| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,815,811 | C→T | intergenic (‑43/+23) | pyrE ← / ← rph | orotate phosphoribosyltransferase/ribonuclease PH (defective),enzyme, Degradation of RNA, RNase PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,815,811 | 0 | C | T | 97.1% | 106.9 / ‑3.4 | 35 | intergenic (‑43/+23) | pyrE/rph | orotate phosphoribosyltransferase/ribonuclease PH (defective),enzyme, Degradation of RNA, RNase PH |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (17/17); minor base . (0/1); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01 | |||||||||||
GGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCC > NC_000913/3815675‑3815941 | ggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGTGa > 1:614484/1‑139 (MQ=255) gcgcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGTGACtcatca < 2:18580/139‑1 (MQ=255) aaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGG‑GACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgcc < 1:1106071/139‑1 (MQ=255) aCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgcc > 1:970793/1‑139 (MQ=255) gcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑‑AAAAAAAGGTGACTCATCAGt > 1:292013/1‑99 (MQ=255) gcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑‑AAAAAAAGGTGACTCATCAGt < 2:292013/99‑1 (MQ=255) gcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGga < 2:614484/139‑1 (MQ=255) aTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTcc > 2:1107265/1‑139 (MQ=255) ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCggg > 1:638628/1‑139 (MQ=255) gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGc < 1:760873/139‑1 (MQ=255) tGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCaga < 2:371816/139‑1 (MQ=255) atatGGTTTCATGCCTTCGCTCCTCATCTCACTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGc < 1:752063/139‑1 (MQ=255) ggTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaac < 2:638628/139‑1 (MQ=255) ttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAg < 2:517627/139‑1 (MQ=255) aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGact > 1:603931/1‑137 (MQ=255) aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATg > 2:856529/1‑139 (MQ=255) aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATg < 2:952371/139‑1 (MQ=255) aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATg > 1:952371/1‑139 (MQ=255) ttCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAg > 2:307517/1‑139 (MQ=255) tcctcATCTTACTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCaga > 1:181081/1‑117 (MQ=255) tcctcATCTTACTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCaga < 2:181081/117‑1 (MQ=255) tcctcATCTTACTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt < 1:686946/139‑1 (MQ=255) aTCTTACTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTg < 2:486541/76‑1 (MQ=255) aTCTTACTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTg > 1:486541/1‑76 (MQ=255) aCTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCg < 2:498451/73‑1 (MQ=255) aCTTTTCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCg > 1:498451/1‑73 (MQ=255) ttCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGcc < 2:429901/100‑1 (MQ=255) ttCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGcc > 1:429901/1‑100 (MQ=255) tCTACAGACAAAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGc > 2:305946/1‑139 (MQ=255) tCTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCt > 1:972649/1‑139 (MQ=255) cTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCt > 1:718188/1‑92 (MQ=255) cTACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCt < 2:718188/92‑1 (MQ=255) tACAGAC‑AAAAAAAAGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCg > 2:809469/1‑139 (MQ=255) aaaaaaaaGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgat > 1:806969/1‑73 (MQ=255) aaaaaaaaGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGgat < 2:806969/73‑1 (MQ=255) aaaaaaGGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc < 1:307517/139‑1 (MQ=255) | GGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCC > NC_000913/3815675‑3815941 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |