Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,515,284	C→T	G465D (GGC→GAC)	fecA ←	TonB‑dependent outer membrane ferric citrate transporter and signal transducer, ferric citrate extracelluar receptor, FecR‑interacting protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,515,284	0	C	T	100.0%	174.1 / NA	55	G465D (GGC→GAC)	fecA	TonB‑dependent outer membrane ferric citrate transporter and signal transducer, ferric citrate extracelluar receptor, FecR‑interacting protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/31);  total (24/31)

TTGTTCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTC  >  NC_000913/4515149‑4515417                                                                                                                                         |                                                                                                                                      ttgttCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGGCTTGAGTCg                                                                                                                                     >  2:521985/1‑139 (MQ=255)   gttCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGAc                                                                                                                                  >  1:580325/1‑139 (MQ=255)         gTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAAc                                                                                                                            >  2:821341/1‑139 (MQ=255)              aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGccc                                                                                                                       >  2:880359/1‑139 (MQ=255)              aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGccc                                                                                                                       >  1:271243/1‑139 (MQ=255)              aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGccc                                                                                                                       >  1:542779/1‑139 (MQ=255)              aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGccc                                                                                                                       <  2:271243/139‑1 (MQ=255)                       gTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggt                                                                                                              <  2:1045225/139‑1 (MQ=255)                       gTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggt                                                                                                              <  2:391875/139‑1 (MQ=255)                        ttCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtg                                                                                                             >  2:686901/1‑139 (MQ=255)                         tCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                            <  2:542779/139‑1 (MQ=255)                          cGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGc                                                                                                           <  1:833910/139‑1 (MQ=255)                          cGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGc                                                                                                           <  1:546622/139‑1 (MQ=255)                           gAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGc                                                                                                           <  1:521985/139‑1 (MQ=255)                           gAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCg                                                                                                          <  1:686901/139‑1 (MQ=255)                            aaaCGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCgg                                                                                                         <  2:970282/139‑1 (MQ=255)                            aaaCGCATACCCGACGGGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGACACCAGGCGTGGGCCTCGGTGCCGGCACGCCTATCGCGGTCGTAA‑CGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCgg                                                                                                         <  2:733029/139‑1 (MQ=255)                                gCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTgta                                                                                                     <  2:580325/139‑1 (MQ=255)                                  aTACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTgtagt                                                                                                   >  2:557912/1‑139 (MQ=255)                                  aTACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTgtagt                                                                                                   >  1:106789/1‑139 (MQ=255)                                    aCCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTaa                                                                                                 <  2:244359/139‑1 (MQ=255)                                              aTGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGa                                                                                                                                   <  1:576119/95‑1 (MQ=255)                                              aTGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGa                                                                                                                                   >  2:576119/1‑95 (MQ=255)                                                          ccGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGattcat                                                                           <  2:203711/139‑1 (MQ=255)                                                           cGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGattcatt                                                                          <  1:880359/139‑1 (MQ=255)                                                            gATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCgctgct                                                                                                                 >  2:666128/1‑99 (MQ=255)                                                            gATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCgctgct                                                                                                                 <  1:666128/99‑1 (MQ=255)                                                                      ttGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGAt                                                               <  1:875922/139‑1 (MQ=255)                                                                        gTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAg                                                             <  1:113572/139‑1 (MQ=255)                                                                            tcaaGATAGCAGGCGTGAGACTCGCGGCCGGAACGCGTATCGCGGTCGTAA‑CGTCATATGTAGGACGGCAACTGCCCGCTGCTGTTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCGATCAAATAGCGAGAgccaa                                                         <  2:987268/136‑3 (MQ=255)                                                                             ccAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGcc                                                                                                                        <  2:814535/75‑1 (MQ=255)                                                                             ccAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGcc                                                                                                                        >  1:814535/1‑75 (MQ=255)                                                                              cAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGa                                                                                >  1:507427/1‑114 (MQ=255)                                                                              cAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGa                                                                                <  2:507427/114‑1 (MQ=255)                                                                                 aTACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAgcccacgccc                                                    >  1:598980/1‑139 (MQ=255)                                                                                       ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGa                                                                                >  1:694337/1‑105 (MQ=255)                                                                                       ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGa                                                                                <  2:694337/105‑1 (MQ=255)                                                                                        gCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGt                                             <  2:106789/139‑1 (MQ=255)                                                                                          gTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTgc                                           <  1:557912/139‑1 (MQ=255)                                                                                           tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGatt                                                                              <  1:1040532/103‑1 (MQ=255)                                                                                           tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGatt                                                                              >  2:1040532/1‑103 (MQ=255)                                                                                           tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACt                                                 <  2:1090700/132‑1 (MQ=255)                                                                                           tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACt                                                 >  1:1090700/1‑132 (MQ=255)                                                                                           tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTgcg                                          >  1:753500/1‑139 (MQ=255)                                                                                             cgcCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCg                                                                                    <  2:878038/95‑1 (MQ=255)                                                                                             cgcCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCg                                                                                    >  1:878038/1‑95 (MQ=255)                                                                                                     tGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGcc                                <  1:821341/139‑1 (MQ=255)                                                                                                     tGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGcc                                >  2:679288/1‑139 (MQ=255)                                                                                                      gCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCg                               <  2:753500/139‑1 (MQ=255)                                                                                                               cgTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGa                                                                <  2:809134/97‑1 (MQ=255)                                                                                                               cgTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGa                                                                >  1:809134/1‑97 (MQ=255)                                                                                                               cgTATCGCGGTCGTAA‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAg                      >  1:888362/1‑139 (MQ=255)                                                                                                                             aa‑GGGCTTGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCg        <  2:598980/139‑1 (MQ=255)                                                                                                                                    ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCaaa                                                                      >  2:651710/1‑71 (MQ=255)                                                                                                                                    ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCaaa                                                                      <  1:651710/71‑1 (MQ=255)                                                                                                                                    ttGAGTCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTc  >  1:657048/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                      TTGTTCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAA‑GGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTC  >  NC_000913/4515149‑4515417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑