1‑86 (MQ=255) aaGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggc < 2:25673/86‑1 (MQ=255) aaGCTCGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtca > 1:397725/1‑139 (MQ=255) cGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa > 1:796258/1‑65 (MQ=255) cGACATATCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa < 2:796258/65‑1 (MQ=255) tCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaaa < 2:748457/69‑1 (MQ=255) tCGAACTCATCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaaa > 1:748457/1‑69 (MQ=255) aTCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa > 2:33581/1‑50 (MQ=255) aTCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa < 1:33581/50‑1 (MQ=255) aTCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc > 1:96003/1‑55 (MQ=255) aTCGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc < 2:96003/55‑1 (MQ=255) cGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATatca > 2:334157/1‑93 (MQ=255) cGCCCTGGGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATatca < 1:334157/93‑1 (MQ=255) | AACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑GGGG‑GGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCA > NC_000913/3980820‑3981061 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |