Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,171,879	C→T	*111* (TAG→TAA)	ygiZ ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,171,879	0	C	T	100.0%	113.4 / NA	36	*111* (TAG→TAA)	ygiZ	inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/22);  total (14/22)

TGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAAT‑GCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑CCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAA  >  NC_000913/3171746‑3172006                                                                                                                                       |                                                                                                                                 tGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAAT‑GCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTaa                                                                                                                               <  2:725043/137‑1 (MQ=255) tGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAAT‑GCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTaa                                                                                                                               >  1:725043/1‑137 (MQ=255)    ccAGCGAATTGCCAAAATTGATGGCAATAATGGTTGATTTGGGAATGGCGGACAAAGTTGATTTTGAGG‑GAAGGTTAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCa                                                                                                                          <  1:299483/139‑1 (MQ=255)    ccAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAAT‑GCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCa                                                                                                                          <  2:333942/139‑1 (MQ=255)     cAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAAT‑GCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCaa                                                                                                                         <  2:636770/139‑1 (MQ=255)                   aTTGCATGGCATGAATGTTGAATTTGGGAAT‑GCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTcc                                                                                                           <  2:522835/139‑1 (MQ=255)                                           tGGGAAT‑GCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                   <  2:791128/139‑1 (MQ=255)                                              gAAT‑GCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGc                                                                                                                           <  1:276590/96‑1 (MQ=255)                                              gAAT‑GCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGc                                                                                                                           >  2:276590/1‑96 (MQ=255)                                                          aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccga                                                                    <  2:47011/139‑1 (MQ=255)                                                           aGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccgag                                                                   <  1:109163/139‑1 (MQ=255)                                                           aGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccgag                                                                   <  1:592679/139‑1 (MQ=255)                                                            ggTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCaaaaaa                                                                                                   >  1:812965/1‑107 (MQ=255)                                                            ggTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCaaaaaa                                                                                                   <  2:812965/107‑1 (MQ=255)                                                                 aTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                   >  1:9491/1‑118 (MQ=255)                                                                 aTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                   <  2:9491/118‑1 (MQ=255)                                                                                                tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccgag                                                                   >  1:36142/1‑102 (MQ=255)                                                                                                tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccgag                                                                   <  2:36142/102‑1 (MQ=255)                                                                                                tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑CCGAGCGAGAATACATATACCCAAAACTCGAACATTTcc                                 <  1:75517/136‑1 (MQ=255)                                                                                                tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑CCGAGCGAGAATACATATACCCAAAACTCGAACATTTcc                                 >  2:75517/1‑136 (MQ=255)                                                                                                       gagaGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccgag                                                                   >  2:674218/1‑95 (MQ=255)                                                                                                       gagaGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccgag                                                                   <  1:674218/95‑1 (MQ=255)                                                                                                            ccattAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCAAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccg                                                                     >  2:485337/4‑88 (MQ=255)                                                                                                            ccGTTAAAACGACTCTCATAGATTTTATTAATAGCaa                                                                                                                         <  1:354714/37‑1 (MQ=255)                                                                                                            ccGTTAAAACGACTCTCATAGATTTTATTAATAGCaa                                                                                                                         >  2:354714/1‑37 (MQ=255)                                                                                                            ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                   <  1:262776/75‑1 (MQ=255)                                                                                                            ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                   >  2:262776/1‑75 (MQ=255)                                                                                                            ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccgagcgag                                                               <  1:367563/94‑1 (MQ=255)                                                                                                            ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccgagcgag                                                               >  2:367563/1‑94 (MQ=255)                                                                                                            ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑Ccg                                                                     <  1:485337/88‑1 (MQ=255)                                                                                                            ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑CCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAagag                      >  1:151013/1‑135 (MQ=255)                                                                                                            ccGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑CCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAagag                      <  2:151013/135‑1 (MQ=255)                                                                                                              gTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                 >  2:92003/1‑139 (MQ=255)                                                                                                               ttAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑CCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt               <  2:167220/139‑1 (MQ=255)                                                                                                                     cGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagat          <  1:92003/139‑1 (MQ=255)                                                                                                                            cATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑CCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaataa  >  1:567168/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                 TGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAAT‑GCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATA‑CCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAA  >  NC_000913/3171746‑3172006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑