Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	344,991	G→A	*272* (TAG→TAA)	yahL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	344,991	0	G	A	100.0%	307.4 / NA	93	*272* (TAG→TAA)	yahL	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (52/41);  total (52/41)

ATTGCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGG  >  NC_000913/344856‑345125                                                                                                                                         |                                                                                                                                       aTTGCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTc                                                                                                                                     >  2:95787/1‑139 (MQ=255)    gCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGa                                                                                                                                  >  1:337219/1‑139 (MQ=255)          tGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGt                                                                                                                            >  2:650208/1‑139 (MQ=255)           gTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGtt                                                                                                                           <  2:337219/139‑1 (MQ=255)            ttCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTc                                                                                                                          >  2:1034262/1‑139 (MQ=255)                 gACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCt                                                                                                                     >  1:276442/1‑139 (MQ=255)                    gTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                                  >  2:333282/1‑139 (MQ=255)                             tACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAt                                                                                                         >  1:933794/1‑139 (MQ=255)                              aCAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                                        <  2:98879/139‑1 (MQ=255)                                  agaTGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAc                                                                                                    >  2:290370/1‑139 (MQ=255)                                   gatgatGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAcc                                                                                                   <  1:650208/139‑1 (MQ=255)                                   gatgatGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAcc                                                                                                   >  2:1026248/1‑139 (MQ=255)                                        tgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTgaga                                                                                              <  1:95787/139‑1 (MQ=255)                                          cgcgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGt                                                                                            <  1:573067/139‑1 (MQ=255)                                           gcgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                           <  1:290370/139‑1 (MQ=255)                                              aTTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAGAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAAt                                                                                        <  1:1081046/139‑1 (MQ=255)                                                   atgaatgGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCaa                                                                                   <  1:1034262/139‑1 (MQ=255)                                                         ggATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                                  <  2:304777/102‑1 (MQ=255)                                                         ggATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                                  >  1:304777/1‑102 (MQ=255)                                                            tatCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTaa                                                                          <  2:337893/139‑1 (MQ=255)                                                                   tAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                   >  1:735608/1‑139 (MQ=255)                                                                    agagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCt                                                                                                  <  2:966664/107‑1 (MQ=255)                                                                    agagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCt                                                                                                  >  1:966664/1‑107 (MQ=255)                                                                     gagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCaa                                                                                   >  1:422075/1‑121 (MQ=255)                                                                     gagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCaa                                                                                   >  1:728921/1‑121 (MQ=255)                                                                     gagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCaa                                                                                   <  2:728921/121‑1 (MQ=255)                                                                     gagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCaa                                                                                   <  2:422075/121‑1 (MQ=255)                                                                      agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgttgt                                                                                                            >  2:1031430/1‑95 (MQ=255)                                                                      agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgttgt                                                                                                            <  1:1031430/95‑1 (MQ=255)                                                                       gTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGc                                                                                     >  1:442963/1‑117 (MQ=255)                                                                       gTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGc                                                                                     <  2:442963/117‑1 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGaa                                                                                                                                 >  1:365822/1‑72 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGaa                                                                                                                                 <  2:365822/72‑1 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgttgt                                                                                                            <  1:891627/93‑1 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgttgt                                                                                                            >  2:891627/1‑93 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                                        >  2:104661/1‑97 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                                        <  1:104661/97‑1 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGTATCGCAATGCAGGTAATCATTTCatctg                                                              >  1:666196/1‑139 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                   >  1:847732/1‑134 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                   <  2:847732/134‑1 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctg                                                              >  1:457796/1‑139 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                 <  2:545520/136‑1 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                 >  1:545520/1‑136 (MQ=255)                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTACACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctg                                                              >  2:680583/1‑139 (MQ=255)                                                                         gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATTCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                                  >  2:376370/1‑87 (MQ=255)                                                                         gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATTCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                                  <  1:376370/87‑1 (MQ=255)                                                                          cAGGACTTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAATGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgta                                                            <  1:691462/139‑1 (MQ=255)                                                                          cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                   <  2:983824/132‑1 (MQ=255)                                                                          cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                   >  1:983824/1‑132 (MQ=255)                                                                          cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgta                                                            >  1:676187/1‑139 (MQ=255)                                                                          cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                 <  1:439037/134‑1 (MQ=255)                                                                          cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                 >  2:439037/1‑134 (MQ=255)                                                                           aGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtat                                                           <  2:933794/139‑1 (MQ=255)                                                                                  aGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAgg                                                                                                                              <  2:73879/65‑1 (MQ=255)                                                                                  aGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAgg                                                                                                                              >  1:73879/1‑65 (MQ=255)                                                                                  aGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtatatctgta                                                    >  1:341808/1‑139 (MQ=255)                                                                                       aCAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCa                                               >  1:961199/1‑139 (MQ=255)                                                                                        cAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                           <  2:1046344/94‑1 (MQ=255)                                                                                        cAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                           >  1:1046344/1‑94 (MQ=255)                                                                                             attatCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgtt                                                                                                              <  1:1011252/70‑1 (MQ=255)                                                                                             attatCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAgtt                                                                                                              >  2:1011252/1‑70 (MQ=255)                                                                                                    tGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAt                                  <  2:961199/139‑1 (MQ=255)                                                                                                      tAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                                  <  1:1087559/57‑1 (MQ=255)                                                                                                      tAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                                  >  2:1087559/1‑57 (MQ=255)                                                                                                      tAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTa                                                                                                          >  1:231243/1‑65 (MQ=255)                                                                                                      tAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTa                                                                                                          <  2:231243/65‑1 (MQ=255)                                                                                                      tAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgta                                                            <  1:606307/111‑1 (MQ=255)                                                                                                      tAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgta                                                            >  2:606307/1‑111 (MQ=255)                                                                                                      tAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAtct                                >  1:533828/1‑139 (MQ=255)                                                                                                      tAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAtct                                >  1:16073/1‑139 (MQ=255)                                                                                                      tAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAtct                                >  2:432555/1‑139 (MQ=255)                                                                                                           aaGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                                  >  2:915838/1‑52 (MQ=255)                                                                                                           aaGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCa                                                                                                                  <  1:915838/52‑1 (MQ=255)                                                                                                           aaGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                           >  2:117122/1‑75 (MQ=255)                                                                                                           aaGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                           <  1:117122/75‑1 (MQ=255)                                                                                                           aaGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCtttt                           >  2:613224/1‑139 (MQ=255)                                                                                                           aaGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCtttt                           >  2:678993/1‑139 (MQ=255)                                                                                                           aaGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCtttt                           >  2:432624/1‑139 (MQ=255)                                                                                                           aaGCACGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCtttt                           >  2:27939/1‑139 (MQ=255)                                                                                                                cGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTgaga                                                                                              >  2:235644/1‑67 (MQ=255)                                                                                                                cGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTgaga                                                                                              <  1:235644/67‑1 (MQ=255)                                                                                                                cGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                 >  1:432699/1‑96 (MQ=255)                                                                                                                cGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                 <  2:432699/96‑1 (MQ=255)                                                                                                                cGTTAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGc                      >  2:857559/1‑139 (MQ=255)                                                                                                                  ttAAAA‑TCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCg                    <  1:613224/139‑1 (MQ=255)                                                                                                                              tACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTttgttg         <  1:432624/139‑1 (MQ=255)                                                                                                                                  ctctTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAtc                                 <  1:467408/111‑1 (MQ=255)                                                                                                                                  ctctTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAtc                                 >  2:467408/1‑111 (MQ=255)                                                                                                                                  ctctTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGAt     <  2:16073/139‑1 (MQ=255)                                                                                                                                   tctTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATc    >  1:78323/1‑139 (MQ=255)                                                                                                                                     tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCt                                                                                                  >  2:741404/1‑43 (MQ=255)                                                                                                                                     tttAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCgg  >  2:895886/1‑139 (MQ=255)                                                                                                                                     tttAATTCAGAAAGGGTTCCACCAGCAAGTTGTTATTCCACCt                                                                                                  <  1:741404/43‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       ATTGCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAA‑TCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGG  >  NC_000913/344856‑345125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑