| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,314,956 | +C | coding (1469/2673 nt) | rcsD → | phosphotransfer intermediate protein in two‑component regulatory system with RcsBC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,314,952 | 1 | . | C | 94.1% | 109.6 / 0.2 | 34 | coding (1465/2673 nt) | rcsD | phosphotransfer intermediate protein in two‑component regulatory system with RcsBC |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (16/16); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.89e-01 | |||||||||||
CAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACG‑C‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTT > NC_000913/2314822‑2315082 | cAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACG‑c‑ccccgga < 1:369015/139‑5 (MQ=255) aaCTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCa > 2:697599/1‑139 (MQ=255) aatCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTCATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACGGTCCCGGGCGGAGAGAACGGCTAAAATCAACG‑CGCCCGGAAAGCaa > 2:700106/3‑139 (MQ=255) tCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAaac < 1:39359/139‑1 (MQ=255) gCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCa < 1:20989/139‑1 (MQ=255) aCCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGc > 2:1020690/1‑139 (MQ=255) ccAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCg < 1:697599/139‑1 (MQ=255) agGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTgg > 2:33356/1‑139 (MQ=255) gCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTtga > 1:420999/1‑139 (MQ=255) cGGATGATCTTTATGCAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTtgat > 2:578495/1‑139 (MQ=255) cGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTtgat < 1:77254/139‑1 (MQ=255) ggATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTtgatg < 1:500699/139‑1 (MQ=255) tgatCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGaaa < 2:857084/139‑1 (MQ=255) aaaCATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGaa > 2:152599/1‑139 (MQ=255) aaCATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAAc > 2:947647/1‑139 (MQ=255) tGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTtg < 2:961683/114‑1 (MQ=255) tGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTtg > 1:961683/1‑114 (MQ=255) gCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGt < 1:745309/110‑1 (MQ=255) gCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGt > 2:745309/1‑110 (MQ=255) gCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACG‑c‑cccc < 1:1029572/62‑2 (MQ=255) gCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACG‑c‑cccc > 2:1029572/1‑61 (MQ=255) cgcTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGgatg > 2:1100771/1‑139 (MQ=255) gcTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGgatga < 1:1020690/139‑1 (MQ=255) gAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAg < 1:152599/139‑1 (MQ=255) aCCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGaa > 1:507919/1‑139 (MQ=255) cacaGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTg > 1:1036794/1‑139 (MQ=255) cacaGTCCCTGGCGGAGAGCGCGGCTAAACTCAACG‑CTCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATAc > 2:320130/1‑100 (MQ=255) cacaGTCCCTGGCGGAGAGCGCGGCTAAACTCAACG‑CTCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATAc < 1:320130/100‑1 (MQ=255) tCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTtga > 2:907639/1‑87 (MQ=255) tCCCTGGCGGAGAGCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTtga < 1:907639/87‑1 (MQ=255) agagCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTgaga > 2:406128/1‑127 (MQ=255) agagCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTgaga < 1:406128/127‑1 (MQ=255) agagCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTtct < 1:1100771/139‑1 (MQ=255) agagCGCGGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTtct > 2:929411/1‑139 (MQ=255) gcgGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTg < 2:420999/139‑1 (MQ=255) cgGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCg < 1:131334/39‑1 (MQ=255) cgGCTAAACTCAACGCC‑CCCGGAAAGCAAACAACTGGCg > 2:131334/1‑39 (MQ=255) aCTCAACGCC‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGAttt < 1:947647/139‑1 (MQ=255) | CAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACG‑C‑CCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTGCAGGATTT > NC_000913/2314822‑2315082 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |