Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,817,796	(T)6→5	coding (705/1353 nt)	chbF ←	phospho‑chitobiase, general 6‑phospho‑beta‑glucosidase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,817,791	0	T	.	100.0%	330.3 / NA	78	coding (710/1353 nt)	chbF	phospho‑chitobiase, general 6‑phospho‑beta‑glucosidase activity
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (38/40);  total (38/40)

TGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAA  >  NC_000913/1817658‑1817921                                                                                                                                      |                                                                                                                                   tGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTTAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATAttttta                                                                                                                               >  2:177800/1‑138 (MQ=255) tGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATAttttta                                                                                                                               >  2:503946/1‑138 (MQ=255) tGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATAttttta                                                                                                                               >  1:1276056/1‑138 (MQ=255)     ccgccTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACaga                                                                                                                          >  1:1375720/1‑139 (MQ=255)       gccTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAgg                                                                                                                        <  1:491227/139‑1 (MQ=255)        ccTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGa                                                                                                                       <  2:1276056/139‑1 (MQ=255)          tttGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACg                                                                                                                     >  1:1049829/1‑139 (MQ=255)             gtagtaTTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGAACGCtt                                                                                                                  >  2:146728/1‑139 (MQ=255)                    tCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTg                                                                                                           >  2:1212168/1‑139 (MQ=255)                           tttCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATAttt                                                                                                                                  <  1:327526/110‑1 (MQ=255)                           tttCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATAttt                                                                                                                                  >  2:327526/1‑110 (MQ=255)                                      aGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATc                                                                                         >  1:1326027/1‑139 (MQ=255)                                      aGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATc                                                                                         >  2:692999/1‑139 (MQ=255)                                      aGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACCAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCt                                                                                                       >  1:1400501/1‑125 (MQ=255)                                      aGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACCAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCt                                                                                                       <  2:1400501/125‑1 (MQ=255)                                         aTCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAg                                                                                      <  2:1326027/139‑1 (MQ=255)                                                  tGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGc                                                                             >  1:1473163/1‑139 (MQ=255)                                                    cTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCaa                                                                           >  1:461844/1‑139 (MQ=255)                                                          gTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGcgcg                                                                     <  1:1212168/139‑1 (MQ=255)                                                             aTACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACt                                                                  <  1:741440/139‑1 (MQ=255)                                                              tACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACtt                                                                 >  1:1237909/1‑139 (MQ=255)                                                                  gcagATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCa                                                             <  2:1375720/139‑1 (MQ=255)                                                                   cagATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAg                                                                                      <  1:180394/113‑1 (MQ=255)                                                                   cagATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAg                                                                                      >  2:180394/1‑113 (MQ=255)                                                                    agATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCAtt                                                           >  2:1430142/1‑139 (MQ=255)                                                                     gATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACagag                                                                                                                         >  2:1451367/1‑76 (MQ=255)                                                                     gATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACagag                                                                                                                         <  1:1451367/76‑1 (MQ=255)                                                                     gATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCAttt                                                          >  2:47763/1‑139 (MQ=255)                                                                      aTAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAg                                                                                      <  2:1185916/110‑1 (MQ=255)                                                                      aTAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAg                                                                                      >  1:1185916/1‑110 (MQ=255)                                                                       tAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAgg                                                                                                                        <  1:411259/75‑1 (MQ=255)                                                                       tAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAgg                                                                                                                        >  2:411259/1‑75 (MQ=255)                                                                        aaGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGa                                                                                                     >  2:876462/1‑93 (MQ=255)                                                                        aaGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGa                                                                                                     <  1:876462/93‑1 (MQ=255)                                                                        aaGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCca                                                                                                <  2:1149693/98‑1 (MQ=255)                                                                        aaGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCca                                                                                                >  1:1149693/1‑98 (MQ=255)                                                                        aaGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGc                                                               >  2:575072/1‑131 (MQ=255)                                                                        aaGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGc                                                               <  1:575072/131‑1 (MQ=255)                                                                            aCATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCTTCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGca                                                   <  2:895847/139‑1 (MQ=255)                                                                            aCATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGca                                                   <  1:692999/139‑1 (MQ=255)                                                                            aCATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGca                                                   <  2:461844/139‑1 (MQ=255)                                                                             cATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCa                                                             <  2:152396/128‑1 (MQ=255)                                                                             cATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCa                                                             <  2:292683/128‑1 (MQ=255)                                                                             cATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCa                                                             >  1:152396/1‑128 (MQ=255)                                                                             cATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCa                                                             >  1:292683/1‑128 (MQ=255)                                                                             cATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGcac                                                  >  2:1441490/1‑139 (MQ=255)                                                                                ggCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCTAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGa                                                                                                     <  1:1108707/85‑1 (MQ=255)                                                                                ggCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGa                                                                                                     >  2:1108707/1‑85 (MQ=255)                                                                                ggCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATc                                               >  2:455613/1‑139 (MQ=255)                                                                                ggCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGACGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAACGCGCGACTTGCCATTTATCAGCACATc                                               >  1:598166/1‑139 (MQ=255)                                                                                 gcagcaGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATcc                                              <  1:1430142/139‑1 (MQ=255)                                                                                     cagATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGccc                                                                                                        >  1:902781/1‑77 (MQ=255)                                                                                     cagATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGccc                                                                                                        <  2:902781/77‑1 (MQ=255)                                                                                     cagATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGca                                                   <  2:1378173/130‑1 (MQ=255)                                                                                     cagATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGca                                                   >  1:1378173/1‑130 (MQ=255)                                                                                     cagATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTaa                                          >  1:473357/1‑139 (MQ=255)                                                                                        aTTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCca                                                                                                <  2:1450842/82‑1 (MQ=255)                                                                                        aTTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCca                                                                                                >  1:1450842/1‑82 (MQ=255)                                                                                        aTTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGa                                       >  1:108023/1‑139 (MQ=255)                                                                                         tttaacgaacgAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGaa                                      <  1:177800/136‑1 (MQ=255)                                                                                         ttCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGaa                                      <  2:1237909/139‑1 (MQ=255)                                                                                         ttCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGaa                                      <  2:690825/139‑1 (MQ=255)                                                                                         ttCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGaa                                      <  2:598166/139‑1 (MQ=255)                                                                                         ttCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGaa                                      <  2:1050455/139‑1 (MQ=255)                                                                                                  cgaaTTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCca                                                                                                >  1:1477626/1‑72 (MQ=255)                                                                                                  cgaaTTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCca                                                                                                <  2:1477626/72‑1 (MQ=255)                                                                                                    aaTTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATACGGt                           <  2:1049829/139‑1 (MQ=255)                                                                                                      ttAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTg                         <  1:470844/139‑1 (MQ=255)                                                                                                              tCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGaa                 <  1:455613/139‑1 (MQ=255)                                                                                                               cACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGa                                                                                                     <  1:681517/54‑1 (MQ=255)                                                                                                               cACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGa                                                                                                     >  2:681517/1‑54 (MQ=255)                                                                                                               cACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCt                                             >  2:683384/1‑110 (MQ=255)                                                                                                               cACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCt                                             <  1:683384/110‑1 (MQ=255)                                                                                                               cACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGaa                                      >  2:1123371/1‑117 (MQ=255)                                                                                                               cACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGaa                                      <  1:1123371/117‑1 (MQ=255)                                                                                                                aCTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACa               <  1:1337016/139‑1 (MQ=255)                                                                                                                aCTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACa               <  1:503946/139‑1 (MQ=255)                                                                                                                          aGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGca                                                   <  2:457992/93‑1 (MQ=255)                                                                                                                          aGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGca                                                   >  1:457992/1‑93 (MQ=255)                                                                                                                          aGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGc                                                    >  2:615413/1‑92 (MQ=255)                                                                                                                          aGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGc                                                    <  1:615413/92‑1 (MQ=255)                                                                                                                            aTCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCgatagata   >  2:865013/1‑139 (MQ=255)                                                                                                                            aTCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGA‑GCCGAACAAATCGATAGATaa  >  1:1209922/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                   TGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAA  >  NC_000913/1817658‑1817921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑