Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	571,444	G→A	*184* (TAG→TAA)	ybcL →	inactive polymorphonuclear leukocyte migration suppressor, DLP12 prophage, UPF0098 family secreted protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	571,444	0	G	A	100.0%	141.5 / NA	44	*184* (TAG→TAA)	ybcL	inactive polymorphonuclear leukocyte migration suppressor, DLP12 prophage, UPF0098 family secreted protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/22);  total (22/22)

AGTTTAAAGTATGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAGGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGGAAAGAAAGTTAT  >  NC_000913/571311‑571578                                                                                                                                       |                                                                                                                                       aGTTTAAAGTATGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTga                                                                                                                                   <  1:283357/139‑1 (MQ=255)            tGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTgg                                                                                                                        >  1:98297/1‑139 (MQ=255)             gggCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGc                                                                                                                       >  2:722479/1‑139 (MQ=255)             gggCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGc                                                                                                                       >  1:305861/1‑139 (MQ=255)                          tGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACt                                                                                                          <  1:635029/139‑1 (MQ=255)                                    ccTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAg                                                                                                 >  1:922129/1‑139 (MQ=255)                                               tAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGAt                                                                                               >  2:629888/1‑129 (MQ=255)                                               tAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGAt                                                                                               <  1:629888/129‑1 (MQ=255)                                                   tCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGa                                                                                 <  2:978608/139‑1 (MQ=255)                                                   tCCAGCGGAGCATTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGGAATACGGa                                                                                 <  1:722479/139‑1 (MQ=255)                                                       gCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCa                                                                             <  2:693739/139‑1 (MQ=255)                                                             cGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTgaga                                                                                                                                 >  2:955050/1‑81 (MQ=255)                                                             cGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTgaga                                                                                                                                 <  1:955050/81‑1 (MQ=255)                                                             cGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAAca                                                                       >  1:603112/1‑139 (MQ=255)                                                                       ttATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGAtt                                                             >  1:131246/1‑139 (MQ=255)                                                                         atatGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGa                                                                                 <  1:228872/117‑1 (MQ=255)                                                                         atatGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGa                                                                                 >  2:228872/1‑117 (MQ=255)                                                                         atatGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCa                                                           <  2:707178/139‑1 (MQ=255)                                                                          tatGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCAt                                                          >  1:888700/1‑139 (MQ=255)                                                                          tatGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCAt                                                          <  1:942399/139‑1 (MQ=255)                                                                                 aaTGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCa                                                                                                                      >  1:32516/1‑72 (MQ=255)                                                                                 aaTGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCa                                                                                                                      <  2:32516/72‑1 (MQ=255)                                                                                   tGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAg                                                 <  1:160483/139‑1 (MQ=255)                                                                                       aataaAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACt                                                                                                          <  1:954354/78‑1 (MQ=255)                                                                                       aataaAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACt                                                                                                          >  2:954354/1‑78 (MQ=255)                                                                                        ataaAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGTCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCctct                                            >  2:680833/1‑139 (MQ=255)                                                                                        ataaAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACt                                                                                                          <  1:917399/77‑1 (MQ=255)                                                                                        ataaAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACt                                                                                                          >  2:917399/1‑77 (MQ=255)                                                                                         taaAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGt                                                                                                                                     <  1:737819/49‑1 (MQ=255)                                                                                         taaAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGt                                                                                                                                     >  2:737819/1‑49 (MQ=255)                                                                                             aTCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAagg                                                                                                                                      <  1:64381/44‑1 (MQ=255)                                                                                             aTCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGc                                       <  2:131246/139‑1 (MQ=255)                                                                                                  aaCCGCTGAGATAACACCAGTTTATGAGAT‑ACAGTAAGGTGAGAGTATGCTGGCa                                                                                                                      >  1:477767/1‑55 (MQ=39)                                                                                                  aaCCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCa                                                                                                                      <  2:477767/55‑1 (MQ=255)                                                                                                  aaCCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCgaag                                                                                          >  2:780483/1‑83 (MQ=255)                                                                                                  aaCCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCgaag                                                                                          <  1:780483/83‑1 (MQ=255)                                                                                                                    aGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGAc                                                                                                           >  2:331767/1‑48 (MQ=255)                                                                                                                    aGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGAc                                                                                                           <  1:331767/48‑1 (MQ=255)                                                                                                                    aGTTTATGAGAT‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAg                >  2:1001120/1‑139 (MQ=255)                                                                                                                               t‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTAt                       <  2:106664/121‑1 (MQ=255)                                                                                                                               t‑AAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTAt                       >  1:106664/1‑121 (MQ=255)                                                                                                                                 aaaGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACt                                                                                                          >  2:505408/1‑37 (MQ=255)                                                                                                                                 aaaGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACt                                                                                                          <  1:505408/37‑1 (MQ=255)                                                                                                                                 aaaGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGGAAAGAAAGtt    >  1:525177/1‑139 (MQ=255)                                                                                                                                   aGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGGAAAGAAAGTTAt  <  2:922129/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       AGTTTAAAGTATGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGAT‑AAAGTAGGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGGAAAGAAAGTTAT  >  NC_000913/571311‑571578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑