| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,511,746 | (A)8→7 | coding (599/906 nt) | yhfZ ← | putative DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,511,746 | 0 | A | . | 81.8% | 157.4 / 50.2 | 66 | coding (599/906 nt) | yhfZ | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (6/6); new base . (24/30); total (30/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01 | |||||||||||
CCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAA‑AA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGCGCGAGGCATAA > NC_000913/3511618‑3511882 | agcatggtcagttcgttttccgccaccacgttccagatcaccgcatcgacatcgcctttaacaatgcgttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATg > 1:742813‑M1/122‑139 (MQ=255) ttcgttttccgccaccacgttccagatcaccgcatcgacatcgcctttaacaatgcgttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATc < 2:1254949‑M1/29‑1 (MQ=255) ttcgttttccgccaccacgttccagatcaccgcatcgacatcgcctttaacaatgcgttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATc < 1:996653‑M1/29‑1 (MQ=255) tttccgccaccacgttccagatcaccgcatcgacatcgcctttaacaatgcgttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATccgccg > 2:223502‑M1/106‑139 (MQ=255) tccgccaccacgttccagatcaccgcatcgacatcgcctttaacaatgcgttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGaa > 2:93786‑M1/104‑139 (MQ=255) atcaccgcatcgacatcgcctttaacaatgcgttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg > 2:996653‑M1/86‑139 (MQ=255) ccgcatcgacatcgcctttaacaatgcgttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCt < 1:763063‑M1/58‑1 (MQ=255) cgcatcgacatcgcctttaacaatgcgttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑A‑ACCGGTCATGATTTTTTGATCCCCCGAACGGTTATCCAcccccaccccctt < 2:761968‑M1/59‑7 (MQ=255) cgcatcgacatcgcctttaacaatgcgttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTAt < 1:1228023‑M1/43‑1 (MQ=255) cgcatcgacatcgcctttaacaatgcgttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTAt > 2:1228023‑M1/81‑123 (MQ=255) cgcatcgacatcgcctttaacaatgcgttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt < 2:119338‑M1/59‑1 (MQ=255) cgcatcgacatcgcctttaacaatgcgttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt < 2:157946‑M1/59‑1 (MQ=255) cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAA‑AA‑ACATCGGTCATGATTTTCTGATCCGCCGAACgg > 2:468477/1‑118 (MQ=255) cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAA‑AA‑ACATCGGTCATGATTTTCTGATCCGCCGAACgg < 1:468477/118‑1 (MQ=255) cctttaacaatgcgttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGAc < 2:243554‑M1/72‑1 (MQ=255) tccTTTAAGAAAGGCTTCTAAAGTCTCGCGATAATATAGATCGACGCGTTCCACATCCCTCCCGCCAAAAA‑AACACATCCGTCATGATTTTCTTATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa < 1:971759/138‑1 (MQ=255) tgcgttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGt < 2:696425‑M1/14‑1 (MQ=255) tgcgttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGt > 1:696425‑M1/58‑71 (MQ=255) tgcgttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc < 2:710566‑M1/82‑1 (MQ=255) tgcgttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc < 1:375069‑M1/82‑1 (MQ=255) gcgttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACg < 2:876208‑M1/83‑1 (MQ=255) gttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc > 1:415909‑M1/55‑139 (MQ=255) ttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATc < 1:296487‑M1/44‑1 (MQ=255) ttgtaaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATc > 2:296487‑M1/54‑97 (MQ=255) taaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAAt < 2:169318‑M1/89‑1 (MQ=255) aaactctcgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATc > 2:375069‑M1/50‑139 (MQ=255) ctctcgtgataagagagatctactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAAc > 1:581122‑M1/47‑139 (MQ=255) cgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGCCTCGCCTTTACGGCAAATCAACTGGt < 2:1408308‑M1/97‑1 (MQ=255) cgtgataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGAGCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGt < 1:129425‑M1/97‑1 (MQ=255) ataagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc > 2:129425‑M1/39‑139 (MQ=255) aagagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc > 1:169318‑M1/37‑139 (MQ=255) tAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAA‑AA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc < 1:1164658/139‑1 (MQ=255) agagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt > 2:3310‑M1/36‑108 (MQ=255) agagagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt < 1:3310‑M1/73‑1 (MQ=255) agagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc < 1:81678‑M1/101‑1 (MQ=255) agagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc > 2:81678‑M1/34‑134 (MQ=255) agagatcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCaa < 2:786886‑M1/106‑1 (MQ=255) atcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc < 2:592415‑M1/101‑1 (MQ=255) atcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc > 1:592415‑M1/30‑130 (MQ=255) atcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa < 2:757096‑M1/110‑1 (MQ=255) atcgactcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa < 1:1093479‑M1/110‑1 (MQ=255) tcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGtgtg < 2:1426347‑M1/116‑1 (MQ=255) tcgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGtgtg > 1:1120666‑M1/24‑139 (MQ=255) cgttccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGc > 1:1254949‑M1/23‑139 (MQ=255) cTCGTTCCACATCACTACCGCCAAAAATAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGgtgt > 2:738695/1‑139 (MQ=255) tCGTTCCACATCACTACCGCCAAAAATAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGtgtg < 1:738695/139‑1 (MQ=255) tccacatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGc < 1:733835‑M1/120‑1 (MQ=255) acatcactaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGcccc > 2:763063‑M1/17‑139 (MQ=255) actaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGcc < 1:460939‑M1/121‑1 (MQ=255) actaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGcc > 2:460939‑M1/12‑132 (MQ=255) ctgccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTcc < 1:1047162‑M1/129‑1 (MQ=255) ctaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa < 1:132783‑M1/71‑1 (MQ=255) ctaccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa > 2:132783‑M1/11‑81 (MQ=255) taccgccaaAAAAA‑AA‑ACATCGGTCATGATTTTCTGATccgccg > 1:124748‑M1/10‑44 (MQ=255) taccgccaaAAAAA‑AA‑ACATCGGTCATGATTTTCTGATccgccg < 2:124748‑M1/35‑1 (MQ=255) taccgccaaAAAAA‑AA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc > 2:1112728‑M1/10‑111 (MQ=255) taccgccaaAAAAA‑AA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc < 1:1112728‑M1/102‑1 (MQ=255) taccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACgg < 1:894194‑M1/39‑1 (MQ=255) taccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACgg > 2:894194‑M1/10‑48 (MQ=255) taccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg > 1:386838‑M1/10‑63 (MQ=255) taccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg < 2:386838‑M1/54‑1 (MQ=255) taccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa > 2:1100343‑M1/10‑80 (MQ=255) taccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa < 1:1100343‑M1/71‑1 (MQ=255) ccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACgg < 1:731785‑M1/39‑1 (MQ=255) ccgccaaAAAAA‑AA‑‑CATCGGTCATGATTTTCTGATCCGCCGAACgg > 2:731785‑M1/8‑46 (MQ=255) gccaAAAAA‑AA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAgcgc < 2:309067‑M1/135‑1 (MQ=255) ccaAAAAA‑AA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGcgcg > 2:1060765‑M1/4‑139 (MQ=255) AA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGCGCGAGGCATaa > 1:757096‑M1/1‑139 (MQ=255) | CCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAA‑AA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGCGCGAGGCATAA > NC_000913/3511618‑3511882 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |