Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,824,491	C→T	*362* (TAG→TAA)	mltB ←	membrane‑bound lytic murein transglycosylase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,824,491	0	C	T	100.0%	38.5 / NA	14	*362* (TAG→TAA)	mltB	membrane‑bound lytic murein transglycosylase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/7);  total (7/7)

TCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑C‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACAT  >  NC_000913/2824393‑2824624                                                                                                     |                                                                                                                                      tCGTCAGGAAGCTGGTGCTGGCCTTGCAATTTTACGAGGAGGATTCAGAAACAAGCTGATTAGCCAGAGGGAAGCTCACG‑CTCCCCCCCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGcc                                                                                               >  2:419058/1‑139 (MQ=255)       ggAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCg                                                                                         <  1:742800/139‑1 (MQ=255)                tgtTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGt                                                                                >  2:653082/1‑139 (MQ=255)                          gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGTTACT‑TACTCgcgccagcgcc                                                                                                                  <  1:142769/95‑1 (MQ=255)                          gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGTTACT‑TACTCgcgccagcgcc                                                                                                                  >  2:142769/1‑95 (MQ=255)                             aTTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCtgtg                                                                   <  1:713897/139‑1 (MQ=255)                                           aTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                                                     <  1:414363/139‑1 (MQ=255)                                                                ccAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGAc                                <  1:653082/139‑1 (MQ=255)                                                                ccAGAGGGAAGCTCACGCC‑CCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGAc                                >  1:815787/1‑139 (MQ=255)                                                                     gggAAGCTCACGCC‑CCCCCTCTTGTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCGAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAc                           <  1:615917/139‑1 (MQ=255)                                                                         aGCTCACGCC‑CCCCCTCTTGTAAATAGTTACT‑TACTCgcgc                                                                                                                         <  1:157305/41‑1 (MQ=255)                                                                         aGCTCACGCC‑CCCCCTCTTGTAAATAGTTACT‑TACTCgcgc                                                                                                                         >  2:157305/1‑41 (MQ=255)                                                                                             gTAAATAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAAc    >  2:32784/1‑139 (MQ=255)                                                                                               aaaTAGTTACT‑TACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACAt  >  2:309394/1‑139 (MQ=255)                                                                                                     |                                                                                                                                      TCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACG‑C‑CCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACAT  >  NC_000913/2824393‑2824624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑