| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,999,680 | (G)7→8 | coding (212/756 nt) | ygeR ← | LysM domain‑containing M23 family putative peptidase, septation lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,999,673 | 1 | . | G | 95.9% | 164.7 / ‑0.5 | 49 | coding (219/756 nt) | ygeR | LysM domain‑containing M23 family putative peptidase, septation lipoprotein |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (21/26); total (21/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AA‑‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTC > NC_000913/2999548‑2999788 | ttCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATg > 1:983946/1‑139 (MQ=255) gCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGt < 2:85140/139‑1 (MQ=255) cTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAGGGGGGGGGAAATGCCGTTCAGt < 1:655252/139‑1 (MQ=255) cTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTc > 1:819383/1‑139 (MQ=255) cTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTc > 1:811953/1‑139 (MQ=255) atgaCGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTcgcg > 2:604629/1‑139 (MQ=255) tgtAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTc > 2:174451/1‑139 (MQ=255) aaCCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCttt > 1:43021/1‑139 (MQ=255) ccGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTa > 1:1005022/1‑139 (MQ=255) gATGCGGTTTTGGTCGTTGATTTGGCGGTTGCTTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACg < 2:390776/139‑1 (MQ=255) aTGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGc > 2:377441/1‑139 (MQ=255) gCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTg > 1:317734/1‑139 (MQ=255) cGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTgg < 1:551326/139‑1 (MQ=255) ggTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGt > 1:918598/1‑139 (MQ=255) ttGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AA‑‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCg < 2:183415/139‑1 (MQ=255) gTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCgtg > 1:427125/1‑139 (MQ=255) cGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAA‑‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCgtggt < 2:817938/139‑1 (MQ=255) tGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTgcg < 1:558724/139‑1 (MQ=255) tGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTgcg < 1:604629/139‑1 (MQ=255) aTTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGcgcg < 1:174451/139‑1 (MQ=255) aTTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGcgcg < 2:1005022/139‑1 (MQ=255) ttGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGat < 2:333335/139‑1 (MQ=255) aTTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGAtata < 1:377441/139‑1 (MQ=255) aaTACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATcccc > 2:684925/1‑139 (MQ=255) tGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGcgcg > 1:92279/1‑112 (MQ=255) tGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGcgcg < 2:92279/112‑1 (MQ=255) cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGaaa > 2:697139/1‑113 (MQ=255) cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGaaa < 1:697139/113‑1 (MQ=255) cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAt < 1:668766/128‑1 (MQ=255) cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTc > 2:595197/1‑139 (MQ=255) cTACTTTTCGCCCCACCCAGTGTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTAGGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAt > 2:668766/1‑128 (MQ=255) aCTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCAc < 1:595197/139‑1 (MQ=255) cGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTcgcg < 2:472946/69‑1 (MQ=255) cGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTcgcg > 1:472946/1‑69 (MQ=255) cGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTa > 1:1022071/1‑139 (MQ=255) ccacccaGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACg < 2:317734/139‑1 (MQ=255) cacccaGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACgg < 2:427125/139‑1 (MQ=255) ccaGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGc < 2:43021/139‑1 (MQ=255) ccaGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGc < 2:819383/139‑1 (MQ=255) ccaGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGc < 2:983946/139‑1 (MQ=255) ttttAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGa < 1:684925/139‑1 (MQ=255) tAGTTTCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCTAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa < 2:212528/139‑1 (MQ=255) tCTGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTc < 2:1022071/139‑1 (MQ=255) tGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCgtggtg < 1:847623/76‑1 (MQ=255) tGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCgtggtg > 2:847623/1‑76 (MQ=255) tGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGc > 2:198212/1‑125 (MQ=255) tGACCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGc < 1:198212/125‑1 (MQ=255) aCCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCgcgc > 2:1076946/1‑46 (MQ=255) aCCAACTTCAATGGTGT‑AAG‑GGGGGGGAAATGCCGTTCAGTCgcgc < 1:1076946/46‑1 (MQ=255) | TTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGT‑AA‑‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTC > NC_000913/2999548‑2999788 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |