| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,970,032 | (G)6→7 | coding (1002/1047 nt) | wzzE → | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,970,026 | 1 | . | G | 98.0% | 172.9 / ‑3.3 | 51 | coding (996/1047 nt) | wzzE | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (25/25); total (25/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01 | |||||||||||
CCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGAC > NC_000913/3969903‑3970146 | ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCgg > 2:823350/1‑139 (MQ=255) tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCgggg < 2:195742/139‑1 (MQ=255) tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCgggg > 1:543576/1‑139 (MQ=255) gAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGc < 1:628892/139‑1 (MQ=255) ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTc > 2:979564/1‑139 (MQ=255) ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTc > 1:779028/1‑139 (MQ=255) ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑ggg‑g < 2:15399/118‑1 (MQ=255) ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑ggg‑g > 1:15399/1‑118 (MQ=255) cAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCAt > 2:912593/1‑139 (MQ=255) aCCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑ggg‑gggg > 1:689301/1‑114 (MQ=255) aCCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑ggg‑gggg < 2:689301/115‑2 (MQ=255) ggATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACccg > 1:204296/1‑139 (MQ=255) ggATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACccg > 1:470184/1‑139 (MQ=255) gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑ggg‑gg > 2:494797/1‑102 (MQ=255) gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑‑ggg‑gg < 1:494797/102‑1 (MQ=255) cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc > 2:504925/1‑139 (MQ=255) gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 1:504925/139‑1 (MQ=255) gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 2:3003/139‑1 (MQ=255) gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt < 2:779028/139‑1 (MQ=255) ttttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc < 1:912593/139‑1 (MQ=255) ttttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc < 1:491167/139‑1 (MQ=255) aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAAcc < 1:273075/118‑1 (MQ=255) aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAAcc > 2:273075/1‑118 (MQ=255) aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa > 2:781763/1‑130 (MQ=255) aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa < 1:781763/130‑1 (MQ=255) aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaacaac > 2:325684/1‑139 (MQ=255) tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca < 1:400601/139‑1 (MQ=255) tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca < 2:543576/139‑1 (MQ=255) tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca < 1:823350/139‑1 (MQ=255) tGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACtgctgc > 2:753192/1‑139 (MQ=255) ggAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCaa < 1:979564/139‑1 (MQ=255) gaagaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGgtg > 1:435788/1‑86 (MQ=255) gaagaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGgtg < 2:435788/86‑1 (MQ=255) ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCt < 2:470184/139‑1 (MQ=255) tAAAACGCGATAGCCCACGTCGTGCCTTCTTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca < 2:868593/110‑1 (MQ=255) tAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca > 1:868593/1‑110 (MQ=255) aaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt < 2:204296/139‑1 (MQ=255) cccACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACActgct > 1:351115/1‑102 (MQ=255) cccACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACActgct < 2:351115/102‑1 (MQ=255) cgtcgtGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACCGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAAt < 1:715294/139‑1 (MQ=255) tGCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg < 1:325684/139‑1 (MQ=255) gCCTTCCTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtgt > 2:436269/1‑139 (MQ=255) ccTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGGTAATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACGGCTGTGGTGATCGCAAAGGCGCTCGTCGCTTATTCGAAGAGAATCGATGGGAAAg > 1:687588/1‑139 (MQ=255) ccTGATGATTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAg > 1:267400/1‑139 (MQ=255) atgatTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTact > 2:273289/1‑139 (MQ=255) atgatTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTact > 2:168700/1‑139 (MQ=255) gatTATGCGGGGCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga < 1:436269/139‑1 (MQ=255) tttATGCGGTGCATTGTCG‑GGG‑GGGCTCATCGGGGCTGGTGTCGCCTTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGCCGCTCGCCTCTTATTCGAAGAGAATCGATGTGAAAGCactgac < 1:574924/138‑1 (MQ=255) tgcggggCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa < 1:868349/66‑1 (MQ=255) tgcggggCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa > 2:868349/4‑69 (MQ=255) tgcggggCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCg > 1:660029/4‑79 (MQ=255) tgcggggCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg < 1:20185/98‑1 (MQ=255) tgcggggCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg > 2:20185/4‑101 (MQ=255) tgcggggCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa > 1:54569/4‑120 (MQ=255) tgcggggCATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa < 2:54569/117‑1 (MQ=255) tgcggggCATTGTC‑‑GGGTGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCg < 2:660029/76‑1 (MQ=255) cATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg < 2:954794/126‑1 (MQ=255) cATTGTCG‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg > 1:954794/1‑126 (MQ=255) | CCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑‑GGG‑GGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGAC > NC_000913/3969903‑3970146 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |