Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,380,807	G→A	*220* (TAG→TAA)	ycjU →	beta‑phosphoglucomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,380,807	0	G	A	100.0%	104.8 / NA	33	*220* (TAG→TAA)	ycjU	beta‑phosphoglucomutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/18);  total (15/18)

ACGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTC‑AAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATC  >  NC_000913/1380680‑1380919                                                                                                                                |                                                                                                                  aCGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCa                                                                                                        >  1:395162/1‑139 (MQ=255)    ccATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACa                                                                                                     <  1:297816/139‑1 (MQ=255)              cAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCt                                                                                           <  2:289594/139‑1 (MQ=255)                       gcgcTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTaca                                                                                  <  1:837834/139‑1 (MQ=255)                              gTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATAtt                                                                           >  1:847429/1‑139 (MQ=255)                                      cGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTC‑AAAAAAt                                                                  <  1:972424/139‑1 (MQ=255)                                         cgcgGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTa                                                                                    >  2:90891/1‑119 (MQ=255)                                         cgcgGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTa                                                                                    <  1:90891/119‑1 (MQ=255)                                         cgcgGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTC‑AAAAAATCTa                                                               <  1:954407/139‑1 (MQ=255)                                                        ggCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTC‑AAAAAATCTACGATAACCAGGTGCa                                                >  1:687239/1‑139 (MQ=255)                                                        ggCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTC‑AAAAAATCTACGATAACCAGGTGCa                                                <  2:847429/139‑1 (MQ=255)                                                        ggCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTC‑AAAAAATCTACGATAACCAGGTGCa                                                >  1:96062/1‑139 (MQ=255)                                                        ggCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTC‑AAAAAATCTACGATAACCAGGTGCa                                                >  2:253979/1‑139 (MQ=255)                                                          cgcAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAAATCTACGATAACCAGGTGCAt                                               >  1:790626/1‑139 (MQ=255)                                                          cgcAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTC‑AAAAAATCTACGATAACCAGGTGCAtg                                              <  2:687239/139‑1 (MQ=255)                                                           gcAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTCAAAAAAATCTACGATAACCAGGTGCAtg                                              <  2:790626/139‑1 (MQ=255)                                                                 aCTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTC‑AAAAAATCTACGATAACCAGGTGCATGTGGTGaa                                       <  2:395162/139‑1 (MQ=255)                                                                         cTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTC‑AAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCa                               >  2:30719/1‑139 (MQ=255)                                                                          ttCAACGGAATCACTCACCTGGCCTCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTC‑AAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCaa                              >  1:39284/1‑139 (MQ=255)                                                                          ttCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCt                                                                                                <  1:862204/74‑1 (MQ=255)                                                                          ttCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCt                                                                                                >  2:862204/1‑74 (MQ=255)                                                                                   aTCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTC‑AAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAAt                     <  2:96062/139‑1 (MQ=255)                                                                                        tcacCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTC‑AAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCg                <  1:253979/139‑1 (MQ=255)                                                                                              ggCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTacaaca                                                                               >  2:1043675/1‑71 (MQ=255)                                                                                              ggCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTacaaca                                                                               <  1:1043675/71‑1 (MQ=255)                                                                                               gCCGCGGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTC‑AAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAaga         >  2:793432/1‑139 (MQ=255)                                                                                                 cgcgGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTC‑AAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAAt                     >  2:1043546/1‑125 (MQ=255)                                                                                                 cgcgGTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTC‑AAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAAt                     <  1:1043546/125‑1 (MQ=255)                                                                                                    ggTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTC‑AAAAAATCTACGATAACCAGGTGCAt                                               >  2:1095335/1‑96 (MQ=255)                                                                                                    ggTTATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTC‑AAAAAATCTACGATAACCAGGTGCAt                                               <  1:1095335/96‑1 (MQ=255)                                                                                                      ttATCGGCCTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTC‑AAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATc  <  2:558771/139‑1 (MQ=255)                                                                                                              cTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCt                                                                                                >  1:85003/1‑38 (MQ=255)                                                                                                              cTTCTGGCAAAACGTATAACAAAGGAATCAACATGGCt                                                                                                <  2:85003/38‑1 (MQ=255)                                                                                                                                |                                                                                                                  ACGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCACTCACCTGGCCGCGGTTATCGGCCTTCTGGCAAAACGTATAGCAAAGGAATCAACATGGCTCAGCTTTCGTTACAACATATTC‑AAAAAATCTACGATAACCAGGTGCATGTGGTGAAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATC  >  NC_000913/1380680‑1380919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑