| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,474,138 | (A)6→7 | coding (15/594 nt) | bdcR → | transcriptional repressor for divergent bdcA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,474,132 | 1 | . | A | 97.0% | 98.1 / ‑3.2 | 33 | coding (9/594 nt) | bdcR | transcriptional repressor for divergent bdcA |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (15/17); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
GCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑A‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCT > NC_000913/4473995‑4474260 | gCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑a > 1:591999/1‑139 (MQ=255) gACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAA‑AAAAACAAtctc < 2:591999/139‑1 (MQ=255) aCTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAA‑AAAAACAATCTcg < 2:3705/139‑1 (MQ=255) cgcCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAgg > 1:507160/1‑139 (MQ=255) cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAA‑AAAAACAATCTCGCGTTCCAgg < 2:510023/95‑1 (MQ=255) cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa > 1:586792/1‑139 (MQ=255) cTTGATTTATTATGCAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑ACAAAAACAATCTCGCGTTCCAgg > 1:510023/1‑95 (MQ=255) tGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaaaa > 1:66884/1‑139 (MQ=255) tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCAc > 2:62083/1‑139 (MQ=255) cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAA‑AAAAACAATCTcg > 2:79420/1‑69 (MQ=255) cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAA‑AAAAACAATCTcg < 1:79420/69‑1 (MQ=255) cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAA‑AAAAACAATCTCGCGTTCCAGgt < 1:489310/79‑1 (MQ=255) cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAA‑AAAAACAATCTCGCGTTCCAGgt > 2:489310/1‑79 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGTTTTTGTAATGGTCACTAA‑AAAAACAATCTCGCGTTCCAGgt < 1:834660/77‑1 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATTGTCACTAA‑AAAAACAATCTCGCGTTCCAGgt > 2:834660/1‑77 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa < 2:80345/120‑1 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa > 1:80345/1‑120 (MQ=255) tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa > 1:222855/1‑139 (MQ=255) aaaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAA‑AAAAACAATCTCGCGtt < 2:402658/63‑1 (MQ=255) aaaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAA‑AAAAACAATCTCGCGtt > 1:402658/1‑63 (MQ=255) aaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGAt < 2:586792/139‑1 (MQ=255) aGGTTTTGTAATGGTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTAc < 2:522999/139‑1 (MQ=255) ggTTTTGTAATGGTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACt < 2:222855/139‑1 (MQ=255) gTTTTGTAATGGTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGc < 1:78017/71‑1 (MQ=255) gTTTTGTAATGGTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGc > 2:78017/1‑71 (MQ=255) ttGTAATGGTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGAt < 2:329494/111‑1 (MQ=255) ttGTAATGGTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGAt > 1:329494/1‑111 (MQ=255) aaTGGTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCt < 2:364102/139‑1 (MQ=255) ggTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg > 2:20764/1‑56 (MQ=255) ggTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg < 1:20764/56‑1 (MQ=255) ggTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCAc < 2:49520/89‑1 (MQ=255) ggTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCAc > 1:49520/1‑89 (MQ=255) gTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGa < 2:521199/102‑1 (MQ=255) gTCACTAA‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGa > 1:521199/1‑102 (MQ=255) | GCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑A‑AAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCT > NC_000913/4473995‑4474260 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |