| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,728,110 | 1 | . | C | 35.9% | 43.9 / 84.2 | 53 | coding (1262/1455 nt) | xylB | xylulokinase |
| Reads supporting (aligned to +/- strand): ref base . (17/17); new base C (8/11); total (25/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGC > NC_000913/3727979‑3728245 | aaGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑ccccccc < 1:244649/139‑1 (MQ=255) cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑cc > 2:300231/1‑131 (MQ=255) cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑cc < 1:300231/131‑1 (MQ=255) cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTAc > 1:821471/1‑128 (MQ=255) cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTAc < 2:821471/128‑1 (MQ=255) aTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTg < 2:11331/139‑1 (MQ=255) tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑cccccccccgta > 1:483342/1‑111 (MQ=255) tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑cccccccccgta < 2:483342/115‑5 (MQ=255) gCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGACCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGa < 2:579820/139‑1 (MQ=255) cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTATTCGAGCTGCTGACCGCTGata > 1:493393/1‑139 (MQ=255) aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGatat > 2:631499/1‑139 (MQ=255) aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATc > 2:485160/1‑139 (MQ=255) ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCTAGCTGCTGACCGCTGATATCCGcc < 2:567619/139‑1 (MQ=255) aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa < 2:491556/139‑1 (MQ=255) aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa > 2:125683/1‑139 (MQ=255) aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAc > 2:715659/1‑139 (MQ=255) aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAc > 1:680738/1‑139 (MQ=255) gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg > 2:516879/1‑139 (MQ=255) ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc < 1:819891/139‑1 (MQ=255) gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa < 2:493393/139‑1 (MQ=255) cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa < 1:125683/139‑1 (MQ=255) cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCCCCGTACGGTAAACGAGCTGCCGCCCGCTGATATCCGCCCGCATCTGACGCCa > 2:614694/1‑139 (MQ=255) ggCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAg < 1:581456/139‑1 (MQ=255) aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcgc < 1:715659/139‑1 (MQ=255) gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc > 1:452632/1‑139 (MQ=255) gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGc < 1:332956/95‑1 (MQ=255) gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGc > 2:332956/1‑95 (MQ=255) gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt > 2:780854/1‑112 (MQ=255) gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt < 1:780854/112‑1 (MQ=255) gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt < 1:816517/98‑1 (MQ=255) gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt > 2:816517/1‑98 (MQ=255) gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAaca < 2:317641/139‑1 (MQ=255) tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg < 1:485160/139‑1 (MQ=255) cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg < 1:614694/139‑1 (MQ=255) tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAgctg > 1:655281/1‑62 (MQ=255) tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAgctg < 2:655281/62‑1 (MQ=255) cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGc > 2:697154/1‑78 (MQ=255) cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGc < 1:697154/78‑1 (MQ=255) aGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg < 1:241647/131‑1 (MQ=255) aGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg > 2:241647/1‑131 (MQ=255) gCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt > 1:727043/1‑80 (MQ=255) gCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt < 2:727043/80‑1 (MQ=255) ccTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCg > 2:8430/1‑131 (MQ=255) ccTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCg < 1:8430/131‑1 (MQ=255) gctgcGCCCAGTGCTGGCCCCACA‑TCCCCCCCCCGTACGGTAATCGAGCTGCTGCCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACCCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg > 2:86860/1‑139 (MQ=255) gctgcGCCCAGTGCTGGCCCCACA‑TCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg < 2:442106/139‑1 (MQ=255) cccAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGc > 2:336465/1‑52 (MQ=255) cccAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGc < 1:336465/52‑1 (MQ=255) ccAGTGCTGGCCCCACA‑TCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCAt < 1:631499/139‑1 (MQ=255) aGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc > 1:750657/1‑72 (MQ=255) aGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc < 2:750657/72‑1 (MQ=255) tGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg < 2:693158/69‑1 (MQ=255) tGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg > 1:693158/1‑69 (MQ=255) tGGCCCCACA‑TCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACg > 2:252323/1‑137 (MQ=255) tGGCCCCACA‑TCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACg < 1:252323/137‑1 (MQ=255) ccccACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCACCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTTCGGTTTAATACCCCAGGCATGCACGACATcc > 1:176533/1‑139 (MQ=255) cccACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa < 2:176533/139‑1 (MQ=255) aca‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGc > 1:243622/1‑139 (MQ=255) | AAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGC > NC_000913/3727979‑3728245 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |