| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,484,897 | (C)6→7 | coding (524/3594 nt) | evgS → | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,484,891 | 1 | . | C | 92.3% | 121.3 / 3.3 | 41 | coding (518/3594 nt) | evgS | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base C (18/18); total (20/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTA > NC_000913/2484753‑2485019 | cTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa < 2:477102/139‑1 (MQ=255)cTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa < 1:456146/139‑1 (MQ=255)cTTCGACGCCTCTTAATAATGACATTGCTTCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCCTCACGACTCAATGCGACCGCTTACCTCCCCAAAACCAGTAAATATTGCGCGGGTAGGAAATTa < 2:49825/139‑1 (MQ=255) ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c < 1:47233/139‑1 (MQ=255) cgccgcCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑ccc > 2:993905/1‑139 (MQ=255) ccgccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑ccc‑c‑c < 1:469545/139‑1 (MQ=255) cgccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑ccc‑c‑cc > 1:374733/1‑139 (MQ=255) gccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑ccc‑c‑ccc > 1:327701/1‑138 (MQ=255) tctTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATTTTGCTCGGGTAGCAAATTA‑ccc‑c‑cccaga < 1:743209/139‑5 (MQ=255) tctTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑ccc‑c‑cccaga < 1:371626/139‑5 (MQ=255) gACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑ccc‑c‑cccaga < 1:937160/129‑5 (MQ=255) gACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑ccc‑c‑cccaga > 2:937160/1‑125 (MQ=255) cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAACACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATtca > 1:210645/1‑139 (MQ=255) cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATtca < 1:993905/139‑1 (MQ=255) aaTTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGATTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCC‑CTCCAGACGAGGTAATtca > 1:683188/2‑139 (MQ=255) ctgcAACCAAACCATTGATAATTAACTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCatcaat < 1:620621/139‑1 (MQ=255) aTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGc < 2:1003313/139‑1 (MQ=255) aTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGc < 1:211610/139‑1 (MQ=255) tGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCatcaa > 1:1048952/1‑123 (MQ=255) tGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCatcaa < 2:1048952/123‑1 (MQ=255) tGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑ccc‑c‑ccc > 1:871874/1‑103 (MQ=255) tGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑ccc‑c‑ccc < 2:871874/104‑2 (MQ=255) cGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa < 2:683188/139‑1 (MQ=255) cgcTGGTAACCACCCTTCGCGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaa > 2:616216/1‑121 (MQ=255) cgcTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaa < 1:616216/121‑1 (MQ=255) gTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTta > 2:491873/1‑139 (MQ=255) cccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGc < 2:446439/139‑1 (MQ=255) ccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaaca < 1:127757/110‑1 (MQ=255) ccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaaca > 2:127757/1‑110 (MQ=255) ccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑ccc‑c‑cccaga > 2:391075/1‑72 (MQ=255) ccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑ccc‑c‑cccaga < 1:391075/76‑5 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTATCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt > 1:167694/1‑139 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGc < 1:860536/104‑1 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGc > 2:70400/1‑104 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGc > 2:860536/1‑104 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGc < 1:70400/104‑1 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtc > 1:364300/1‑114 (MQ=255) ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtc < 2:364300/114‑1 (MQ=255) cACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATAAGGCATTa > 2:600925/1‑139 (MQ=255) tCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATcc > 2:481757/1‑139 (MQ=255) aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGt > 2:1059702/1‑139 (MQ=255) tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct < 2:994631/139‑1 (MQ=255) tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct > 1:393877/1‑139 (MQ=255) tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct > 1:260907/1‑139 (MQ=255) cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATtca > 2:127345/1‑66 (MQ=255) cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATtca < 1:127345/66‑1 (MQ=255) ttACCTCACCAAAACCATTAAAAATTGCCCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc < 2:372801/139‑1 (MQ=255) aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa < 2:311348/93‑1 (MQ=255) aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa > 1:311348/1‑93 (MQ=255) tAAATATTGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTg > 1:828862/1‑139 (MQ=255) atatatTGCTCGGGTAGCAAATTA‑CCCTC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTgg < 2:393877/137‑1 (MQ=255) ttGCTCGGGTAGCAAATTATCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagta < 2:167694/139‑1 (MQ=255) ttGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagta < 2:828862/139‑1 (MQ=255) tGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc > 1:779964/1‑114 (MQ=255) tGCTCGGGTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc < 2:779964/114‑1 (MQ=255) gTAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCAtt > 2:678233/1‑139 (MQ=255) tAGCAAATTACCCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTa < 1:481757/139‑1 (MQ=255) | CTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCC‑C‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTA > NC_000913/2484753‑2485019 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |