| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 309,358 | C→T | *223* (TAG→TAA) | ecpB ← | ECP production pilus chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 309,358 | 0 | C | T | 100.0% | 127.3 / NA | 40 | *223* (TAG→TAA) | ecpB | ECP production pilus chaperone |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/19); total (21/19) | |||||||||||
TGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGC > NC_000913/309226‑309492 | tgctgcGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTc > 2:975995/1‑139 (MQ=255) cgcACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACggg > 2:491781/1‑139 (MQ=255) cTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATg < 2:795874/139‑1 (MQ=255) cgcTGGCATCGGGCTGAACGAACAAAAAGCCCAGGCCGAGGGC‑AACTGGGCCTTCCGGCCCGGGGAAAACCCGCCTAAAGGGAATGTTGTTAATCCTCTGCACAACCTAT‑TAATTCAAGGGAATGGAATTAATACCCTg > 1:35028/1‑139 (MQ=255) cgcTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCt > 1:938674/1‑139 (MQ=255) cTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGc > 1:147621/1‑139 (MQ=255) gaacgaacAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAAccc < 1:449270/139‑1 (MQ=255) gaacgaacAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGCGCAAccc < 2:938674/139‑1 (MQ=255) aacgaacAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCg > 1:493567/1‑139 (MQ=255) cgaacAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTc < 2:49738/139‑1 (MQ=255) aaGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAAc > 2:587488/1‑126 (MQ=255) aaGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAAc < 1:587488/126‑1 (MQ=255) cATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGc > 1:495311/1‑139 (MQ=255) ggCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCAc < 1:695471/139‑1 (MQ=255) agaACCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCg < 1:645963/139‑1 (MQ=255) aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCa > 1:120089/1‑75 (MQ=255) aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCa < 2:120089/75‑1 (MQ=255) aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGc > 1:530100/1‑139 (MQ=255) aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGc > 2:194070/1‑139 (MQ=255) aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGc > 2:34414/1‑139 (MQ=255) ccGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATg > 1:121861/1‑56 (MQ=255) ccGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATg < 2:121861/56‑1 (MQ=255) ccGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCAt < 2:493567/139‑1 (MQ=255) cgtAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAgg < 2:495311/139‑1 (MQ=255) aaaGGCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGgtag < 1:194070/139‑1 (MQ=255) gCATTGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAAt < 1:34414/139‑1 (MQ=255) ttGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATtct > 2:858960/1‑139 (MQ=255) tGTCGTTATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATtctc > 1:257904/1‑139 (MQ=255) ttATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTac < 1:858960/139‑1 (MQ=255) tATCCATCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTaca > 2:704618/1‑139 (MQ=255) tCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCttt > 2:143262/1‑64 (MQ=255) tCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCttt < 1:143262/64‑1 (MQ=255) tCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTaca > 2:891483/1‑133 (MQ=255) tCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTaca < 1:891483/133‑1 (MQ=255) tCTGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCtt > 2:844779/1‑139 (MQ=255) tGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATc < 2:334767/68‑1 (MQ=255) tGCAC‑AGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATc > 1:334767/1‑68 (MQ=255) cac‑aGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATc < 2:214567/109‑1 (MQ=255) cac‑aGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATc > 1:214567/1‑109 (MQ=255) c‑aGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGcc > 1:294818/1‑100 (MQ=255) c‑aGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGcc < 2:294818/100‑1 (MQ=255) ctatttatTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTggc > 2:411165/1‑139 (MQ=255) | TGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCAC‑AGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGC > NC_000913/309226‑309492 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |