| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,728,118 | (C)8→9 | coding (1254/1455 nt) | xylB ← | xylulokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,728,110 | 1 | . | C | 95.0% | 129.4 / ‑0.2 | 40 | coding (1262/1455 nt) | xylB | xylulokinase |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (17/21); total (18/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
AGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCA > NC_000913/3727980‑3728242 | aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑ccccc‑cc‑c < 1:570338/139‑1 (MQ=255) cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑ccccc‑cc‑ccg < 1:925323/139‑3 (MQ=255) cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑ccccc‑cc‑ccg > 2:251043/1‑137 (MQ=255) tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑ccccc‑cc‑ccgta > 2:232230/1‑126 (MQ=255) tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑ccccc‑cc‑ccgta < 1:232230/130‑5 (MQ=255) cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCgcg > 2:548801/1‑137 (MQ=255) cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCTATTTCTCTGGATTCGCCGCGATCTGCCCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAgctgct < 1:469352/139‑1 (MQ=255) ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGa < 2:88361/139‑1 (MQ=255) aGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCg > 2:720893/1‑139 (MQ=255) cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTCCCGTAGTCGAGCTGCTGGCCGCTGat > 2:834289/1‑139 (MQ=255) cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCCATTTCTCTGGATTCGCTGCGATCTGCGCCAGCCTTACTGCGCCCAGGCCTCGCCCCACAT‑CCCCC‑CCGCCGCCGGCGGTCGAGCTGCTGACCGCTCatacc > 1:665288/1‑137 (MQ=255) aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGTGCCCAGTGCTGGCCCCACAT‑cccc > 2:220995/1‑89 (MQ=255) aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGTGCCCAGTGCTGGCCCCACAT‑cccc < 1:220995/89‑1 (MQ=255) aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa > 1:853412/1‑139 (MQ=255) aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCACTGATATCCGCCAGCATCTGa > 2:770264/1‑139 (MQ=255) gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCC‑CC‑CGCACGGTAATCCAGCCCCTGACCGCCGACACCCGCCCGCATCTGACg > 1:892007/1‑139 (MQ=255) aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAc > 1:460427/1‑139 (MQ=255) caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTc < 2:861860/139‑1 (MQ=255) tCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa < 1:720893/139‑1 (MQ=255) aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcg < 1:888598/139‑1 (MQ=255) gCGAATTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCTAGTACTCACTACGcgccc < 2:665288/139‑1 (MQ=255) tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACTTCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt < 1:251043/139‑1 (MQ=255) tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc > 1:173304/1‑128 (MQ=255) tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc < 2:173304/128‑1 (MQ=255) cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑ccccc < 2:156708/52‑1 (MQ=255) cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑ccccc > 1:156708/1‑52 (MQ=255) gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTa > 1:830318/1‑139 (MQ=255) aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTTACCGCTGATATCCGCCAGCCTCTGACGCCAGTACTCCCTACGCGCCCCCCCCCCAATCAACGTAACACtt > 1:114456/1‑139 (MQ=255) cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg < 2:460427/139‑1 (MQ=255) aGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTa > 2:219481/1‑139 (MQ=255) ccTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTTCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCgg > 1:705359/1‑133 (MQ=255) ccTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc < 2:928291/87‑1 (MQ=255) ccTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc > 1:928291/1‑87 (MQ=255) ccTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCa < 2:705832/113‑1 (MQ=255) ccTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCa > 1:705832/1‑113 (MQ=255) ccTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCgg < 2:705359/133‑1 (MQ=255) gcgcCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg < 2:716379/123‑1 (MQ=255) gcgcCCAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCCGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg > 1:716379/1‑123 (MQ=255) cccAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc < 2:771083/95‑1 (MQ=255) cccAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa < 2:830318/139‑1 (MQ=255) cccAGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAATCACTACGcgccc > 1:771083/1‑95 (MQ=255) aGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg > 2:543078/1‑72 (MQ=255) aGTGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg < 1:543078/72‑1 (MQ=255) gTGCTGTCCCGACAC‑CCCCCGCC‑CGTACGGTAATCGTGCTGCTGACCGCTGATATCCGCCAGCATCTGAAGCCAGTACACACTGCGCGCCCCGCCCCCAATCAACGTAACACTTTGCGATTTAATACCGCAGGCATGCa < 1:593649/139‑1 (MQ=255) gTGCTGGCCCCACAT‑CCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc < 2:892007/139‑1 (MQ=255) tGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCTGACGCCAGTACTc > 1:591154/1‑79 (MQ=255) tGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTc < 2:591154/79‑1 (MQ=255) tGCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCACTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc < 1:770264/139‑1 (MQ=255) gCTGGCCCCACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACg < 1:827147/139‑1 (MQ=255) ccACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTACCACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa < 1:834289/139‑1 (MQ=255) ccACATCCCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa < 2:853412/139‑1 (MQ=255) | AGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCC‑CC‑CGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCA > NC_000913/3727980‑3728242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |