| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,012,553 | Δ1 bp | intergenic (+160/+61) | yqeA → / ← yqeB | putative amino acid kinase/XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,012,552 | 0 | G | . | 98.3% | 225.8 / ‑2.4 | 58 | intergenic (+159/+62) | yqeA/yqeB | putative amino acid kinase/XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (30/27); minor base A (1/0); total (31/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
TACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCC > NC_000913/3012414‑3012677 | tACCATCCGGCCAAAGCTTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCGTGATAAGCGTAGCGCAACAg < 2:999635/139‑1 (MQ=255) tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAAtt > 2:965691/1‑139 (MQ=255) tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAAtt > 2:22108/1‑139 (MQ=255) tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAAtt > 2:1078324/1‑139 (MQ=255) ggCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTgg < 1:63738/139‑1 (MQ=255) ccAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCg > 1:924153/1‑139 (MQ=255) cgcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCg > 2:418920/1‑139 (MQ=255) gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg < 2:924153/139‑1 (MQ=255) gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg < 1:522222/139‑1 (MQ=255) gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg < 1:22108/139‑1 (MQ=255) gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAACTTGGCGTTTGTCATCAGTCTCAGGCCgg < 2:910161/139‑1 (MQ=255) gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATACAATAGAGTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg < 1:425701/139‑1 (MQ=255) gttgATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgggtggg < 1:418920/139‑1 (MQ=255) gcggcgCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTACGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTAGTCATCAGTCTCAGGCCGGGTTGCTATTACCATCCgg < 1:984285/139‑1 (MQ=37) gcgcGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCa > 2:1435344/1‑139 (MQ=255) aGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGc < 1:215853/110‑1 (MQ=255) aGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGc > 2:215853/1‑110 (MQ=255) tCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg > 1:171583/1‑100 (MQ=255) tCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg < 2:171583/100‑1 (MQ=255) cTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTcc > 2:1017567/1‑139 (MQ=255) cATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTgg < 1:192841/66‑1 (MQ=255) cATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTgg > 2:192841/1‑66 (MQ=255) cATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCActtc > 2:1206020/1‑139 (MQ=255) aTCGCAAATTCAATAGATTGCAATGCACTTGTAGCCCTGATCAGCGTAGCGCA‑CTAGCAATTTGACGTTTGTCACCAGTCCCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCActtct > 1:317345/1‑139 (MQ=255) tCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGcc < 2:30869/86‑1 (MQ=255) tCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGcc > 1:30869/1‑86 (MQ=255) cGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGc > 1:615302/1‑121 (MQ=255) cGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGc < 2:615302/121‑1 (MQ=255) cGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCttt > 2:1258404/1‑139 (MQ=255) aTAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTc < 1:965691/139‑1 (MQ=255) gATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACg < 1:1435344/139‑1 (MQ=255) tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGtcat < 1:697630/57‑1 (MQ=255) tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGtcat > 2:697630/1‑57 (MQ=255) tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTcc < 1:113347/112‑1 (MQ=255) tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTcc > 2:113347/1‑112 (MQ=255) tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGccc > 2:423791/1‑139 (MQ=255) tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGccc > 1:90480/1‑139 (MQ=255) aaTGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTc < 1:692791/130‑1 (MQ=255) aaTGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTc > 2:692791/1‑130 (MQ=255) tGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATg > 2:776950/1‑139 (MQ=255) cACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAgg < 2:1340918/60‑1 (MQ=255) cACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAgg > 1:1340918/1‑60 (MQ=255) aCTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCttt > 2:251979/1‑124 (MQ=255) aCTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCttt < 1:251979/124‑1 (MQ=255) aCTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCAt < 1:1078324/139‑1 (MQ=255) tGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCaa < 1:423791/139‑1 (MQ=255) ggCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCgg < 2:279330/74‑1 (MQ=255) ggCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCgg > 1:279330/1‑74 (MQ=255) ggCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAAcatc > 1:710081/1‑139 (MQ=255) ggCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTATCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAAcatc > 1:891096/1‑139 (MQ=255) gATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGcc < 1:1048303/86‑1 (MQ=255) gATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGcc > 2:1048303/1‑86 (MQ=255) gATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTaa > 2:992390/1‑139 (MQ=255) aGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGcc < 1:1206020/139‑1 (MQ=255) cGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg > 1:1244393/1‑45 (MQ=21) cGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg < 2:1244393/45‑1 (MQ=18) gcgcATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg > 2:1044676/1‑41 (MQ=21) gcgcATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg < 1:1044676/41‑1 (MQ=21) cgcATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg > 2:951853/1‑40 (MQ=21) | TACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCC > NC_000913/3012414‑3012677 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |