| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,767,445 | (C)7→8 | intergenic (+90/‑265) | rnlB → / → yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,767,438 | 1 | . | C | 92.6% | 74.6 / 0.2 | 28 | intergenic (+83/‑272) | rnlB/yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (12/13); total (14/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01 | |||||||||||
CTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑A‑A‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCC > NC_000913/2767306‑2767561 | ctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAATCGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑A‑A‑caccac > 1:688995/1‑137 (MQ=255) cccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑A‑ACCCCCCCCTCACAACGt > 2:920065/1‑139 (MQ=255) cccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑A‑A‑cccccccaccacacgcc > 2:836009/1‑129 (MQ=255) ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑A‑ACCCCCCCCTCACAACGTCCTGCATAGGGAac < 1:920065/139‑1 (MQ=255) aGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑A‑ACCCCCCCCTCACAACGTCCTGCATAGGGAac < 2:189435/136‑1 (MQ=255) aGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑A‑ACCCCCCCCTCACAACGTCCTGCATAGGGAac > 1:189435/1‑136 (MQ=255) tctatgtatgtctGAAACTCTTCTTTCTAAACGAACCAGTATAGACTTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑ATC‑CCCCCCCTCACGGCGTCCTGTATAGGGAACACGTTTTccc < 1:760930/127‑1 (MQ=255) ggATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑A‑A‑cccccccctca > 2:505456/1‑100 (MQ=255) ggATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑A‑A‑cccccccctca < 1:505456/104‑5 (MQ=255) gtataagagacaggTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑A‑ACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTccc < 2:522117/126‑1 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑A‑CACCCAATCAAAACGTCATACATAGGGAACACGTTTAACCCTGTGCACCACAGAATAAATTTCCACCCCTTTAAACTTATCaataatat > 2:706026/1‑137 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑A‑ATCCCCCCCTCACAACGTCCTGCTTAGAGAACACGTTTTCCGCTGTTTAGACACCACTGAATATCCCCCCCGTCAAACTTCTCACTAATGt > 1:710968/1‑139 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑A‑ACCCCCCCCTCTCAACTTCCTGCATAGGGAACAACTTTTCCCCTGTGCACACACGACTAAATTTCCCCCACTTCAAAccagacacttatca > 1:522117/1‑126 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑A‑ACCCCCCCCTCACAACGt < 2:227866/66‑1 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑A‑ACCCCCCCCTCACAACGt > 1:227866/1‑66 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑A‑ACCCCCCCCTCACAACGTCCTGCATAGGGAac < 1:65422/80‑1 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑A‑ACCCCCCCCTCACAACGTCCTGCATAGGGAac > 2:65422/1‑80 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑A‑ACCCCCCCCTCACAACGTCCTGCAAAGGGAACTAGTTTTCCCTTGTGCACCCACGACTAAATTTCCTCCCCTTTAAACTTCCCAATAATga > 1:883918/1‑138 (MQ=255) tGATATTAACTTGTCCAATATGATCAAATAGCATT‑A‑ACCCCCCCCTCCCAACGTCCTGCATAGGGAACACGGTTGCGCCTCTGCACCCAAGACTAAATTACTCACCCTTTAAAATTCTGAATAATATCACCATTTTTCt > 2:757195/1‑139 (MQ=255) atatTAACTTGTCCAATATGATCAAATAGCATT‑A‑ACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTgg < 2:710968/139‑1 (MQ=255) atatTAACTTGTCCAATATGATCAAATAGCATT‑A‑ACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTgg < 2:377948/139‑1 (MQ=255) atatTAACTTGTCCAATATGATCAAATAGCATT‑A‑ACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTgg < 2:883918/139‑1 (MQ=255) atTAACTTGTCCAATATGATCAAATAGCATT‑A‑ACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGaa < 1:706026/139‑1 (MQ=255) tatGATCAAATAGCATT‑A‑ACCCCCCCCTCACAACGt > 2:213368/1‑36 (MQ=255) tatGATCAAATAGCATT‑A‑ACCCCCCCCTCACAACGt < 1:213368/36‑1 (MQ=255) tGATCAAATAGCATT‑A‑ACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTACACCCAAGACTAAATTTCCCCCCCTTTAAACTTCTCAAAAATGACACCAATTTTCTGGAAATCCTATGATGTCttt > 2:173043/1‑139 (MQ=255) gaccAAATAGCATT‑A‑C‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTcc < 1:836009/136‑1 (MQ=255) aTT‑A‑ACCCCCCCCTCACAACGTCCTGCATAGGGAac < 2:324550/36‑1 (MQ=255) aTT‑A‑ACCCCCCCCTCACAACGTCCTGCATAGGGAac > 1:324550/1‑36 (MQ=255) aTT‑A‑ACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCTCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCCCCAATTTTCTGGAAATCCTATGATGCCTTTc > 2:355706/1‑128 (MQ=255) aTT‑A‑ACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTcc < 1:986128/129‑1 (MQ=255) aTT‑A‑ACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTc < 1:355706/128‑1 (MQ=255) aTT‑A‑ACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTCAAATTTCTCAATAATGTCACCAATTCTCTGGAAATCCTATGATGCCTTTcc > 2:986128/1‑129 (MQ=255) | CTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑A‑A‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCC > NC_000913/2767306‑2767561 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |